Primer Designing

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Primer Designing

  1. 1. Primer Design
  2. 2. 1. Gene Runner 2. Oligo 6 4. Manual 3. Primer 3(on line)
  3. 3. Gene Runner 1.Sequence Insertion
  4. 10. Oligo 6
  5. 17. Manual 1. Reference sequence & other sequences (NCBI) 2. Alignment (Mega 4) 3. Sequence selection (In selected gene) 4. Blast (NCBI) 5. TM ( oligo analyzer , oligo6, gene runner) 6. Secondary structures (loop, hairpin,…) 7. False priming (oligo 6)
  6. 18. HCV Genome
  7. 19. Alignment
  8. 21. Current oligo length Position number
  9. 22. Non specific band
  10. 27. - Length <ul><li>Miss match </li></ul><ul><li>*5-7nt from 3’ end </li></ul>

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