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Nephlometry &  turbidimetry
Nephlometry &  turbidimetry
Nephlometry &  turbidimetry
Nephlometry &  turbidimetry
Nephlometry &  turbidimetry
Nephlometry &  turbidimetry
Nephlometry &  turbidimetry
Nephlometry &  turbidimetry
Nephlometry &  turbidimetry
Nephlometry &  turbidimetry
Nephlometry &  turbidimetry
Nephlometry &  turbidimetry
Nephlometry &  turbidimetry
Nephlometry &  turbidimetry
Nephlometry &  turbidimetry
Nephlometry &  turbidimetry
Nephlometry &  turbidimetry
Nephlometry &  turbidimetry
Nephlometry &  turbidimetry
Nephlometry &  turbidimetry
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Nephlometry & turbidimetry

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Nephlometry. & turbidimetry - PPT

Nephlometry. & turbidimetry - PPT

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  • 1.
    • TURBIDIMETRY AND NEPHELOMETRY
  • 2. TURBIDIMETRY AND NEPHELOMETRY
    • Presented By -
    • Mr. Shaise Jacob
    • Faculty
    • Nirmala College of Pharmacy
    • Muvattupuzha, Kerala
    • India
    • Email – jacobshaise@gmail.com
  • 3.
    • » Analysis of Suspensions
    • NEPHELOMETRY – Measurement of scattered light
    • ( opalescence)
    • TURBIDIMETRY - Measurement of transmitted
    • light ( turbidity )
    • » absorption, refraction & reflection -> transmitted
    • Tyndall Effect ..
    • Scattering of light-
  • 4.  
  • 5.  
  • 6.  
  • 7.
    • Turbidimetry
    • Colorimetry
    • Nephelometry
    • Fluorimetry
  • 8. NEPHELOMETRY
    • ↓ concentration, uniform scattering, intensity of scatted light proportional to conc. measured at 90 0
    • TURBIDIMETRY
    • ↑ concentration, scattering not uniform, intensity of transmitted light measured at 180 0
    • Factors affecting measurements
    • Concentration, Particle geometry, WL of incident light, RI difference
  • 9. Nephelometer
  • 10. Science of Colorimeter
  • 11.
    • Tungsten lamp
    • Filters- Turbidimeter (blue filter or 530 nm)
    • Nephelometer (visible filter)
    • Sample cells
    • Detectors (photometric)
    • SOP
    • Nephelometer: Flourimeter -> Nephelometer by using a visible filter as secondary filter
  • 12. TURBIDIMETER
  • 13.
    • Difference:
    • Scattering of radiation
    • Difference:
    • Absorption of radiation
    • TURBIDIMETER
    • Similarity:
    • transmitted light measured
    • measured at 180 0
    • COLORIMETER
    • Similarity:
    • transmitted light measured
    • measured at 180 0
  • 14. Difference: » emitted radiation measured » emitted radiation – longer WL than incident light
    • FLOURIMETRY
    • Similarity:
    • Emergent radiation measured at 90 0
    Difference: » Scattered radiation measured » emitted radiation – same WL as that of incident light
    • NEPHELOMETRY
    • Similarity:
    • Emergent radiation measured at 90 0
  • 15. APPLICATIONS
    • Analysis of water
    • clarity, conc. of ions
    • Determination of CO 2
    • Determination of inorganic substances
    • Sulphate – barium chloride
    • Ammonia – Nesslers reagent
    • Phosphorus – Strychine molybedate
    • Biochemical Analysis
    • 5. Quantitative Analysis – (ppm level)
  • 16.
    • 6. Miscellaneous
    • Water treatment plants, sewage work, refineries, paper industry
    • 7. Atmospheric pollution
    • smokes & fogs
    • 8. Determination of mole. Wt of high polymers
    • 9. Phase titration
    • NEPHLOTURBIDIMETER
    • Two detectors
  • 17.  
  • 18.  
  • 19.  
  • 20.  

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