Lec 2 plant cell structures
Upcoming SlideShare
Loading in...5
×
 

Like this? Share it with your network

Share

Lec 2 plant cell structures

on

  • 554 views

Bio 103: Fundamentals in Plant Biology.

Bio 103: Fundamentals in Plant Biology.

Statistics

Views

Total Views
554
Views on SlideShare
554
Embed Views
0

Actions

Likes
0
Downloads
11
Comments
0

0 Embeds 0

No embeds

Accessibility

Categories

Upload Details

Uploaded via as Adobe PDF

Usage Rights

© All Rights Reserved

Report content

Flagged as inappropriate Flag as inappropriate
Flag as inappropriate

Select your reason for flagging this presentation as inappropriate.

Cancel
  • Full Name Full Name Comment goes here.
    Are you sure you want to
    Your message goes here
    Processing…
Post Comment
Edit your comment

Lec 2 plant cell structures Presentation Transcript

  • 1. Plant Cell Structures
  • 2. Cell Structure In 1655, the English scientist Robert Hooke coined the term “cellulae” for the small box-like structures he saw while examining a thin slice of cork under a microscope.
  • 3. TWO TYPES OF CELLS Prokaryotes •single compartment and surrounded by the plasma membrane •lacks a defined nucleus •simple internal organization •ex. bacteria, blue-green algae (cyanobacteria) Eukaryotes • •contain a defined membrane-bound nucleus • extensive internal membranes that enclose compartments main parts: plasma membrane, cytoplasm and nucleus • ex. all members of the plant and animal kingdom, fungi (molds* and yeasts**), protozoan*
  • 4. Prokaryotes Eukaryotes Cell membrane Ribosomes Cell wall Nucleus Endoplasmic reticulum Golgi apparatus Lysosomes Vacuoles Mitochondria Cytoskeleton Animal Cells Plant Cells Lysosomes Cell membrane Ribosomes Nucleus Endoplasmic reticulum Golgi apparatus Vacuoles Mitochondria Cytoskeleton Cell Wall Chloroplasts
  • 5. The Structure of Eukaryotic Plant Cell • A living plant cell is composed of three main parts namely: cell wall, protoplast and inclusions
  • 6. 1. The Cell Wall • The outermost part of the plant cell • Non-living structure with main chemical component cellullose (a polysaccharide) • Other substances which may form part of the cell wall are lignin, suberin and cutin • Between neighboring cell walls is a cementing intercellular layer composed of pectin substance • The nonliving layer is often referred to as the middle lamella
  • 7. PROTOPLASM a mass of proteins, lipids, nucleic acids, and water within a cell; except for the wall, everything in the cell is protoplasm • Portions of the protoplast is organized into protoplasmic bodies with specific functions and are generally referred to as ORGANELLES • The protoplast is composed of two main regions, namely: an outer region called as the CYTOPLASM and an inner region as the NUCLEUS
  • 8. • Is bounded by a protoplasmic membrane called the plasma membrane, plasmalemma or cell membrane • The plasma membrane is a selectively permeable protoplasmic membrane which regulates the entry and exit of materials in a cell
  • 9. Other main cytoplasmic structures of plant cells: 1. Mitochondria 2. Ribosomes 3. Endoplasmic Reticulum (ER) 4. Golgi bodies or dictyosomes 5. Lysosomes 6. Plastids 7. Microtubules
  • 10. Mitochondria • Mitochondria are found in PLANT and animal cells. • Sites of cellular respiration, ATP synthesis • Bound by a double membrane surrounding fluid-filled matrix. • The inner membranes of mitochondria are cristae • The matrix contains enzymes that break down carbohydrates and the cristae house protein complexes that produce ATP THE POWER HOUSE OF THE CELL ATP
  • 11. Ribosomes • Granular structures visible under electron microscope • Protein synthesis • Ribosomes are composed of a large subunit and a small subunit. • Ribosomes can be found alone in the cytoplasm, in groups called polyribosomes, or attached to the endoplasmic reticulum. • Ribosomes are RNA-protein complexes composed of two subunits that join and attach to messenger RNA. – site of protein synthesis – assembled in nucleoli
  • 12. Endomembrane System • Compartmentalizes cell, channeling passage of molecules through cell’s interior. – Endoplasmic reticulum • Rough ER - studded with ribosomes • Smooth ER - few ribosomes
  • 13. Rough ER • Rough ER is especially abundant in cells that secrete proteins. – As a polypeptide is synthesized on a ribosome attached to rough ER, it is threaded into the cisternal space through a pore formed by a protein complex in the ER membrane. – As it enters the cisternal space, the new protein folds into its native conformation. – Most secretory polypeptides are glycoproteins, proteins to which a carbohydrate is attached. – Secretory proteins are packaged in transport vesicles that carry them to their next stage.
  • 14. Smooth ER • The smooth ER is rich in enzymes and plays a role in a variety of metabolic processes.
  • 15. The Golgi bodies/ dictyosomes • The Golgi body is the shipping and receiving center for cell products. – Many transport vesicles from the ER travel to the Golgi apparatus for modification of their contents. – The Golgi is a center of manufacturing, warehousing, sorting, and shipping. – The Golgi apparatus consists of flattened membranous sacs— cisternae—looking like a stack of pita bread. – The Golgi sorts and packages materials into transport vesicles.
  • 16. Functions Of The Golgi Bodies TEM of Golgi apparatus cis face (“receiving” side of Golgi apparatus) Vesicles move from ER to GolgiVesicles also transport certain proteins back to ER Vesicles coalesce to form new cis Golgi cisternae Cisternal maturation: Golgi cisternae move in a cis- to-trans direction Vesicles form and leave Golgi, carrying specific proteins to other locations or to the plasma mem- brane for secretion Vesicles transport specific proteins backward to newer Golgi cisternae Cisternae trans face (“shipping” side of Golgi apparatus) 0.1 0 µm1 6 5 2 3 4 Golgi apparatus
  • 17. Lysosomes and Peroxisomes • Lysosomes – vesicle containing hydrolytic or digestive enzymes that break down food/foreign particles • Peroxisomes - contain enzymes that catalyze the removal of electrons and associated hydrogen atoms
  • 18. Lysosome • Rounded or iregularly- shaped organelles bounded by a single membrane • Said to play important role in the destruction of worn-out or defective parts of the cell • Probably belong to a group of structures like microbodies (a) Phagocytosis: lysosome digesting food 1 µm Lysosome contains active hydrolytic enzymes Food vacuole fuses with lysosome Hydrolytic enzymes digest food particles Digestion Food vacuole Plasma membrane Lysosome Digestive enzymes Lysosome Nucleus
  • 19. Plastids • Rounded, oval or irregularly-shaped protoplasmic bodies of three main parts: • Leucoplast • Chroloplast • Chromoplast
  • 20. Leucoplast • Colorless plastids • Some involved in food storage • If associated with the starch storage, they are referred to as amyloplasts • if associated with oil storage= elaioplasts • With protein storage = aleurone-plasts
  • 21. Potato (Solanum tuberosum)starch grains amyloplasts
  • 22. Chloroplasts • A chloroplast is bounded by two membranes enclosing a fluid-filled stroma that contains enzymes. • Membranes inside the stroma are organized into thylakoids that house chlorophyll. • Chlorophyll absorbs solar energy and carbohydrates are made in the stroma.
  • 23. Digman cells (Hydrilla verticillata)
  • 24. Chromoplast • Colored plastids other than green • The main pigments are the carotenoids Bell pepper
  • 25. • the thickest fibers, are hollow rods about 25 microns in diameter. – are constructed of the globular protein, tubulin, and they grow or shrink as more tubulin molecules are added or removed. • They move chromosomes during cell division. Fig. 7.21b Microtubules
  • 26. • long, hollow cylinders and made-up of protein tubulin • outer diameter of 25nm • much more rigid • long and straight and typically have one end attached to a single microtubule-organizing center (MTOC) called a centrosome
  • 27. The Nucleus • Repository for genetic material • Chromatin: DNA and proteins • Nucleolus: Chromatin and ribosomal subunits - region of intensive ribosomal RNA synthesis. darkly staining rounded bodies rich in ribosomal RNA, the type of RNA used in the formation of ribosomes • Nuclear envelope: Surface of nucleus bound by two phospholipid bilayer membranes - Double membrane with pores • Nucleoplasm: semifluid medium inside the nucleus
  • 28. Nucleus
  • 29. Nucleus – Nickname: “The Control Center” – Directs cell activities • Separated from cytoplasm by nuclear membrane • Contains genetic material - DNA
  • 30. The Nucleus And The Nuclear Envelope
  • 31. Chromosomes • DNA of eukaryotes is divided into linear chromosomes. – Exist as strands of chromatin, except during cell division – Histones associated packaging proteins
  • 32. 3. The Inclusions • Refer to the nonprotoplasmic structures found within the protoplast • Among these are the VACUOLES which are fluid-filled structures • The fluid vacuole commonly referred to as the cell sap • Contains various dissolved substances such as anthocyanins (water-soluble pigments) and various metabolites (e.g. Sugars, inorganic salts, organic acids, alkaloids)
  • 33. Cont... • Other inclusions are waste products which may be in a form of crystals • The crystals may be contained in vacoules and in the cytoplasm • Plant cell may start with many but small vacuoles • Upon maturity, the small vacuoles may coalesce to form bigger but fewer vacuoles • Eventually, a plant cell may have but a SINGLE LARGE CENTRAL VACUOLE • Some biologist would consider vacuole as an oganelle. As such the membrane-bounded structure containing the cell sap.
  • 34. Crystals in Plant Cells • Many plant cells contain crystals which are a product of metabolism • There are many forms • Most common are composed of calcium oxide and calcium carbonate
  • 35. Calcium oxalate crystals • Generally found in the vacuoles and are as follows: 1. Raphides – needle like crystals which occur singly or in groups or bundles as in gabi and other succulent plants 2. Prismatic – prism-like or pyramid-like crystals found in leaves of begonia and bangka-bangkaan (Rhoeo discolor) 3. Rosette – aggregate of crystals which has flower-like apperance in santan (Ixora sp.) and stem of Kutsarita plant
  • 36. Crystalline bodies in plant cells Hypoestes phyllostachya, the polka dot plant
  • 37. Crystalline bodies in plant cells onion scales
  • 38. Crystalline bodies in plant cells
  • 39. Calcium carbonate crystals • Cystotith- grape-like as seen in a hypodermal cell of the leaf of Indian rubber tree or ampalaya-like plant.
  • 40. BASIC TYPES OF CELLS • Despite the diversity of types of stems that have originated by natural selection, all share a basic, rather simple organization. • The same is true for leaves and roots. • Although we might suspect that numerous types of cells are present within a plant, actually the various kinds of plant cells are customarily grouped into three classes based on the nature of their walls: Parenchyma, Collenchyma, and Sclerenchyma.
  • 41. 1. PARENCHYMA • Parenchyma cells have only primary walls that remain thin. • Parenchyma tissue is a mass of parenchyma cells. This is the most common type of cell and tissue, constituting all soft parts of a plant. • Parenchyma cells are active metabolically and usually remain alive once they mature. • Numerous subtypes are specialized for particular tasks:
  • 42. Parenchyma cells of Geranium spp.; their walls (green) are thin, and their vacuoles are large and full of watery contents that did not stain. Nuclei were present in all cells,
  • 43. Subtypes of Parenchyma Cells A. Chlorenchyma cells B. Glandular cells C.Transfer cells D.(Aerenchyma cells)
  • 44. A. Chlorenchyma cells • Chlorenchyma cells are parenchyma cells involved in photosynthesis • they have an abundance of chloroplasts, and the thinness of the wall is advantageous for allowing light and carbon dioxide to pass through to the chloroplasts. • Other types of pigmented cells, as in flower petals and fruits, also must be parenchyma cells with thin walls that permit the pigments in the protoplasm to be seen.
  • 45. Chlorenchyma cells from a leaf of privet- Ligustrum vulgare L. -Intercellular spaces, permit CO2 rapid diffusion in the leaf
  • 46. http://academic.kellogg.edu/herbrandsonc/bio111/tissue.htm
  • 47. B. Glandular cells • Glandular cells that secrete nectar, fragrances, mucilage, resins, and oils are also parenchyma cells; they typically contain few chloroplasts but have elevated amounts of dictyosomes and endoplasmic reticulum. • They must transport large quantities of sugar and minerals into themselves, transform them metabolically, then transport the product out.
  • 48. Mauseth, J.
  • 49. C. Transfer cells • Transfer cells are parenchyma cells that mediate the short- distance transport of material by means of a large, extensive plasma membrane capable of holding numerous molecular pumps. • Unlike animal cells, plant cells cannot form folds or projections of their plasma membranes; instead, transfer cells increase their surface area by having extensive knobs, ridges, and other ingrowths on the inner surface of their walls • Because the plasma membrane follows the contour of all these, it is extensive and capable of large-scale molecular pumping.
  • 50. Transfer cells in the salt gland of Frankenia grandifolia. The wall ingrowths increase the surface area of the cell membrane, providing more room for salt-pumping proteins in the membrane. W. W. Thomson and R. Balsamo, University of California, Riverside
  • 51. D. (Aerenchyma cells) • Parenchyma tissue with extensive connected air spaces. • refers to spaces or air channels in the leaves, stems and roots of some plants, which allows exchange of gases between the shoot and the root. • The channels of air-filled cavities provide a low- resistance internal pathway for the exchange of gases such as oxygen and ethylene between the plant above the water and the submerged tissues. • Aerenchyma is widespread in aquatic and wetland plants which must grow in hypoxic soils
  • 52. Stem cross-section of Peperomia. The inner part of the stem is mostly parenchyma cells.
  • 53. • Some parenchyma cells function by dying at maturity. • Structures such as stamens and some fruits must open and release pollen or seeds; the opening may be formed by parenchyma cells that die and break down or are torn apart. • Large spaces may be necessary inside the plant body; some of these are formed when the middle lamella decomposes and cells are released from their neighbors. • In other cases, the space is formed by the degeneration of parenchyma cells. • In a few species, such as milkweeds, as parenchyma cells die, their protoplasm is converted metabolically into mucilage or a milky latex.
  • 54. • Parenchyma tissue that conducts nutrients over long distances is phloem • Parenchyma cells are relatively inexpensive to build because little glucose is expended in constructing the wall's cellulose and hemicellulose. • Each molecule incorporated into a wall polymer cannot be used for other purposes such as the generation of ATP or the synthesis of proteins. • Consequently, it is disadvantageous to use a cell with thick walls any time one with thin walls would be just as functional.
  • 55. 2. COLLENCHYMA CELLS • Collenchyma cells have a primary wall that remains thin in some areas but becomes thickened in other areas, most often in the corners. • The nature of this wall is important in understanding why it exists and how it functions in the plant. • Like clay, the wall of collenchyma exhibits plasticity, the ability to be deformed by pressure or tension and to retain the new shape even if the pressure or tension ceases.
  • 56. • Collenchyma is present in elongating shoot tips that must be long and flexible, such as those of vining plants like grapes, as a layer just under the epidermis or as bands located next to vascular bundles, making the tips stronger and more resistant to breaking. • But the tips are still capable of elongating because collenchyma can be stretched. • In species whose shoot tips are composed only of weak parenchyma, the tips are flexible and delicate and often can be damaged by wind; the elongating portion must be very short or it simply buckles under its own weight.
  • 57. Stem cross-section of Peperomia. Masses of collenchyma cells often occur in the outer parts of stems and leaf stalks. The collenchyma forms a band about 8 to 12 cells thick.
  • 58. • It is important to think about the method by which collenchyma provides support. • If a vine or other collenchyma-rich tissue is cut off from its water supply, it wilts and droops; the collenchyma is unable to hold up the stem. • Parenchyma cells are needed in the inner tissues for support. Collenchyma and turgid parenchyma work together like air pressure and a tire: The tire or inner tube is extremely strong but is useless for support without air pressure. • Similarly, air pressure is useless unless it is confined by a container. • In stems, the tendency for parenchyma to expand is counterbalanced by the resistance of the collenchyma, and the stem becomes rigid.
  • 59. The shoot tips of long vines need the plastic support of collenchyma while their stems are elongating
  • 60. • Because the walls of collenchyma cells are thick, they require more glucose for their production. • Collenchyma is usually produced only in shoot tips and young petioles, where the need for extra strength justifies the metabolic cost. • Subterranean shoots and roots do not need collenchyma because soil provides support, but the aerial roots of epiphytes such as orchids and philodendrons have a thick layer of collenchyma
  • 61. 3. SCLERENCHYMA CELLS • The third basic type of cell and tissue, sclerenchyma, has both a primary wall and a thick secondary wall that is almost always lignified. • These walls have the property of elasticity: They can be deformed, but they snap back to their original size and shape when the pressure or tension is released. • Sclerenchyma cells develop mainly in mature organs that have stopped growing and have achieved their proper size and shape.
  • 62. A stem of bamboo was treated with a mixture of nitric acid and chromic acid to
  • 63. These are sclereids; they are more or less cuboidal, definitely not long like fibers. These have remained alive at maturity, and nuclei & cytoplasm are visible in
  • 64. astrosclereids (star-shaped sclereids), shines brightly because its cellulose molecules are packed in a tight, crystalline form, giving the wall extra strength .
  • 65. • Deforming forces such as wind, animals, or snow would probably be detrimental. • If mature organs had collenchyma for support, they would be reshaped constantly by storms or animals, which of course would not be optimal. • For example, while growing and elongating, a young leaf must be supported by collenchyma if it is to continue to grow. • But once it has achieved its mature size and shape, some cells of the leaf can mature into sclerenchyma and provide elastic support that maintains the leaf's shape. • Unlike collenchyma, sclerenchyma supports the plant by its strength alone; if sclerenchyma-rich stems are allowed to wilt, they remain upright and do not droop.
  • 66. • Parenchyma and collenchyma cells can absorb water so powerfully that they swell and stretch the wall, thereby growing; sclerenchyma cell walls are strong enough to prevent the protoplast from expanding. • The rigidity of sclerenchyma makes it unusable for growing • shoot tips because it would prevent further shoot elongation. • Sclerenchyma cells are of two types—conducting sclerenchyma and mechanical sclerenchyma. • The mechanical sclerenchyma is subdivided into long fibers and short sclereids , both of which have thick secondary walls. • Because fibers are long, they are flexible and are most often found in areas where strength and elasticity are important.
  • 67. • The wood of most flowering plants contains abundant fibers, and their strength supports the tree while their elasticity allows the trunk and branches to sway in the wind without breaking (usually) or becoming permanently bent . • The fiber-rich bark is important not in holding up the tree but in resisting insects, fungi, and other pests. • Sclereids are short and more or less isodiametric(cuboidal). • Because sclereids have strong walls oriented in all three dimensions, sclerenchyma tissue composed of sclereids is brittle and inflexible.
  • 68. Mauseth, J. SUMMARY
  • 69. Parenchyma  This tissue is composed of large, thin walled cells having a single large vacuole.  This tissue is found in the soft parts of the plant like the cortex(outer region) and pith(inner region) of the roots and stems.  The cells present in these tissues are living.  These cells store food for the plant, and thus they also provide temporary support for the plants.  When parenchymatous cells are present in leaves, they sometimes contain chlorophyll, and thus are green in colour. This tissue is then referred to as chlorenchyma.  Potatoes are made up of mainly Parenchyma tissue cells.
  • 70. Collenchyma  This tissue is made up of cells from the parenchyma tissue which become elongated and have thick cell walls at the corners. This diagram illustrates the difference between parenchyma and collenchyma cells. The collenchyma cells are elongated and thick at the corners.  This tissue is found mainly in the leaf stalks and below the epidermis of stems.  It gives support for the parts of a plant like leaves, stems, branches.
  • 71. Sclerenchyma  Sclerenchyma is made up of thin, narrow and long cells having very thick cell walls due to deposition of lignin.  These cells are thin because they are all dead cells, having no functions to perform and so no organelles inside the cell require space.  They are thick at the cell walls and thus these cells provide rigid support for the plant as they are hard and supportive. That is why the tissue is named "Sclerenchyma", as scleros means hard.  Sclerenchyma tissue is present in stems of plants and the veins of leaves in plants. It provides strength to plant parts.