Bio-sensors  Presented byVIVEK BHANDARI     LBEF
CONTENTS• Introduction• Principles of Detection• Applications
INTRODUCTION• A biosensor is an analytical device for the  detection of an analyte that combines a  biological component w...
Bio-sensor
How does a Bio-sensor works?
Principles of DetectionPhotometricElectrochemicalIon Channel SwitchOthers
Electrochemical
Electrochemical biosensors• normally based on enzymatic catalysis of a reaction that  produces or consumes electrons (such...
Photometric
Photometric• Many optical biosensors based on the phenomenon of  surface plasmon resonance (SPR) are evanescent wave  tech...
Piezoelectric
Others• Piezoelectric sensors utilise crystals which  undergo an elastic deformation when an  electrical potential is appl...
Bio-electrical analysis
Applications• Glucose monitoring in diabetes patients.• Other medical health related targets.• Environmental applications....
Blood Glucose Testing
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Biosensors

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Biosensors

  1. 1. Bio-sensors Presented byVIVEK BHANDARI LBEF
  2. 2. CONTENTS• Introduction• Principles of Detection• Applications
  3. 3. INTRODUCTION• A biosensor is an analytical device for the detection of an analyte that combines a biological component with a physicochemical detector component.
  4. 4. Bio-sensor
  5. 5. How does a Bio-sensor works?
  6. 6. Principles of DetectionPhotometricElectrochemicalIon Channel SwitchOthers
  7. 7. Electrochemical
  8. 8. Electrochemical biosensors• normally based on enzymatic catalysis of a reaction that produces or consumes electrons (such enzymes are rightly called redox enzymes).• The sensor substrate usually contains three electrodes; a reference electrode, a working electrode and a counter electrode.• The target analyte is involved in the reaction that takes place on the active electrode surface, and the reaction may cause either electron transfer across the double layer (producing a current) or can contribute to the double layer potential (producing a voltage
  9. 9. Photometric
  10. 10. Photometric• Many optical biosensors based on the phenomenon of surface plasmon resonance (SPR) are evanescent wave techniques.• This utilises a property of gold and other materials; specifically that a thin layer of gold on a high refractive index glass surface can absorb laser light, producing electron waves (surface plasmons) on the gold surface.• This occurs only at a specific angle and wavelength of incident light and is highly dependent on the surface of the gold, such that binding of a target analyte to a receptor on the gold surface produces a measurable signal.
  11. 11. Piezoelectric
  12. 12. Others• Piezoelectric sensors utilise crystals which undergo an elastic deformation when an electrical potential is applied to them. An alternating potential (A.C.) produces a standing wave in the crystal at a characteristic frequency.
  13. 13. Bio-electrical analysis
  14. 14. Applications• Glucose monitoring in diabetes patients.• Other medical health related targets.• Environmental applications.• Detection of pathogens .• Determining levels of toxic substances before and after bioremediation.• Detection and determining of organophosphate .• Determination of drug residues in food.• Drug discovery and evaluation of biological activity of new compounds.
  15. 15. Blood Glucose Testing

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