Adjacent nucleotides join/form a bond with the help of DNA polymer ase (“proof-reader”) to form new sugar-phosphate backbones resulting in 2 identical DNA molecules. This is referred to as semi-conservative .
DNA/genome from two different organisms are spliced together to obtain a desired trait/characteristic or perform a specific function; the manipulation of a genome from two or more sources, such as bacteria, plants or animals.
E.g. Bacteria nucleic acid containing a human hormone gene and producing biotechnology products, i.e. insulin, growth hormone.