25/01/2012             Lecture 9 Animal Cell Biotechnology           Scaling up the production process                    ...
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Lecture 91c scaling up

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Industrial Microbiology Dr. Butler 2011

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Lecture 91c scaling up

  1. 1. 25/01/2012 Lecture 9 Animal Cell Biotechnology Scaling up the production process Lecture 9 Animal Cell Biotechnology Scaling up the production process Motor driven magnetic stirrer. Removable motor that fits on top of the drive shaft, removed before washing and autoclaving. Marine impeller Lecture 9 Animal Cell Biotechnology Lecture 9 Animal Cell Biotechnology Scaling up the production process Scaling up the production processTemperature• thermal probe inserted through headplate into culture• maintain temperature at ~37 oC ± 0.5oC → use heating elements (may have localized Electric Heating jacket overheating at low agitation speeds) → heated water (37oC) circulating around an outer “jacket”, or through coiled pipes within bioreactor → for small scale reactors may also use electric heating jacket that wraps around the vessel Heating probe that fits into a shaft that goes into the medium. Probe itself isn’t in contact with the medium. 1

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