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Strengths and Weakness
 

Strengths and Weakness

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Strengths and Weakness Strengths and Weakness Presentation Transcript

  • Strengths and weaknesses Waleed and Sarah
  • The Study
    • Original idea
    • GFP:
      • Readily detected
      • Not rejected by hosts
      • Expression sustained after cell division
      • Not expressed in RBC
      • Does it interfere with cellular interaction?
  • The rats!
    • Sample size
    • Bone chimeric rats: 7 weeks old
    • Tendon chimeric rats: 12 weeks old
    • Any difference in healing ability?
  • Bone chimeric rats
    • Proportion of GFP-positive cells
    • measured after transplantation.
    • Absence of GFP signal in other tissues
    • confirmed using laser SEM.
    • Why were 60 rats irradiated?
    • Low proportion of GFP-positive cells
    • after transplantation, and there was no
    • means of assessing host bone marrow
    • cells.
  • Tendon chimeric rats
    • Same tendon used.
    • Absence of GFP signals in
    • bone marrow and blood confirmed.
    • Histology and fluorescence of
    • patellar tendons observed to determine
    • survival of transplanted tendons.
    • Trauma of harvesting tendons from
    • GFP rats and adding nylon suture…
    • does it interfere with healing?
    • Only ≈65% GFP-positivity before injury
  • Methods
    • Left leg controls, confirmed GFP
    • positivity
    • Negative control: GFP-positivity
    • measured before injury.
    • Standardized
    • 7 days
    • Incision 1 wk after transplantation
    • in bone chimeric, but after 2 wks
    • in tendon chimeric.
  • Immunohistochemistry
    • Monoclonal antibody
    • Fixation and quick freezing
    • damages tendon
    • Control for BrdU
  • Tendon type
    • Only patellar used
    • What about intrasynovial tendons?
  • Subjective Analysis of Morphology
    • Lack of markers for MCS and fibroblasts
    • ‘Fibroblast like cells’-subjective
  • Functional Tissue Engineering
    • Managed to create constructs
    • that can withstand large forces
    • Forces measured in vivo using
    • transducers.
    • In vitro v.s. in vivo environments
    • effects on construct? Long-term?
    • How accurate are tensile strength measurements? Variables: direction
    • of forces, long term, in vivo conditions?
  • Final thoughts
    • Could there be other cells apart from fibroblasts involved?
    • Is it really biphasic?
    • Are rat tendons comparable to human?
  •