Group Members
•Adan Fatima Javed
•Sehrish Shoukat
•Zunaira Khalil
•Basharat Khan

•Syed Haider Ali
•Salimullah Khan Ayubi
Pesticides
Pesticides are substances meant for
preventing, destroying or mitigating
any pest.
Types of pesticides/Classes breakup

2%

Insecticides

9%
Herbicides

14%

Fungicides
75%
Acricides
Why Pesticides are used?
• Population pressure
• Food security
• Higher yield
• Province wise distribution

KPK 2%
Sindh 8%
Punjab
90%
Toxicity potential of Pesticides
Direct impact on humans

• Adversely impact on
Central Nervous
System (CNS)

• Toxic
Fate of Pesticide in Environment
Profenofos
Selected Pesticide-Profenofos

• Widely used in Pakistan
• Hazardous to health
Reasons
• Among top ten
used pesticides in
Pakistan
– Used on cotton
– Commonly found
as groundwater
contaminants
– More e...
Biodegradation
• Microorganisms
• Use compound as carbon and energy
source
Methodology
Methodology Steps
Sampling

Enrichment

Incubation

Spreading

Plate Count

Isolation

Acclimatization

Serial Dilutions

...
Media
Mineral Salt Media (MSM)
Use Distilled Water
For 100 ml of media

(0.15g/100 ml)
Autoclave
•Media
•Glass wares

e.g petri plates
etc….
Enrichment
Soil Sample
+
Media

Orbital Shaker
For 2 Days
Acclimatization
1 ml

Inoculum

Media

100 ml

100 ml

Concentration
of
profenofos

control

50 ppm

1 ml

100 ml

100 ppm...
Incubation
 For 2 days (48 hours)
at 37 oC.
Plate Count
Characterization
•Morphological Characterization

 Colony Morphology (Microscopy)
 Cell Morphology (Through staining)
•P...
Conclusion
•Specific growth of pesticide degrading microorganisms
•Planning for isolation and Characterization in the next...
Experience
•Very good experience
•Applications of Microbiology in Environmental
Engineering
•Would like to continue the re...
Facilities in IESE Microbiology Lab
•Sterile Environment
•Well equipped
•Very cooperative and trained Staff
•Students/Rese...
Suggestions
•

Separate lab for Bioremediation

• HEPA Filters
Acknowledgement




Professor Dr. Imran Hashmi
Miss Arish Naseem
Pesticides Degradation Through Microorganisms (Biodegradtion)
Pesticides Degradation Through Microorganisms (Biodegradtion)
Pesticides Degradation Through Microorganisms (Biodegradtion)
Pesticides Degradation Through Microorganisms (Biodegradtion)
Pesticides Degradation Through Microorganisms (Biodegradtion)
Pesticides Degradation Through Microorganisms (Biodegradtion)
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Pesticides Degradation Through Microorganisms (Biodegradtion)

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Pesticides Degradation Through Microorganisms (Biodegradtion)

  1. 1. Group Members •Adan Fatima Javed •Sehrish Shoukat •Zunaira Khalil •Basharat Khan •Syed Haider Ali •Salimullah Khan Ayubi
  2. 2. Pesticides Pesticides are substances meant for preventing, destroying or mitigating any pest.
  3. 3. Types of pesticides/Classes breakup 2% Insecticides 9% Herbicides 14% Fungicides 75% Acricides
  4. 4. Why Pesticides are used? • Population pressure • Food security • Higher yield
  5. 5. • Province wise distribution KPK 2% Sindh 8% Punjab 90%
  6. 6. Toxicity potential of Pesticides Direct impact on humans • Adversely impact on Central Nervous System (CNS) • Toxic
  7. 7. Fate of Pesticide in Environment
  8. 8. Profenofos
  9. 9. Selected Pesticide-Profenofos • Widely used in Pakistan • Hazardous to health
  10. 10. Reasons • Among top ten used pesticides in Pakistan – Used on cotton – Commonly found as groundwater contaminants – More effective
  11. 11. Biodegradation • Microorganisms • Use compound as carbon and energy source
  12. 12. Methodology
  13. 13. Methodology Steps Sampling Enrichment Incubation Spreading Plate Count Isolation Acclimatization Serial Dilutions Characterization
  14. 14. Media Mineral Salt Media (MSM) Use Distilled Water For 100 ml of media (0.15g/100 ml)
  15. 15. Autoclave •Media •Glass wares e.g petri plates etc….
  16. 16. Enrichment Soil Sample + Media Orbital Shaker For 2 Days
  17. 17. Acclimatization 1 ml Inoculum Media 100 ml 100 ml Concentration of profenofos control 50 ppm 1 ml 100 ml 100 ppm 1 ml 100 ml 150 ppm
  18. 18. Incubation  For 2 days (48 hours) at 37 oC.
  19. 19. Plate Count
  20. 20. Characterization •Morphological Characterization  Colony Morphology (Microscopy)  Cell Morphology (Through staining) •Physiological Characterization •Biochemical Characterization •Molecular Characterization
  21. 21. Conclusion •Specific growth of pesticide degrading microorganisms •Planning for isolation and Characterization in the next step
  22. 22. Experience •Very good experience •Applications of Microbiology in Environmental Engineering •Would like to continue the research in the next semesters
  23. 23. Facilities in IESE Microbiology Lab •Sterile Environment •Well equipped •Very cooperative and trained Staff •Students/Researchers are always welcomed
  24. 24. Suggestions • Separate lab for Bioremediation • HEPA Filters
  25. 25. Acknowledgement   Professor Dr. Imran Hashmi Miss Arish Naseem
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