Pesticides Degradation Through Microorganisms (Biodegradtion)
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Pesticides Degradation Through Microorganisms (Biodegradtion)

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Pesticides Degradation Through Microorganisms (Biodegradtion) Presentation Transcript

  • 1. Group Members •Adan Fatima Javed •Sehrish Shoukat •Zunaira Khalil •Basharat Khan •Syed Haider Ali •Salimullah Khan Ayubi
  • 2. Pesticides Pesticides are substances meant for preventing, destroying or mitigating any pest.
  • 3. Types of pesticides/Classes breakup 2% Insecticides 9% Herbicides 14% Fungicides 75% Acricides
  • 4. Why Pesticides are used? • Population pressure • Food security • Higher yield
  • 5. • Province wise distribution KPK 2% Sindh 8% Punjab 90%
  • 6. Toxicity potential of Pesticides Direct impact on humans • Adversely impact on Central Nervous System (CNS) • Toxic
  • 7. Fate of Pesticide in Environment
  • 8. Profenofos
  • 9. Selected Pesticide-Profenofos • Widely used in Pakistan • Hazardous to health
  • 10. Reasons • Among top ten used pesticides in Pakistan – Used on cotton – Commonly found as groundwater contaminants – More effective
  • 11. Biodegradation • Microorganisms • Use compound as carbon and energy source
  • 12. Methodology
  • 13. Methodology Steps Sampling Enrichment Incubation Spreading Plate Count Isolation Acclimatization Serial Dilutions Characterization
  • 14. Media Mineral Salt Media (MSM) Use Distilled Water For 100 ml of media (0.15g/100 ml)
  • 15. Autoclave •Media •Glass wares e.g petri plates etc….
  • 16. Enrichment Soil Sample + Media Orbital Shaker For 2 Days
  • 17. Acclimatization 1 ml Inoculum Media 100 ml 100 ml Concentration of profenofos control 50 ppm 1 ml 100 ml 100 ppm 1 ml 100 ml 150 ppm
  • 18. Incubation  For 2 days (48 hours) at 37 oC.
  • 19. Plate Count
  • 20. Characterization •Morphological Characterization  Colony Morphology (Microscopy)  Cell Morphology (Through staining) •Physiological Characterization •Biochemical Characterization •Molecular Characterization
  • 21. Conclusion •Specific growth of pesticide degrading microorganisms •Planning for isolation and Characterization in the next step
  • 22. Experience •Very good experience •Applications of Microbiology in Environmental Engineering •Would like to continue the research in the next semesters
  • 23. Facilities in IESE Microbiology Lab •Sterile Environment •Well equipped •Very cooperative and trained Staff •Students/Researchers are always welcomed
  • 24. Suggestions • Separate lab for Bioremediation • HEPA Filters
  • 25. Acknowledgement   Professor Dr. Imran Hashmi Miss Arish Naseem