Slide Presentation For Professor Jeff Hubblle (USA)

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09th March 2011

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Slide Presentation For Professor Jeff Hubblle (USA)

  1. 1. Development of an ex-vivo three-dimensional model of Morphology of porous structure using scanning electron microscopy (SEM) chronic lymphocytic leukaemia (CLL) by using biopolymers (PHAs) PHB 4% (w/v) PHB 4% (w/v) - EnlargedNovelty: Be able to fabricate porous 3-D scaffolds with an improved thickness of > 4 mm from PHB and PHBV and to model the ex-vivo 3-D abnormal BM niches Solvent evaporationPolymer solution Porogen + DIW (Complied with UK-SED,in organic solvent Polymer leaching 2002: <20 mg/m3) solution + Porogen PHBV 4% (w/v) PHBV 4% (w/v) - Enlarged 2 3 4 Porous 3-D 5 scaffolds 1 + Polymer + Polymer + Solvent + Porogen cast Porogen castPorogen (i.e., NaCl, Rectangular size ofsucrose etc.) polymeric porous 3-D 6 scaffolds (> 4mm) Physical properties of polymeric porous 3-D scaffolds Solvent-Casting Particulate LeachingMorphology of porous 3-D scaffolds of 4% (w/v) PHB 4% (w/v) PHBV 4% (w/v) morphology of porous 3-D scaffolds of 4% (w/v) Macro-indentation test of polymeric porous 3-D scaffolds INNER SIDE INNER SIDE PHBV 4% (w/v) Both polymers have a good mechanical strength and highly flexible materials as compared with porous PHB 4% (w/v) ∼10 mm biodegradable composites & porous biodegradable ∼10 mm polymers ∼5 mm PU 3-D foams - Laleh et al. Langmuir, 2006 INNER INNER SIDE SIDE
  2. 2. Physical properties pre-Surface treatment of PHB and PHBV porous 3-D and post surface treatmentscaffolds with an improved thickness to enhance Surface roughness was found to be significantlycell biomaterial adhesion and interactions affected by the treatment except porosity which remained quite similar Water contact angle post treatment with more voids were developed where otherwise covered by the thin films on the main pores In vitro degradation study of polymeric porous 3-D scaffolds in growth media Morphology of porous structure using scanning electron microscopy (SEM) 0.4 M NaOH PHB 0.4 M NaOH PHBV 0.6 M NaOH PHB 0.6 M NaOH PHBV Effect of surface treatments on cell-biomaterial affinity of polymeric porous 3-D scaffold Type of cell: Human chronic lymphocytic leukemia cell line (RL) All untreated and treated polymeric foams were comparatively PHB rf-oxygen plasma PHBV rf-oxygen plasma similar with all of them exhibited high cell number after 14 days

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