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  • 1. Detection of Pristine Gas Two Billion Years After the Big Bang Michele Fumagalli, et al. Science 334, 1245 (2011); DOI: 10.1126/science.1213581 This copy is for your personal, non-commercial use only. If you wish to distribute this article to others, you can order high-quality copies for your colleagues, clients, or customers by clicking here. Permission to republish or repurpose articles or portions of articles can be obtained by following the guidelines here. The following resources related to this article are available online at Downloaded from on December 1, 2011 (this infomation is current as of December 1, 2011 ): Updated information and services, including high-resolution figures, can be found in the online version of this article at: Supporting Online Material can be found at: This article appears in the following subject collections: Astronomy (print ISSN 0036-8075; online ISSN 1095-9203) is published weekly, except the last week in December, by theAmerican Association for the Advancement of Science, 1200 New York Avenue NW, Washington, DC 20005. Copyright2011 by the American Association for the Advancement of Science; all rights reserved. The title Science is aregistered trademark of AAAS.
  • 2. RESEARCH ARTICLES PICALM, as well as two genes whose protein References and Notes 32. L. Bertram et al., Am. J. Hum. Genet. 83, 623products (BIN1 and CD2AP) interact with hits 1. V. Khurana, S. Lindquist, Nat. Rev. Neurosci. 11, 436 (2008). (2010). 33. D. Blacker et al., Neurology 48, 139from our screen, are AD risk factors. Given the 2. T. F. Outeiro, S. Lindquist, Science 302, 1772 (1997).diversity of pathologies, however, their connec- (2003). 34. D. A. Bennett, P. L. De Jager, S. E. Leurgans,tion to Ab toxicity was unknown. Our work in 3. N. Thayanidhi et al., Mol. Biol. Cell 21, 1850 J. A. Schneider, Neurology 72, 1495 (2009).yeast, nematodes, and rat cortical neurons clearly (2010). 35. J. M. Shulman et al., PLoS ONE 5, e11244 (2010). 4. A. R. Winslow et al., J. Cell Biol. 190, 1023 (2010). 36. C. D. Link, Proc. Natl. Acad. Sci. U.S.A. 92, 9368places these factors within the Ab cascade, link- 5. A. D. Gitler et al., Nat. Genet. 41, 308 (2009). (1995).ing Ab to the genetics of sporadic AD. 6. L. J. Su et al., Dis Model Mech 3, 194 (2010). 37. D. Bozyczko-Coyne et al., J. Neurochem. 77, 849 Neurons are particularly vulnerable to per- 7. A. A. Cooper et al., Science 313, 324 (2006). (2001).turbations in the homeostasis of endocytosis, 8. J. Hardy, D. J. Selkoe, Science 297, 353 (2002). 38. Y. Sun, S. Carroll, M. Kaksonen, J. Y. Toshima, 9. M. Knobloch, U. Konietzko, D. C. Krebs, R. M. Nitsch, D. G. Drubin, J. Cell Biol. 177, 355 (2007).because they must constantly recycle both neuro- 39. L. Maldonado-Báez et al., Mol. Biol. Cell 19, 2936 Neurobiol. Aging 28, 1297 (2007).transmitters and their receptors (42). Ab interacts 10. E. B. Lee et al., J. Biol. Chem. 281, 4292 (2006). (2008).with, and alters signaling by, a variety of neuronal 11. G. M. Shankar et al., Nat. Med. 14, 837 (2008). 40. A. J. Miñano-Molina et al., J. Biol. Chem. 286, 27311receptors (43). We propose that the conforma- 12. D. M. Walsh, B. P. Tseng, R. E. Rydel, M. B. Podlisny, (2011). D. J. Selkoe, Biochemistry 39, 10831 (2000). 41. L. Qiang et al., Cell 146, 359 (2011).tional flexibility of these oligomers allows them 13. R. Kayed et al., Science 300, 486 (2003). 42. N. Jung, V. Haucke, Traffic 8, 1129 (2007).to interact rather promiscuously with conforma- 14. J. Shorter, S. Lindquist, Science 304, 1793 (2004). 43. Y. Verdier, M. Zarándi, B. Penke, J. Pept. Sci. 10, 229tionally flexible unliganded receptors, which, in 15. F. M. LaFerla, Biochem. Soc. Trans. 38, 993 (2004).turn, disrupts endocytic homeostasis. (2010). Downloaded from on December 1, 2011 Our yeast screen also identified seven con- 16. L. M. Ittner et al., Cell 142, 387 (2010). Acknowledgments: We thank L. Chibnik, B. Keenan, and 17. E. D. Roberson et al., Science 316, 750 (2007). D. Ng for helpful discussions; D. Wittrup, V. Lee, M. Vidal,served genes functionally associated with the 18. G. Thinakaran, E. H. Koo, J. Biol. Chem. 283, 29615 and C. Link for materials; and J. Corneveaux, M. Huentelman,cytoskeleton. Because yeasts do not express tau, (2008). and other Translational Genomics investigators forour findings may indicate that the connection be- 19. D. J. Selkoe, M. S. Wolfe, Cell 131, 215 (2007). assistance with the human study cohorts and participantstween Ab toxicity and the cytoskeleton is more 20. H. R. Pelham, Annu. Rev. Cell Biol. 5, 1 (1989). in the Religious Orders Study and the Rush Memory and Aging 21. J. M. Kowalski, R. N. Parekh, K. D. Wittrup, Biochemistry Project. This work was supported by a Howard Hughes Medicaldeeply rooted than tau alone, probably involv- 37, 1264 (1998). Institute Collaborative Innovation Award; National Researching clathrin-mediated endocytosis. In analyzing 22. M. Lindhagen-Persson, K. Brännström, M. Vestling, Service Award fellowship F32 NS067782-02; the Curehuman GWAS data we also uncovered sugges- M. Steinitz, A. Olofsson, PLoS ONE 5, e13928 Alzheimer’s Fund; NIH grants K08AG034290, P30AG10161,tive associations between AD and three other (2010). R01AG15819, and R01AG17917; the Kempe foundation; and Alzheimerfonden. This paper is dedicated to Nell Matheney,genes—XPO1, ADSSL1, and RABGEF1—and 23. Supporting material is available on Science Online. Zhao Xindi, Rochelle Glatt, Matyas Topolszki, their 24. S. D. Stamenova, R. Dunn, A. S. Adler, L. Hicke,confirmed their Ab relationships in yeast and J. Biol. Chem. 279, 16017 (2004). families, and the countless other victims of Alzheimer’snematode. 25. D. Harold et al., Nat. Genet. 41, 1088 (2009). disease. The treatments available for AD are few and 26. J. C. Lambert et al.; European Alzheimer’s Diseasetheir efficacy limited. Determining how best to Initiative Investigators, Nat. Genet. 41, 1094 (2009). 27. S. Seshadri et al., JAMA 303, 1832 (2010). Supporting Online Materialrescue neuronal function in the context of the 28. A. R. Ramjaun, K. D. Micheva, I. Bouchelet, P. S. McPherson, brain is a problem of staggering proportions. J. Biol. Chem. 272, 16700 (1997). Materials and Methods Figs. S1 to S7Our yeast model provides a tool for identifying 29. G. Gaidos, S. Soni, D. J. Oswald, P. A. Toselli, K. H. Kirsch, Tables S1 to S7genetic leads, investigating their mechanisms of J. Cell Sci. 120, 2366 (2007). 30. P. Hollingworth et al.; Alzheimer’s Disease Neuroimaging References (44–57)action, and screening for genetic and small-molecule Initiative; CHARGE consortium; EADI1 consortium, 26 August 2011; accepted 18 October 2011modifiers of this devastating and etiologically com- Nat. Genet. 43, 429 (2011). Published online 27 October 2011;plex disease. 31. A. C. Naj et al., Nat. Genet. 43, 436 (2011). 10.1126/science.1213210Detection of Pristine Gas Two Billion Wb,0). BBN theory also predicts that there was negligible production of the heavy elements with abundance ratios X/H < 10−10, becauseYears After the Big Bang the physical conditions now typical of stellar interiors did not yet exist (1). The analysis of gas observed in absorptionMichele Fumagalli,1* John M. OMeara,2 J. Xavier Prochaska3 along the lines of sight to high-redshift quasars, distant galaxies that host supermassive blackIn the current cosmological model, only the three lightest elements were created in the first few holes, is a powerful probe of the BBN yields.minutes after the Big Bang; all other elements were produced later in stars. To date, however, Particular attention has been given to D, partlyheavy elements have been observed in all astrophysical environments. We report the detection of because of observational convenience, but alsotwo gas clouds with no discernible elements heavier than hydrogen. These systems exhibit the because the D/H abundance ratio is very sensi-lowest heavy-element abundance in the early universe, and thus are potential fuel for the most tive to Wb,0. For quasar sight lines, the measuredmetal-poor halo stars. The detection of deuterium in one system at the level predicted by log(D/H) = − 4.55 T 0.03 (2, 3) translates intoprimordial nucleosynthesis provides a direct confirmation of the standard cosmological model. Wb,0h2(BBN) = 0.0213 T 0.0010, which is fullyThe composition of these clouds further implies that the transport of heavy elements from consistent with the value inferred from the cosmicgalaxies to their surroundings is highly inhomogeneous. 1 Department of Astronomy and Astrophysics, University of n modern cosmological theory, the light ele- this brief epoch, termed Big Bang nucleosynthe- California, Santa Cruz, CA, USA. 2Department of Chemistry andI ments and their isotopes were produced dur- ing the first few minutes after the Big Bang,when the universe cooled during expansion from sis (BBN), deuterium (D), 3He, 4He, and 7Li were synthesized with an abundance ratio relative to hydrogen (H) that was sensitive to the cosmic Physics, Saint Michaels College, Colchester, VT, USA. 3Uni- versity of California Observatories–Lick Observatory, University of California, Santa Cruz, CA, USA. *To whom correspondence should be addressed. E-mail:temperatures T ∼ 109 K to below ∼4 × 108 K. In density of ordinary matter (i.e., the baryon density SCIENCE VOL 334 2 DECEMBER 2011 1245
  • 3. RESEARCH ARTICLES Fig. 1. Peak-to-peak variation of the observed metallicity in z ≳ 2 cosmic ρ/ρmean structures at different densities. The blue, green, and red rectangles show the 100 105 1010 1015 102 spread in observed metallicities for diffuse gas in the universe; respectively, Quasars these are the IGM, LLSs, and galactic ISM. Orange rectangle: H II regions in galaxies. Light blue rectangle: quasar broad-line regions. The black point with LLSs error bars marks the mean metallicity and the standard deviation for z > 1.5 HII regions Halo stars 100 ISM LLSs. Galactic halo stars (stars connected with a dashed line) are represented at IGM arbitrary density for visualization purposes. The top axis translates the number density in the overdensity above the mean baryon cosmic density at z = 3.5. The Z(Z ) upper limits on the metallicity for LLS1134a and LLS0956B are shown with green arrows, assuming logU = −3. Higher ionization parameters would shift 10-2 these limits to lower densities and lower metallicity. See SOM text 5 for additional details on the observations presented in this figure. 10-4 LLS0956B LLS1134a 10-5 100 105 1010 Downloaded from on December 1, 2011 Number density (cm-3) Fig. 2. (Top) Keck/LRIS spectrum of the quasi-stellar object 40 (QSO) J1134+5742. A LLS at z ∼ 3.4 is clearly visible from Relative Flux the break at ∼4000 Å. (Middle) H I Lyman series transi- 30 tions in LLS1134a. Superimposed on the data are the best- fit model (red line) and the 2s errors (yellow shaded regions). 20 Individual components included in the model are marked with thin gray lines, and the positions of the HI and DI are 10 indicated by vertical dotted lines. The second HI component 0 (LLS1134b) at +54 km s−1 is marked with a dash-dotted line. (Bottom) Selected strong metal-line transitions in LLS1134a 3500 4000 4500 5000 5500 (dotted lines) and LLS1134b (dash-dotted lines). Unrelated Wavelength (Å) absorption from the IGM contaminates the Si III transition (fig. S4). 1.2 α 1.0 0.8 0.6 0.4 0.2 0.0 −100 −50 0 50 100 −50 0 50 100 1.2 Normalized Flux 1.0 0.8 0.6 1.2 1.0 0.8 0.6 −40 −20 0 20 40 60 −40 −20 0 20 40 60 Velocity (km s−1)1246 2 DECEMBER 2011 VOL 334 SCIENCE
  • 4. RESEARCH ARTICLES 2.0 microwave background (CMB) power spectrum Normalized Flux Wb,0h2(CMB) = 0.02249+0.00056− 0.00057 [from 1.5 7-year Wilkinson microwave anisotropy probe (WMAP7) data (4)]. This excellent agreement 1.0 between two essentially independent experi- 0.5 ments stands as a marked triumph of the Big Bang theory. 0.0 On the other hand, all of the systems with −0.5 measured D have heavy-element abundances that 3740 3760 3780 3800 3820 3840 3860 3880 exceed, by many orders of magnitude, the BBN Wavelength (Å) prediction. In fact, despite the measurement of thousands of galaxies from the early universe (5, 6) and careful study of the diffuse gas that permeates the universe (7, 8), one has yet to 1.0 detect anything near primordial enrichment. For structures denser than the mean cosmic density (Fig. 1), the high-redshift universe has exhibited 0.5 a floor in the metallicity Z (the mass fraction of Downloaded from on December 1, 2011 elements heavier than H), at ∼1/1000 of the so- lar abundance (Z⊙). Similarly, among several old 0.0 and iron-poor stars, only one has a metallicity −50 0 50 −100 −50 0 50 100 Z ∼ 10−4 Z⊙ (9), with all the remaining stars 1.4 having enhanced C or O abundances. The exis- Normalized Flux tence of a minimum level of enrichment at about 1.2 1/1000 of solar has been associated with the metal production in Population III (PopIII) stars, 1.0 which are primordial stars that form in metal-free environments via H2 cooling. In fact, models and 0.8 numerical simulations (10, 11) show that ejecta from this first stellar population could have en- riched the interstellar medium (ISM) of the host 1.4 halos up to ∼10−3 Z⊙, and polluted the surround- ing intergalactic medium (IGM) as soon as 1.2 1 billion years after the Big Bang (redshift z ∼ 6). In this work, we report on the H and metal 1.0 properties of two gas “clouds” at z ∼ 3, when the universe was only two billion years old. We 0.8 observed quasars SDSS J113418.96+574204.6 (emission redshift zem = 3.522) and Q0956+122 (zem = 3.297) on UT 3 and 5 January 2006 and −40 −20 0 20 40 −40 −20 0 20 40 UT 7 April 2006 with the High Resolution Velocity (km s−1) Echelle Spectrometer HIRES on the Keck I tele- scope on Mauna Kea [supporting online mate-Fig. 3. (Top) Keck/HIRES spectrum of the QSO Q0956+122. The flux decrements at ∼3860 and ∼3750 Å rial (SOM) text 1]. Previous low-resolution spectrareveal two partial LLSs at z ∼ 3.22 (LLS0956A) and z ∼ 3.10 (LLS0956B). The corresponding Lyman of these quasars from the Sloan Digital Sky Sur-limits are marked with vertical lines. (Middle) HI Lyman series transitions for LLS0956B shown relative vey (SDSS) had shown significant absorptionto z = 3.096221. Superimposed on the data are the best-fit model (red lines) and 2s uncertainties at wavelengths l < 4000 Å characteristic of sub-(yellow shaded regions). (Bottom) Selected strong metal-line transitions for LLS0956B. stantial optical depth tLL at the H I Lyman limit (at wavelength l < 912 Å), typical of the LymanTable 1. Summary of the physical properties of LLS1134a and LLS0956B. For each system, we present limit systems (LLSs).redshift, the HI column density (NHI), the D abundance (D/H), the Doppler parameter (bHI), the temperature, Our Keck/HIRES spectrum of J1134+5742the metallicity, the H neutral fraction (xHI), the total H volume density (nH), and the ionization parameter (U). reveals a sharp break in the flux at ∼4000 Å, indicating the presence of a LLS at z ∼ 3.4 with LLS1134a LLS0596B tLL > 2 (hereafter named LLS1134; Fig. 2), con-Redshift 3.410883 T 0.000004 3.096221 T 0.000009 firming the lower-resolution data. A search forlogNHI 17.95 T 0.05 17.18 T 0.04 absorption lines at a redshift consistent with thelogD/H −4.69 T 0.13* – Lyman break further reveals the presence of thebHI (km s−1) 15.4 T 0.3 20.2 T 0.8 H I Lyman series through to Lyman-22 (H ITemperature (K) < (1.43 T 0.05) × 104 <(2.48 T 0.19) × 104 913.5), corresponding to l ∼ 4030 Å. In the high-Metallicity ( Zʘ) <10−4.2 <10−3.8 resolution spectrum, two distinct absorbers canlogxHI ≤2.10 ≤2.40 be identified within LLS1134, the main systemlognH ≤1.86 ≤1.98 (LLS1134a) at z = 3.410883 and a weaker com-logU† ≥3 ≥3 ponent (LLS1134b) at z = 3.41167, separated by*Including LLS1134a, the current best estimate for the primordial D abundance becomes ¯¯¯¯¯¯¯ = − 4.556 T 0.034. log(D/H) a velocity difference dv ∼ 54 km s−1. In contrast,†The listed values are physically motivated but not directly measured. The metallicity, xHI, and nH depend on the assumed value. two flux decrements in the normalized HIRES SCIENCE VOL 334 2 DECEMBER 2011 1247
  • 5. RESEARCH ARTICLES spectrum of Q0956+122 (Fig. 3) are visible at of metals, have total heavy-element abundance The detection of D in the metal-free LLS1134a ∼3860 and ∼3750 Å, revealing the presence of comparable to these limits, but generally above provides a direct confirmation of the BBN. From two LLSs with tLL ∼ 1 at z ∼ 3.22 (LLS0956A) ∼1/1000 of solar. Our analysis uncovers regions the analysis of the D and H absorption lines, we and z ∼ 3.10 (LLS0956B). of the universe at z < 6 with essentially primor- derive log(D/H) = −4.69 T 0.13 (SOM text 2). The A closer inspection of these spectra also re- dial enrichment, whose traces can be found observed value is consistent at 1s with the the- veals no detectable metal-line absorption at the within the oldest stellar populations in the present oretical predicted value log(D/H) = −4.592 (15), velocity of the strongest H I component for either universe (9). assuming Wb,0 from WMAP7. This measurement LLS1134a or LLS0956B. Such a complete ab- sence of heavy-element absorption has not been previously reported from data with comparable Fig. 4. D abundances −4.3 sensitivity. For LLS1134a, besides the H I Lyman as a function of metal- NHI < 10 20 cm−2 series, the only other detected transitions are licity [(X/H) ≡ log(X/H) − NHI > 10 20 cm−2 log(X/H)ʘ] for the z > 2 −4.4 [Si/H] D I Lya and Lyb, offset by the appropriate dv = [O/H] 0.018 −82 km s−1 from the H absorption. absorption-line systems. To characterize the physical properties of Green symbols are for −4.5 these LLSs (summarized in Table 1), we modeled LLSs; red symbols are for the H and D absorptions using a c2 minimization higher–column-density log (D/H) absorbers (NHI > 1020 0.022 algorithm (table S1; figs. S2 and S3; and SOM −4.6 Ωb,0 h2 cm−2). Metallicities ob- Downloaded from on December 1, 2011 text 2), and we derived upper limits on the column tained with Si are indi- densities of various ionization states of heavy 0.026 cated by squares, whereas −4.7 elements (table S2; fig. S4; and SOM text 3). those obtained with O To translate these limits into constraints on the are indicated with circles. gas metallicity, it is necessary to make assump- The right-hand axis trans- −4.8 0.030 tions about the ionization state of the gas (SOM lates the D abundance to text 4). Every LLS with HI column density NHI ≲ the cosmic baryon den- 0.034 1019 cm−2 analyzed to date has exhibited absorp- sity Ωb,0h2. The inferred −4.9 −4.5 −4.0 −3.5 −3.0 −2.5 −2.0 −1.5 tion characteristic of a predominantly ionized gas Ωb,0h (BBN) is shown 2 Metallicity ([X/H]) (12, 13). The standard interpretation is that the with a solid blue line, to- medium has been photoionized by an external gether with the 1s errors (light blue shaded area). The CMB value and 1s errors from WMAP7 are shown radiation field, presumably a combination of the with a solid black line and a gray dashed area, respectively. extragalactic ultraviolet background (EUVB) gen- erated by the cosmological population of quasars and galaxies, together with emission from local 0 10 PopIII/PopII transition sources (such as a nearby galaxy). This conclu- IGM(PopIII) sion is based on comparison of the observed ionic Halos(PopIII+PopII) column densities of heavy elements with simple ISM(PopII) ISM/IGM(PopII) photoionization models. Every previously ana- 10 -1 Gas(PopII) lyzed LLS has shown substantial absorption from doubly and triply ionized species, such as Si ++ and C +3, which trace predominantly ionized gas. -2 Parametrizing the ionization state in terms of the 10 ionization parameter U ≡ F/(cnH), with F the Z(Z ) flux of ionizing photons and nH the gas volume density, all previously analyzed LLSs have ex- 10 -3 hibited U ≥ 10−3, corresponding to nH ≤ 10−2 cm−3 at z ∼ 3 (fig. S6). Adopting this U value as a limit to the ioniza- -4 tion state and current estimates for the spectral 10 shape of the EUVB (14), we infer metallicities of Z < 10−4.2 Z⊙ and Z < 10−3.8 Z⊙ for LLS1134a and LLS0956B (fig. S5). These upper limits are 10 -5 100 to 1000 times lower than typical measure- ments of LLSs and are over an order of magnitude 0 5 10 15 20 lower than any previous observed metallicity Redshift at z > 2 (Fig. 1). These limits are only comparable Fig. 5. Overview of analytic models and simulations for the metal enrichment of the universe. Light blue- to the abundances detected in the most metal- and green-shaded regions: IGM metallicity from PopIII stars with mixing between 1 and 0.05 and poor star (9) and are suggestive of a primordial different star formation histories (10, 21). Red dashed region: Critical metallicity that marks the composition. Supersolar metallicity is commonly transition between PopIII and PopII stars (23, 33). Orange line: Analytic model for the gas metal content found in the surroundings of quasars as well as in the universe from PopII stars and galactic winds (20). Gray-shaded region: Gas metallicity within halos in a few LLSs. Metallicities between solar and from hydrodynamical simulations that include yields from both PopIII and PopII stars (22). Blue-shaded ∼1/10 of solar are typical of galaxies, and sub- region: Analytic model for the ISM metallicity at different halo masses (1011 to 1014 Mʘ) and different solar metal enrichment down to 10−3 Z⊙ is char- wind models (34). Black lines: Metallicity from hydrodynamical simulations with momentum-driven acteristic of the ISM and IGM at early epochs. winds (19) in condensed gas (solid line), hot halo (dotted line), warm-hot intergalactic medium (dashed The most iron-poor stars in the Galactic halo, line), diffuse gas (dash-dotted line), ISM (dash–triple-dotted line), and stars (long-dashed line). Upper thought to be the repository of the first generation limits on the metallicities of LLS1134a and LLS0956B are marked with green arrows.1248 2 DECEMBER 2011 VOL 334 SCIENCE
  • 6. RESEARCH ARTICLESis in agreement with previous determinations potentially give rise to PopIII stars two billion 9. E. Caffau et al., Nature 477, 67 (2011).in quasar absorption-line systems at z > 2 (table years after the transition between the first and 10. J. Mackey, V. Bromm, L. Hernquist, Astrophys. J. 586, 1 (2003).S3 and Fig. 4). Because the lack of metals in second generation of stars (PopII) is thought to 11. J. H. Wise, T. Abel, Astrophys. J. 685, 40 (2008).LLS1134a confirms its pristine composition, this have occurred. Thus, pair-production supernovae 12. G. E. Prochter, J. X. Prochaska, J. M. OMeara, S. Burles,agreement strengthens the hypothesis that at associated with the death of these massive and R. A. Bernstein, Astrophys. J. 708, 1221 (2010).low metallicities (10−2 Z⊙ or less), the observed D metal-free stars may be found even at modest 13. J. X. Prochaska, S. M. Burles, Astron. J. 117, 1957 (1999).abundances are representative of the primordial redshifts (24). 14. F. Haardt, P. Madau, (2011).value (16), and astration cannot be responsible The pristine composition of LLS1134a and 15. G. Steigman, Annu. Rev. Nuclear Particle Sci. 57, 463for the lingering scatter in the observed D/H. D LLS0956B can be reconciled with model pre- (2007).abundances from quasar absorption-line sys- dictions and previous observations if mixing of 16. D. Kirkman, D. Tytler, N. Suzuki, J. M. OMeara, D. Lubin,tems are therefore solid anchor points for mod- metals within the IGM is an inefficient and in- Astrophys. J. Suppl. Ser. 149, 1 (2003). 17. D. Romano, M. Tosi, C. Chiappini, F. Matteucci, Mon. Not.els of galactic chemical evolution (17). Combining homogeneous process. A varying degree of metal R. Astron. Soc. 369, 295 (2006).D/H in LLS1134a with values from the known enrichment is seen in multiple components of 18. K. Kohler, N. Y. Gnedin, Astrophys. J. 655, 685D-bearing systems, we obtain a logarithmic LLSs (12, 13, 25), implying that mixing does not (2007).weighted mean ¯¯¯¯¯¯¯¯ ¼ − 4:556 T 0:034 logðD=HÞ operate effectively on small scales. The detection 19. B. D. Oppenheimer, R. Davé, N. Katz, J. A. Kollmeier, D. H. Weinberg, (2011).that translates into Wb,0h2(BBN) = 0.0213 T of ionized metals in LLS1134b, the weaker com- 20. L. Hernquist, V. Springel, Mon. Not. R. Astron. Soc. 341,0.0012, after accounting for both random and ponent at +54 km s−1 from LLS1134a, reinforces 1253 (2003).systematic errors (3). Consistent with previous this point. Further, studies of metal systems in the 21. N. Yoshida, V. Bromm, L. Hernquist, Astrophys. J. 605, Downloaded from on December 1, 2011studies, we do not include in the weighted mean low-density and diffuse IGM suggest that at least 579 (2004). 22. J. H. Wise, M. J. Turk, M. L. Norman, T. Abel,the error on the assumed level of the quasar some of the ionized heavy elements (such as C (2010).continuum light. IV) are in small and short-lived clumps (26). A 23. V. Bromm, A. Ferrara, P. S. Coppi, R. B. Larson, Mon. Not. The absence of metals in LLS1134a and low volume-filling factor for metals is also con- R. Astron. Soc. 328, 969 (2001).LLS0956B is also unusual in the framework of sistent with theories of metal ejection from su- 24. E. Scannapieco, P. Madau, S. Woosley, A. Heger,theories for the metal enrichment of cosmic struc- pernovae, in which most of the heavy elements A. Ferrara, Astrophys. J. 633, 1031 (2005). 25. V. DOdorico, P. Petitjean, Astron. Astrophys. 370, 729tures (Fig. 5). Numerical simulations suggest that are initially confined in small bubbles (27) and (2001).LLSs typically arise in galaxies (18) in dense only subsequently diffuse in the surrounding IGM. 26. J. Schaye, R. F. Carswell, T.-S. Kim, Mon. Not. R. Astron.gas above ∼100 times the mean cosmic density Plausibly, LLS1134a and LLS0956B originated Soc. 379, 1169 (2007).rmean. At the same time, in models of the IGM in a filament of the cosmic web where primor- 27. A. Ferrara, M. Pettini, Y. Shchekinov, Mon. Not. R. Astron. Soc. 319, 539 (2000).enrichment, metals are ejected to hundreds of dial regions coexisted with enriched pockets of 28. D. Keres, N. Katz, D. H. Weinberg, R. Davé, Mon. Not. R.kiloparsecs from star-forming regions, resulting gas. If metal enrichment is highly inhomoge- Astron. Soc. 363, 2 (2005).in substantial pollution of the nearby gas. As a neous, these two LLSs could just be the tip of 29. A. Dekel et al., Nature 457, 451 (2009).consequence, the metallicity predicted for stars or the iceberg of a much larger population of un- 30. C.-A. Faucher-Giguère, D. Keres, Mon. Not. R. Astron. Soc. 412, L118 (2011).for the ISM at z < 4 (19) ranges between 0.1 and polluted absorbers that trace a large fraction of 31. M. Fumagalli et al., Mon. Not. R. Astron. Soc., 10.1111/1 Z⊙, which is three orders of magnitude higher the dense IGM. j.1365-2966.2011.19599.x (2011).than the limits inferred for these two LLSs. Sim- Besides the implications for BBN and metal 32. F. van de Voort, J. Schaye, G. Altay, T. Theuns,ilarly, the metallicity predicted for the hot halo of distribution, the detection of metal-free LLSs is (2011). 33. R. Schneider, A. Ferrara, P. Natarajan, K. Omukai,galaxies and for the surrounding IGM (19, 20) tantalizing in the context of galaxy formation and Astrophys. J. 571, 30 (2002).exceeds by a factor of 10 or more the limits of evolution. Modern theory and simulations predict 34. R. Davé, K. Finlator, B. D. Oppenheimer,LLS1134a and LLS0956B. Contrary to any predic- that most of the gas that sustains star formation (2011).tion and any previous observation, these two LLSs is accreted in galaxies through dense and narrowreside at significant overdensity (r/rmean < 850), streams, known as cold flows (28, 29). These Acknowledgments: We thank A. Aguirre, N. Lehner, and P. Madau for providing comments on this manuscript.but in an unpolluted portion of the universe. gaseous filaments are highly ionized by both We thank the Max-Planck-Institut für Astronomie at Metallicity below 10−4 Z⊙ appears excep- the EUVB and the radiation escaping from the Heidelberg for their hospitality. J.X.P. acknowledgestional even when compared to the level of pre- central star-forming regions. Cold flows should support from the Humboldt Foundation. Support forenrichment from PopIII stars that is predicted by therefore appear as LLSs in the spectra of bright this work came from NSF grant AST0548180. We acknowledge the use of the VPFIT program. This workmodels (10, 21, 22) only 500 million years after quasars (30–32). Further, in contrast to metal- is based on observations made at the W. M. Keckthe Big Bang (z ∼ 10). Our limits place addi- enriched gas that is outflowing from galaxies, this Observatory, which is operated as a scientific partnershiptional constraints on the widespread dispersal of infalling material is expected to be metal-poor among the California Institute of Technology, themetals from primordial stellar populations and (31). Although direct observational evidence of University of California, and NASA. The observatory was made possible by the generous financial supportthe first generations of galaxies. The gas we de- cold flows is still lacking, these streams are thought of the W. M. Keck Foundation. The authors recognizetected could in principle fuel PopIII star forma- to be ubiquitous at high redshift, and primordial and acknowledge the very significant cultural role andtion at z ∼ 3, because its metallicity lies at or even LLSs such as LLS1134a and LLS0956B are ideal reverence that the summit of Mauna Kea has alwaysbelow the minimum enrichment required for candidates for this elusive mode of accretion. had within the indigenous Hawaiian community. We are most fortunate to have the opportunity to conductmetal cooling to induce fragmentation in the observations from this mountain. The data reportedcollapsing material in the absence of dust (23). References and Notes in this paper are available through the Keck ObservatoryGiven a lower limit on the LLS physical size l = 1. E. M. Burbidge, G. R. Burbidge, W. A. Fowler, F. Hoyle, Archive.NHI/(xHInH), with xHI the neutral fraction and Rev. Mod. Phys. 29, 547 (1957). 2. J. M. OMeara et al., Astrophys. J. 649, L61 (2006).nH the total H volume density, we can infer the 3. M. Pettini, B. J. Zych, M. T. Murphy, A. Lewis, C. C. Steidel, Supporting Online Materialtotal H mass in these clouds M H = mpNHl2. Mon. Not. R. Astron. Soc. 391, 1499 (2008). find MH ≳ 6.2 × 106 M⊙ for LLS1134a and 4. E. Komatsu et al., Astrophys. J. Suppl. Ser. 192, 18 (2011). SOM Text Figs. S1 to S6MH ≳ 4.2 × 105 M⊙ for LLS0956B, which is 5. J. X. Prochaska, E. Gawiser, A. M. Wolfe, S. Castro, Tables S1 to S3comparable to the mass of the mini-halos where S. G. Djorgovski, Astrophys. J. 595, L9 (2003). 6. D. K. Erb et al., Astrophys. J. 644, 813 (2006). References (35–68)the first generation of stars formed (22). There- 7. J. Schaye et al., Astrophys. J. 596, 768 (2003). 5 September 2011; accepted 31 October 2011fore, if this gas were able to collapse further and 8. R. A. Simcoe, W. L. W. Sargent, M. Rauch, Astrophys. J. Published online 10 November 2011;shield from the ambient UV radiation, it would 606, 92 (2004). 10.1126/science.1213581 SCIENCE VOL 334 2 DECEMBER 2011 1249