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Splenocyte isolation

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Transcript

  • 1. Splenocyte Isolation
  • 2. Splenocyte IsolationPurpose: To define and understand the lymphatic system and its components, and to purify and culture splenocytes.
  • 3. Splenocyte: any one of the different white bloodcell types that are situated in the spleen orpurified from splenic tissue.Splenocytes generally consist of phagocyticmacrophages, antigen-presenting cells, andmature T and B lymphocytes.
  • 4. The lymphoid system orlymphatic system: consists of all of the tissue aggregates and organs composed of lymphoidtissue which function together to produce specific resistance to disease (immunity).
  • 5. Primary lymphoid organs Secondary lymphoid organs site of lymphopoiesis provide interaction of acquire Ag receptors lymphocytes with themselves,selected for tolerance to self-Ags accessory cells, and Ag Ex: Thymus (T) and fetal liver disseminate immune responses and bone marrow (B) Ex: Lymph nodes and the spleen
  • 6. fetalliver
  • 7. Disseminate: to distribute or spread something,especially information, or become widespread.Used within this context, secondary lymphoidorgans are capable of dispatching the appropriateWBCs to the site of infection.
  • 8. Lymph nodes and the spleen are the most highlyorganized of the secondary lymphoid organs.Lymph nodes: the sites where immune responsesare mounted to antigens in lymph.The spleen: a secondary lymphoid organ thatspecializes in filtering blood and trapping blood-borne antigens.
  • 9. Lymph nodes Spleenresponses mounted against responses mounted against Ag in lymph blood-borne Ag filter Ag in lymph filter Ag in blood specialized for local can respond to systemic infections infectionsAg supplied by lymphatic Ag supplied by splenic artery system
  • 10. Lymphopoiesis (lymphocyte development): aprocess occurring within primary lymphoid organsin which lymphocytes differentiate from lymphoidstem cells, proliferate, and mature into functionalcells.
  • 11. fetalliver
  • 12. Systemic infection: a generic term for infectioncaused by microorganisms in animals or plants,where the causal agent (the microbe) has spreadactively or passively in the hosts anatomy, andis disseminated throughout several organs indifferent systems of the host.