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Ryan Spitler email@example.com 831-234-1496EDUCATION2010-Present University of California Irvine Irvine, CA Ph.D. student, Cellular and Molecular Biosciences2007 University of California Santa Cruz Santa Cruz, CA Bachelor of Science, Molecular Cell and Developmental Biology2005 Cabrillo College Aptos, CA Associate of Arts, ChemistryRESEARCH EXPERIENCE2010-Present University of California Irvine Irvine, CA Ph.D. Student • Development of a high-throughput automated imaging system to characterize therapeutics for the purpose of enhancing wound healing and to mitigate toxicity from smoke induced inhalation damage to the human lung epithelium. • Development of novel representative cellular and tissue models to study smoke induced inhalation damage.2008-2010 Stanford University Stanford, CA Life Science Research Assistant II • In vivo molecular imaging of small animals using the following devices: IVIS, MRI, CRI Maestro, Dual Axis Confocal (DAC) fluorescent microscope, Lucid Confocal, luminometer and ex vivo imaging using a bioluminescent microscope. • Use of an Nd:YAG laser for photocoagulation of mouse retina. • Use of flow cytometry for FAQS analysis. • Care and maintenance of laboratory mouse colonies. • Manage APLAC (Administrative Panel on Laboratory Animals Care), IRB (Institutional Review Board for human subject research compliance) and APB (Administrative Panel on Biosafety) protocols. • Manage one laboratory assistant for maintenance of lab stocks and miscellaneous lab upkeep. • Perform several techniques on mice such as: intradermal, subcutaneous, intraperitoneal, intravenous, retro-orbital injections, genotyping/screening, skin grafts and surgical removal of organs.
• Use/engineer fluorescent and bioluminescent mouse reporter models to answer specific biological questions. • Conduct standard laboratory techniques such as: plasmid preps, DNA/RNA isolations, and preparation of reagents. • Hold close collaborations with several local companies including: TransDerm Inc., SomaGenics Inc., and The Molecular Medicine Research Institute. • Completed several Stanford training courses and certifications related to animal and human research subjects. • Supervise and train new staff or students. This includes oversight and instruction on techniques as well as consultation on project work; serve as technical resource for other research staff; general lab maintenance: maintain lab stock, manage chemical inventory, safety records and provide general lab support as needed; may assist with purchasing laboratory equipment • I had the privilege of mentoring multiple summer students.2008-2009 TransDerm Inc. Santa Cruz, CA Research Assistant • Development of siRNA therapeutics for the treatment of skin diseases • Application methods used: high-pressure intradermal injection, microneedle array injection, iontophoresis, sonoporation and topical treatment. • Use of mouse, pig, and human tissues to examine various application technologies. • Evaluate functional efficacy of siRNA technologies primarily by RTqPCR of target genes and quantification of a reduction in bioluminescence or fluorescence. • Immuno-staining and fluorescent microscopy of sectioned tissue used to observe penetration of siRNA and siRNA mimics. • Growth and maintenance of primary keratinocytes for a human skin graft mouse model. • Assist with the writing of grants and publications.2005-2006 University of California Santa Cruz Santa Cruz, CA Lab Assistant • Conducted transformations using E.coli in order to obtain specific spliceosome associated proteins. • Screened Spliceosome associated proteins for solubility via western blot. • Purified soluble proteins via HPLC for x-ray crystallographic analysis.
INDUSTRIAL EXPERIENCE2008 TestAmerica Morgan Hill, CA Analyst I MicrobiologyConducted the following analysis techniques on water samples: • MPN determination by multiple tube fermentation and chromogenic substrate method in respect to Coliform. • Heterotrophic Plate Count • Presence/Absence for Coliform and E.coli. • Free and total Residual Chlorine • Dissolved Oxygen • Biological Oxygen Demand • Chemical Oxygen Demand • Chlorophyll • pH • Conductivity2007-2008 Santa Cruz Biotechnology Inc. Santa Cruz, CA Research Assistant • Aid in training of new employees. • Propagation and maintenance of cancer cell lines as well as hybridomas. • Prepare specialized media and culture cells following protocols designed to fulfill special requests from customers or in-house users. • Keep records of duties performed in laboratory notebooks and related computer databases. • Performed routine laboratory material and facility maintenance. • Used a flow cytometer for the determination of appropriate cell density for vialing of cell lines. • Transient transfections and inductions performed on cell lines.2003-2007 Santa Cruz Biotechnology Inc. Santa Cruz, CA Sr. Laboratory Assistant • Supervised part time task distribution and accurate completion as well as trained all new part time employees and several Research Assistants. • Lead countless special projects to improve quality and productivity of the lab as a whole.
• Composed and refined experimental protocols. • Most senior member of the lab group, primary troubleshooter involving lab techniques and results. • Isolated various monoclonal antibodies via affinity binding methods. • Inoculated mice with specific antigens and conducted preliminary screens for immune response via tail bleeds and ELISA. • Characterized monoclonal antibodies via western blot and antibody isotyping. • Expressed recombinant proteins using E. coli for antibody characterization via western blot.PUBLICATIONSAbstracts2010 Gordon Conference, Drug Carriers in Medicine & Biology Waterville Valley, NH (poster presentation) Development of RNAi Therapeutics for the Treatment of Liver Disease2010 Caliper Owners Meeting Group San Francisco, CA (Invited Speaker) The Development of RNAi Delivery Tools for Skin Diseases2010 Center for Biomedica lmaging at Stanford Symposium Stanford, CA (Invited Speaker) Development of RNAi Therapeutics for the Treatment of Liver Diseases2010 Pediatric Research Retreat Lucile Packard Children’s Hospital Stanford, CA (poster presentation) Development of RNAi Therapeutics for the Treatment of Liver Disease2009 BioX Interdisciplinary Poster Symposium Stanford, CA (poster presentation) Methods of RNAi Delivery for the Treatment of Liver DiseasesJOURNAL ARTICLES 1. Gunilla B Jacobson, Emilio Gonzalez-Gonzalez, Ryan Spitler, Rajesh Shinde, Devin Leake, Roger L Kaspar, Christopher H Contag, Richard N Zare. Biodegradable Nanoparticles with Sustained Release of Functional siRNA in Skin. Journal of Pharmeceutical Science 2010 Oct (10):4261-6. 2. Emilio González-González, Hyejun Ra, Ryan Spitler, Robyn P. Hickerson, Christopher H. Contag, and Roger L. Kaspar. Increased interstitial pressure improves nucleic acid delivery to skin enabling a comparative analysis of constitutive promoters. Gene Therapy 2010 Oct (10):1270-8.
3. Emilio Gonzalez-Gonzalez, Tycho J. Speaker, Robyn P. Hickerson, Ryan Spitler, Manny Flores, Devin Leake, Christopher H. Contag, and Roger L. Kaspar. Silencing of reporter gene expression in skin using siRNAs delivered by a hollow soluble protrusion array device (PAD). Journal of Molecular Therapy 2010 Sept 1667-1674. (work featured on the cover of the Sept edition) 4. (Mark Mackanos*, Christopher Sramek*), Ryan Spitler, Loh-Shan Leung, Hiroyuki Nomoto, Christopher H. Contag, Daniel Palanker. Non-damaging retinal phototherapy: dynamic range of the heat shock protein expression. (*authors contributed equally) Investigative Ophthalmology & Visual Science. 2010 Nov 18. 5. Ryan Spitler, Gunilla Jacobson, Heini Iives, Brian Johnston, Christopher H Contag. Nanoparticle Delivery of functional shRNA for the Treatment of HCV. (in preparation) 6. Emilio González-González, Yeu Chun Kim, Tycho J Speaker, Robyn P. Hickerson, Ryan Spitler, James Birchall, Maria Fernanda Lara, Mark R. Prausnitz, Leonard Milstone, Christopher H. Contag, and Roger L. Kaspar. New tools for testing delivery technologies of nucleic acids to skin: soluble versus steel microneedle arrays. (in preparation) 7. Sudipta Majumdar, Agnes Hajduczki, Rosemarie Vithayathil, Tivoli J. Olsen, Ryan M. Spitler, Aaron S. Mendez, Travis D. Thompson, Gregory A. Weiss. In vitro Evolution of Ligands to the Membrane Protein Caveolin. (Submitted Journal of the American Chemical Society, March 2011).EVENTS ATTENDED2010 Stanford Go Delivery Skin Symposium Stanford, CA2009 Institute for Stem Cell Biology and Regenerative Medicine Retreat Stanford, CA2009 MIPS (Molecular Imaging Program at Stanford) Retreat Asilomar, CA2009 Pachyonychia Congenita Patient Support Meeting Salt Lake City, UtahASSOCIATIONS2010-Present Beckman Laser Institute & Medical Clinic2008-2010 Member of the Pachyonychia Congenita Foundation2008-2010 Network for Translational Research in Optical Imaging2008-2010 Molecular Imaging Program at Stanford University
AWARDS2010 Center for Biomedical Imaging at Stanford University Achievement Award