Connective Tissue        DR. KIRTI SOLANKE
Connective Tissue•   Found everywhere•   Most abundant•   Development•   Functions    – Protection    – Support    – Bind ...
COMPONENTS                    LIVING                                        NON - LIVING                    CELLS         ...
Fibers• Collagen/ white fibers• Elastic/yellow fibers• Reticular fibers/Argyrophilic – fine collagen  fibers
COLLAGEN FIBRES• In bundles branch,1-12um in dia, White• H &E and Van Gieson:pink;masson’s  T:blue;• Tensile force,birefri...
SYNTHESIS• AA taken up by cells& linked PROα  CHAINS αchain 3chains join to form  PROCOLLAGEN MOL such mol leave  cell thr...
TYPESTYPE                LOCATIONI(250nm dia)        SKIN,BONE,TENDON,FASCIA,                    CAPSULEII(20 to 100nm)   ...
RETICULAR       FIBRES(Argyrophilic)• Collagen type III,Striation(68ηm),20ηm  diameter,do not bundle,uneven in  thickness....
RETICULAR FIBRES
ELASTIC FIBRES• Run singly,branches,0.1-0.2μm in dia• Not well stained H&E;Certain fixative  make them refractile then can...
WEIGERT’S STAIN
GROUND SUBSTANCE• Glycoprotiens:keratan s• Multiadhesive  glycoproteins:laminin,fibronectin• Proteoglycans:aggrecan,decori...
Ground Substance•   GSG linked with pr.•   Carry sulphate gr(so3-)Carboxyl gr(coo-).•   Thus proteoglycans r in long chain...
GLYCOSAMINOGLYCANSTISSUE   CHOND    DERMAT   HEPARA HEPARI    KERATA   HYALUR         ROITIN   AN       N      N         N...
HYALURONIC ACID• Hyaluronan:free carbohydrate chain• Polymers r very large• Synthesized by enzymes &not  posttranslatioall...
CELLS• FIXED TYPE:Fibroblast,Persistant  mesenchymal cells,Adipocytes.• WANDERING  CELLS:Lymphocyte,Monocyte,Mast  cell,Ma...
FIBROBLAST ADIPOCYTE
MACROPHAGE
MAST CELL
LYMPHOCYTE
PLASMA CELL
CLASSFICATION OF C. T.• Types of cells• Types of fibres• Amount of ground subs
CLASSIFICATION                      CONNECTIVE TISSUE                             ADULT C. T.    FETAL C. T.              ...
FETAL C.T:WARTON’JELLY
LOOSE AREOLAR T.
Loose Connective• Areolar Tissue  – Gel like matrix  – Fibroblasts, mast cells  – Collagen, elastic and reticular fibers  ...
Dense Connective• Dense Regular  – Parallel collagen fibers    with a few fibroblasts    and a few elastin fibers  – Attac...
DENCE REGULAR C.T.
TENDON
Dense Connective• Dense irregular  – Collagen fibers with a few elastic fibers    haphazardly arranged  – Strong in many d...
DENCE IRREGULAR C.T.
ADIPOSE TISSUE
APPLIED• SCURVY:Vit C DEFICIENCY• OSEOGENESIS IMPERFECTA:Brittle  bone disease,blue sclera hearing  loss,TYPE I asso• EHLE...
8 connective tissue
8 connective tissue
8 connective tissue
8 connective tissue
8 connective tissue
8 connective tissue
8 connective tissue
8 connective tissue
8 connective tissue
8 connective tissue
8 connective tissue
Upcoming SlideShare
Loading in …5
×

8 connective tissue

3,804 views

Published on

0 Comments
1 Like
Statistics
Notes
  • Be the first to comment

No Downloads
Views
Total views
3,804
On SlideShare
0
From Embeds
0
Number of Embeds
5
Actions
Shares
0
Downloads
26
Comments
0
Likes
1
Embeds 0
No embeds

No notes for slide

8 connective tissue

  1. 1. Connective Tissue DR. KIRTI SOLANKE
  2. 2. Connective Tissue• Found everywhere• Most abundant• Development• Functions – Protection – Support – Bind other tissues – Energy storage/insulation – Hormone production
  3. 3. COMPONENTS LIVING NON - LIVING CELLS MATRIX FIXED WANDERING FIBRES GRD SUBS FIBROBLASTS MACROPHAGES COLLAGEN MPS GLYCO PR FAT CELLS MAST CELLS ELASTIN SO4TANT MESENCHMAL CELLS PLASMA CELLS RETICULAR NON-SO4 PIGMENT CELLS NEUTROPHILS EOSINOPHILS
  4. 4. Fibers• Collagen/ white fibers• Elastic/yellow fibers• Reticular fibers/Argyrophilic – fine collagen fibers
  5. 5. COLLAGEN FIBRES• In bundles branch,1-12um in dia, White• H &E and Van Gieson:pink;masson’s T:blue;• Tensile force,birefringence,swell with weak alkali,boiling convert it into gelatin.• Synthesis:fibroblast,regulation,degradation (MMP)• Made of tropocollagen mol;made of 3 polypeptide chains(procollagen)
  6. 6. SYNTHESIS• AA taken up by cells& linked PROα CHAINS αchain 3chains join to form PROCOLLAGEN MOL such mol leave cell through secretory vacuoles to form TROPOCOLLAGEN MOL aggregate to form COLLAGEN FIBRILS.(vit C,oxy)• Fibrillogenesis
  7. 7. TYPESTYPE LOCATIONI(250nm dia) SKIN,BONE,TENDON,FASCIA, CAPSULEII(20 to 100nm) HYALINE CARTILAGE ,NOTOCHORD,INTERVERTEBRAL DISCIII RETICULAR FIBRES,FETAL SKIN,BLOOD VESSEL.IV BASAL LAMINA,KIDNEY GLOMERULI
  8. 8. RETICULAR FIBRES(Argyrophilic)• Collagen type III,Striation(68ηm),20ηm diameter,do not bundle,uneven in thickness.• Form network by branching• Silver impregnation:black but type I:brown• H&E:not identified;• More carbohydrates:PAS• Early mechanicalstrenth,delicate,suporting stroma in lymphatic T.(not thymus)• Synthesis:reticular cells
  9. 9. RETICULAR FIBRES
  10. 10. ELASTIC FIBRES• Run singly,branches,0.1-0.2μm in dia• Not well stained H&E;Certain fixative make them refractile then can be visualised• Composed of:central core of elastin & surrounding network of fibrillin microfibril• Lacks hydroxylysine,random distribution of glysine:HYDROPHOBIC & random coiling.• Vertebral ligaments,larynx,elastic A
  11. 11. WEIGERT’S STAIN
  12. 12. GROUND SUBSTANCE• Glycoprotiens:keratan s• Multiadhesive glycoproteins:laminin,fibronectin• Proteoglycans:aggrecan,decorinPr + long chain polysaccharide – glycosoaminoglycans (MPS) » Sulphated » Non sulphated
  13. 13. Ground Substance• GSG linked with pr.• Carry sulphate gr(so3-)Carboxyl gr(coo-).• Thus proteoglycans r in long chain,• Can retain water thus proteoglycans form• semi-solid, gel:stiffness• Molecular arrangement: sieve• Barrier:kidney;gas exchange:lungs
  14. 14. GLYCOSAMINOGLYCANSTISSUE CHOND DERMAT HEPARA HEPARI KERATA HYALUR ROITIN AN N N N S. NIC SULPHA SULPHA SULPHA ACID TE TE TETYPICAL + +CTCARTILA + + +GEBONE +SKIN + + + +BASEME +NT MOTHERS B/V LUNGS MAST C CORNEA SYNOVI INTER V AL DISC FLUID
  15. 15. HYALURONIC ACID• Hyaluronan:free carbohydrate chain• Polymers r very large• Synthesized by enzymes &not posttranslatioally modified• No sulfate,proteoglycan aggregates• So cartilage resist compression without inhibiting flexibility
  16. 16. CELLS• FIXED TYPE:Fibroblast,Persistant mesenchymal cells,Adipocytes.• WANDERING CELLS:Lymphocyte,Monocyte,Mast cell,Macrophages,Neutrphil,Plasma cell,Eosinophils.
  17. 17. FIBROBLAST ADIPOCYTE
  18. 18. MACROPHAGE
  19. 19. MAST CELL
  20. 20. LYMPHOCYTE
  21. 21. PLASMA CELL
  22. 22. CLASSFICATION OF C. T.• Types of cells• Types of fibres• Amount of ground subs
  23. 23. CLASSIFICATION CONNECTIVE TISSUE ADULT C. T. FETAL C. T. ORDINARY SPECIALISEDLOOSE C. T. DENSE C.T. BLOOD AREOLAR WHITE YELLOW CARTILAGE ADIPOSE REGULAR IRREGULAR BONE RETICULAR TENDON S/C TISSUE LIGAMENT APONEUROSIS
  24. 24. FETAL C.T:WARTON’JELLY
  25. 25. LOOSE AREOLAR T.
  26. 26. Loose Connective• Areolar Tissue – Gel like matrix – Fibroblasts, mast cells – Collagen, elastic and reticular fibers – Functions to wrap and cushion organs – Found in the lamina propria, around organs, capillaries
  27. 27. Dense Connective• Dense Regular – Parallel collagen fibers with a few fibroblasts and a few elastin fibers – Attach muscles to bones – Great tensile strength in one direction – Tendons and ligaments
  28. 28. DENCE REGULAR C.T.
  29. 29. TENDON
  30. 30. Dense Connective• Dense irregular – Collagen fibers with a few elastic fibers haphazardly arranged – Strong in many directions – Dermis, joint capsules, submucosa of digestive tract
  31. 31. DENCE IRREGULAR C.T.
  32. 32. ADIPOSE TISSUE
  33. 33. APPLIED• SCURVY:Vit C DEFICIENCY• OSEOGENESIS IMPERFECTA:Brittle bone disease,blue sclera hearing loss,TYPE I asso• EHLERS-DANLOS :hypermobility of joints of digit,TYPE III asso• MARFAN’S S:FBN1,fibrillin gene

×