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8 connective tissue

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  • 1. Connective Tissue DR. KIRTI SOLANKE
  • 2. Connective Tissue• Found everywhere• Most abundant• Development• Functions – Protection – Support – Bind other tissues – Energy storage/insulation – Hormone production
  • 3. COMPONENTS LIVING NON - LIVING CELLS MATRIX FIXED WANDERING FIBRES GRD SUBS FIBROBLASTS MACROPHAGES COLLAGEN MPS GLYCO PR FAT CELLS MAST CELLS ELASTIN SO4TANT MESENCHMAL CELLS PLASMA CELLS RETICULAR NON-SO4 PIGMENT CELLS NEUTROPHILS EOSINOPHILS
  • 4. Fibers• Collagen/ white fibers• Elastic/yellow fibers• Reticular fibers/Argyrophilic – fine collagen fibers
  • 5. COLLAGEN FIBRES• In bundles branch,1-12um in dia, White• H &E and Van Gieson:pink;masson’s T:blue;• Tensile force,birefringence,swell with weak alkali,boiling convert it into gelatin.• Synthesis:fibroblast,regulation,degradation (MMP)• Made of tropocollagen mol;made of 3 polypeptide chains(procollagen)
  • 6. SYNTHESIS• AA taken up by cells& linked PROα CHAINS αchain 3chains join to form PROCOLLAGEN MOL such mol leave cell through secretory vacuoles to form TROPOCOLLAGEN MOL aggregate to form COLLAGEN FIBRILS.(vit C,oxy)• Fibrillogenesis
  • 7. TYPESTYPE LOCATIONI(250nm dia) SKIN,BONE,TENDON,FASCIA, CAPSULEII(20 to 100nm) HYALINE CARTILAGE ,NOTOCHORD,INTERVERTEBRAL DISCIII RETICULAR FIBRES,FETAL SKIN,BLOOD VESSEL.IV BASAL LAMINA,KIDNEY GLOMERULI
  • 8. RETICULAR FIBRES(Argyrophilic)• Collagen type III,Striation(68ηm),20ηm diameter,do not bundle,uneven in thickness.• Form network by branching• Silver impregnation:black but type I:brown• H&E:not identified;• More carbohydrates:PAS• Early mechanicalstrenth,delicate,suporting stroma in lymphatic T.(not thymus)• Synthesis:reticular cells
  • 9. RETICULAR FIBRES
  • 10. ELASTIC FIBRES• Run singly,branches,0.1-0.2μm in dia• Not well stained H&E;Certain fixative make them refractile then can be visualised• Composed of:central core of elastin & surrounding network of fibrillin microfibril• Lacks hydroxylysine,random distribution of glysine:HYDROPHOBIC & random coiling.• Vertebral ligaments,larynx,elastic A
  • 11. WEIGERT’S STAIN
  • 12. GROUND SUBSTANCE• Glycoprotiens:keratan s• Multiadhesive glycoproteins:laminin,fibronectin• Proteoglycans:aggrecan,decorinPr + long chain polysaccharide – glycosoaminoglycans (MPS) » Sulphated » Non sulphated
  • 13. Ground Substance• GSG linked with pr.• Carry sulphate gr(so3-)Carboxyl gr(coo-).• Thus proteoglycans r in long chain,• Can retain water thus proteoglycans form• semi-solid, gel:stiffness• Molecular arrangement: sieve• Barrier:kidney;gas exchange:lungs
  • 14. GLYCOSAMINOGLYCANSTISSUE CHOND DERMAT HEPARA HEPARI KERATA HYALUR ROITIN AN N N N S. NIC SULPHA SULPHA SULPHA ACID TE TE TETYPICAL + +CTCARTILA + + +GEBONE +SKIN + + + +BASEME +NT MOTHERS B/V LUNGS MAST C CORNEA SYNOVI INTER V AL DISC FLUID
  • 15. HYALURONIC ACID• Hyaluronan:free carbohydrate chain• Polymers r very large• Synthesized by enzymes &not posttranslatioally modified• No sulfate,proteoglycan aggregates• So cartilage resist compression without inhibiting flexibility
  • 16. CELLS• FIXED TYPE:Fibroblast,Persistant mesenchymal cells,Adipocytes.• WANDERING CELLS:Lymphocyte,Monocyte,Mast cell,Macrophages,Neutrphil,Plasma cell,Eosinophils.
  • 17. FIBROBLAST ADIPOCYTE
  • 18. MACROPHAGE
  • 19. MAST CELL
  • 20. LYMPHOCYTE
  • 21. PLASMA CELL
  • 22. CLASSFICATION OF C. T.• Types of cells• Types of fibres• Amount of ground subs
  • 23. CLASSIFICATION CONNECTIVE TISSUE ADULT C. T. FETAL C. T. ORDINARY SPECIALISEDLOOSE C. T. DENSE C.T. BLOOD AREOLAR WHITE YELLOW CARTILAGE ADIPOSE REGULAR IRREGULAR BONE RETICULAR TENDON S/C TISSUE LIGAMENT APONEUROSIS
  • 24. FETAL C.T:WARTON’JELLY
  • 25. LOOSE AREOLAR T.
  • 26. Loose Connective• Areolar Tissue – Gel like matrix – Fibroblasts, mast cells – Collagen, elastic and reticular fibers – Functions to wrap and cushion organs – Found in the lamina propria, around organs, capillaries
  • 27. Dense Connective• Dense Regular – Parallel collagen fibers with a few fibroblasts and a few elastin fibers – Attach muscles to bones – Great tensile strength in one direction – Tendons and ligaments
  • 28. DENCE REGULAR C.T.
  • 29. TENDON
  • 30. Dense Connective• Dense irregular – Collagen fibers with a few elastic fibers haphazardly arranged – Strong in many directions – Dermis, joint capsules, submucosa of digestive tract
  • 31. DENCE IRREGULAR C.T.
  • 32. ADIPOSE TISSUE
  • 33. APPLIED• SCURVY:Vit C DEFICIENCY• OSEOGENESIS IMPERFECTA:Brittle bone disease,blue sclera hearing loss,TYPE I asso• EHLERS-DANLOS :hypermobility of joints of digit,TYPE III asso• MARFAN’S S:FBN1,fibrillin gene