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Final presentation Final presentation Presentation Transcript

  • Assessment of Spider Families Clubionidae, Lycosidae & Theridiidae as Potential Biocontrol Agents of Brassica Pests Madaline Healey
  • Project Aim
    • “ to determine the potential of Clubionidae, Lycosidae and Theridiidae to control
    • Plutella xylostella,
    • Crocidolomia pavonana &
    • Myzus persicae
    • in Brassica vegetable crops….”
  • Brassica Vegetable Pests
    • Diamondback moth
    • Plutella xylostella
    • Cabbage Cluster Caterpillar
    • Crocidolomia pavonana
    • Green Peach Aphid
    • Myzus persicae
  • Brassica Vegetable Pests
    • Diamondback Moth
    • P. xylostella
    • Cabbage Cluster Caterpillar
    • C. pavonana
    • Green Peach Aphid
    • M. persicae
    Diamondback larvae damage
  • Brassica Vegetable Pests
    • Diamondback Moth
    • P. xylostella
    • Cabbage Cluster Caterpillar
    • C. pavonana
    • Green Peach Aphid
    • M. persicae
    Cabbage cluster larvae damage
  • Brassica Vegetable Pests
    • Diamondback moth
    • Plutella xylostella
    • Cabbage cluster caterpillar
    • Crocidolomia pavonana
    • Green Peach Aphid
    • M. persicae
    Green peach nymph damage
  • Background
    • Chemical resistance
    • South East QLD Lockyer Valley survey
      • 70% utilise IPM
      • 30% consciously protect natural enemies
    • Spider dominant natural enemy in Brassica vegetables
  • Spiders in Brassica Vegetables
      • Clubionidae
      • -hunting plant dweller
      • Lycosidae
      • -hunting ground dweller
      • Theridiidae
      • -tangle web-weaver
  • Spiders in Brassica Vegetables
      • Clubionidae
      • - hunting plant dweller
      • Lycosidae
      • -hunting ground dweller
      • Theridiidae
      • -tangle web-weaver
  • Spiders in Brassica Vegetables
      • Clubionidae
      • -hunting plant dweller
      • Lycosidae
      • - hunting ground dweller
      • Theridiidae
      • -tangle web-weaver
  • Spiders in Brassica Vegetables
      • Clubionidae
      • -hunting plant dweller
      • Lycosidae
      • -hunting ground dweller
      • Theridiidae
      • -tangle web-weaver
  • Spiders in Brassica Vegetables
      • What impact will these spiders have in reducing pests?
  • Objectives
    • determine the ability of three spider families to reduce pest populations
    • determine if a connection exists between spider and prey preference
    • determine spider predation potential as a naturally occurring biocontrol agent in Brassica vegetables
  • Laboratory Experiments
    • Exp 1
    • No choice Lepidoptera predation
    • Diamondback & Cabbage cluster
    • Five larvae per trt
    • Six trts, 2 controls
    • Consumption after 24 hours
    • Exp 2
    • Lepidoptera prey preference
    • Diamondback & Cabbage cluster
    • One larva of each prey
    • Three trts, one control
    • First prey attacked
    • Exp 3
    • Lepidoptera prey preference
    • by Lycosidae
    • Diamondback & Cabbage cluster
    • One larva each prey
    • One trt, one control
    • First prey attacked
    • Exp 4
    • Prey species preference
    • Cabbage cluster & Aphid
    • One larva each prey
    • Three trts, one control
    • First prey attacked
  • Experimental Collection
    • Colonies
      • Diamondback moth
      • Green peach aphid
    • Organic broccoli plantings
      • Spiders
      • Cabbage cluster egg rafts
    Cabbage cluster egg raft
  • Experimental Arena Caged arena with broccoli seedling
  • Experiment One Lepidoptera predation
    • Two-factor ANOVA
    • No significant difference between prey mortality
    • P = 0.715
    • No interaction
    • P = 0.195
    • Spider families will
    • readily predate both
    • prey
    • No significant difference between treatments
      • LSD = 1.361
  • Experiment Two Lepidoptera prey preference
    • Chi-square analysis
    • Significant prey preference
      • Clear choice of prey dependant on spider type
      • χ ² = 0.010
    • DBM – Diamondback moth
    • CCC – Cabbage cluster
  • Experiment Three Lycosidae prey preference
    • Chi-square analysis
    • No significant prey preference
      • Prey choice independent of spider type
      • χ ² = 0.157
  • Experiment Four Prey species preference
    • Chi-square analysis
    • No significant prey preference
      • Prey choice independent of spider type
      • χ ² = 0.117
    • CCC – Cabbage cluster
    • GPA – Green peach aphid
  • General Discussion
    • All spider treatments will consume three Brassica pests
      • Generalist predator more effective than selective
    • Prey preference
      • Physical and behavioural characteristics
        • Diamondback larvae active
        • Cabbage cluster larvae sluggish
        • Green peach aphid nymphs passive
  • Future Research
    • Repeat experiments
      • increase experimental
    • units
    • Lycosidae on plant
  • Future Research
    • Cage exclusion
    • trials
    • Spider thresholds
      • No. spider per metre ²
    Semi-excluded cage
  • Summary
    • Clubionidae, Lycosidae & Theridiidae will predate
    • Plutella xylostella
    • Crocidolomia pavonana
    • Myzus persicae
    • Potential naturally occurring biological control agent
  • Acknowledgements
    • Dr. Graham Brodie
    • University of Melbourne
    • Dr. Lara Senior
    • Agri-Science Queensland
    • Ms. M. Firell
    • Agri-Science Queensland