“ Conventional” sitting drop method was introduced by Bob Bock in 1968 (former Dean of the Graduate School at UW)
Hanging drop method was developed in 1971
This became the standard method for crystallization by 1972
Hanging Drop Method of Vapor Diffusion This is the most commonly used technique for surveying and growing crystals for structural analyses.
X-ray Diffraction is the Best Test for Crystallinity
Most protein crystals are grown with the goal of determining their structure, thus an x-ray diffraction experiment is considered to be the ultimate proof that a well ordered crystalline lattice has been achieved.