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OPC Maturation Article Presentation
OPC Maturation Article Presentation
OPC Maturation Article Presentation
OPC Maturation Article Presentation
OPC Maturation Article Presentation
OPC Maturation Article Presentation
OPC Maturation Article Presentation
OPC Maturation Article Presentation
OPC Maturation Article Presentation
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OPC Maturation Article Presentation

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Presentation for the Nature paper: Bergles, et al. Glutamatergic synapses on …

Presentation for the Nature paper: Bergles, et al. Glutamatergic synapses on
oligodendrocyte precursor
cells in the hippocampus, request download for animations

Published in: Education, Business
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  • 1. <ul><li>Glutamatergic synapses on </li></ul><ul><li>oligodendrocyte precursor </li></ul><ul><li>cells in the hippocampus </li></ul><ul><li>Dwight E. Bergles*, J. David B. Roberts², Peter Somogyi²&amp; Craig E. Jahr* </li></ul><ul><li>* Vollum Institute, L474, Oregon Health Sciences University, Portland, </li></ul><ul><li>Oregon 97201, USA </li></ul><ul><li>²MRC Anatomical Neuropharmacology Unit, Department of Pharmacology, </li></ul><ul><li>University of Oxford, Oxford OX1 3TH, UK </li></ul><ul><li>NATURE |VOL 405 | 11 MAY 2000 |www.nature.com </li></ul>HHMI-IMBS Presentation: Richard Myers
  • 2. In vitro OPC maturation is blocked by glutamate Ca2+ ion AMPA Receptor Ca2+ Channel Glutamate Molecule Ca2+ ion X Glutamate Molecule OPC Mature Oligodendrocyte Inward Current X
  • 3. Currents in NG2+ OPCs Figure 1 Synaptic responses from identi®ed OPCs in hippocampal slices. a, Currentclamp recording of membrane responses to current injection (-80 to 140 pA; step size, 20 pA). b, Evoked responses to Schaffer collateral/commissural ®bre stimulation, recorded in voltage-clamp (holding potential, -90 mV; lower trace) and current-clamp (membrane potential = -90 mV; upper trace). Traces are averages of 15 consecutive responses recorded from the same cell. Stimulus: 30mA, 100ms. c, Reconstruction of a biocytin-®lled OPC. d, Micrograph of the same OPC as in d, visualized by AMCAconjugated streptavidin. e, NG2-immunoreactivity of same region of the slice as shown in d. Scale bars for c and d, 20mm; d and e are at the same magni®cation.
  • 4. Neuron to OPC signal in rat brain <ul><li>CA3 neurons were stimulated with Glutamate </li></ul><ul><li>Signal was relayed to CA1 neurons and then OPCs </li></ul><ul><li>OPC membrane current was measured </li></ul><ul><li>Currents abolished by TTX (Na+ channel blocker) </li></ul>OPC
  • 5. AMPA receptors on OPCs receive glutamate signals AMPA Receptor Ca2+ Channel Ca2+ ion Glutamate Molecule Ca2+ ion X OPC Inward Current Blocked X NBQX (AMPA Receptor Inhibitor) X
  • 6. Glutamate induced currents in OPCs requires Ca2+ influx Cd2+ ion AMPA Receptor Ca2+ Channel Ca2+ ion Glutamate Molecule Ca2+ ion X OPC Inward Current Blocked X Glutamate Molecule Cd2+ ion
  • 7. EM of Neuron-OPC synaptic interation <ul><li>b : Axon bouton forming a synpase </li></ul><ul><li>s : dendritic spine from neighboring neuron </li></ul><ul><li>black: biocytin labeled OPC </li></ul><ul><li>Notice the glutamate vesicles forming at the bouton membrane </li></ul>
  • 8. EM of Neuron-OPC synaptic interation <ul><li>OPC labeled with NG2 silver stain </li></ul><ul><li>f : OPC targeted by vesicle signaling as opposed to neighboring neuron </li></ul><ul><li>OPC membrane is thinner than neighboring neurons </li></ul>
  • 9. Neurons control self-myelination through regulation of OPC maturation Mature Oligodendrocyte OPCs do not mature OPC Maturation Demyelination Remyelination X

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