Key to engineering vasc tree is bioprinting - building blocks, must be living tissue and without “mortar” to hold them in place
(bio in a broad sense meaning synthetic biocompatible materials, natural extra-cellular matrices, cells, genes and proteins/peptides)
Also experience in developmental biology to know how to direct building blocks into specific tissue types - fuse seamlessly- began with laser jet printers in which print cartidges were filled with cells.
Our bioink comes in different colors representing diff. stem populations - building blocks are spheroids of 100K cells (breakthough) – can be dispensed by robotic printers – unlike brick which requires mortar, we were able to show several years ago that living building blocks or spheres will fuse seamlessly within a layer and between layers - come in different colors so to speak in printing an organ you need function tissue that carries out fc like absorption, excretion, contraction (point our gold blocks) – but the functional tissue needs oxygen which means blood supply – our plan thus is to first engineer the green tissue, the vasculare tissue , then fill in with functional gold blocks - thus the SC project is to print engineer the vascu tissue
vHow it will be pursued,, envision biofabrication or bp as requiring 3 major steps pre proc post called them thrusts note that computer cyberstructure important in first step, must start with blueprint that is computer designed / bioink is stem cells biopaper is hydrogel or biomaterials added to aggregrates that contain cues for what to become, lineage signals, biopaper could also be scaffold needed for larger bore vessels -- most challenging steps will be postprocessing – bioreactor will be key for maturation as it will allow for shear stress, flow/hemodynamics to act on printed structure, can accelerate maturation in a bioreactor but to accomplish rapid prototyping, have to find a way to reduce what takes nature weeks or more to just days – for this much of the scientific thrust of the project will be to isolate and introduce maturogens – what are these?? Molecules nature delivers slowly over time to promote maturation, we have isolated some of them and will deliver in larger amounts, more quickly
ATBC IS THE STRATEGIC HUB FOR IMPLEMENTING THE STRATEGIC THEME ENTER ---- based at MUSC ATBC WILL BE A PHYSICAL PLACE WHERE TECHNOLOGIES NEEDED TO ENGINEER VASCULAR TREE CAN BE PERFORMED OR COORDINATED NEEDED TO PERFORM THRUSTS WILL HOUSED IN ATBC /// KEY NEW BIOE BLDG = RECRUIT NEW TALENT //ANOTHER KEY WILL BE SUSTAINABILITY – REASONS NSF MADE SUCH A LARGE INVESTMENT IS THAT IT HAS REAL POTENTIAL TO EXPAND WORKFORCE BROADEN PARTICIPATION TO INCLUDE EVERYONE ALL THIS AND MORE BUT ALSO BEC IT IS AN ETHICHAL AND SCIENTIFICALLY SOUND SOLUTION TO SOLVING WHAT HAPPENS WHEN ORGANS FAIL AND THERE IS ONLY LIMITED SUPPLY OF DONOR TISSUES FOR TRANSPLANT NOT ONLY WILL SCIENCE BE COORDINATED BY ATBC BUT ALSO OUTREACH ACTIVITIES INCLUDING EDUCATION, DIVERSITY AND INDUSTRIAL THUS ATBC IS THE “NODAL” PLAYER IN STRATEGIC PLAN NEXT SLIDE SHOWS THE FLOOR PLAN FOR ATBC
GEAR GRANTS & RELATIONSHIP TO CURRENT NSF RII GRANT
GEAR GRANTS <ul><li>Due March 29 2010 </li></ul><ul><li>Pilot projects targeted to submitting an extramural grant within 12 months </li></ul><ul><li>Amount of awards (50-100K) </li></ul><ul><li>Peer Reviewed </li></ul><ul><li>10 pages max (interdisciplinary) </li></ul><ul><li>ALIGN with NSF RII Track 1 Award </li></ul>
GEAR GRANTS <ul><li>Instructions (SC EPSCOR OFFICE) </li></ul><ul><li>Scott Little ( [email_address] ) </li></ul><ul><li>Isabel Sanchez ( [email_address] ) </li></ul><ul><li>Key : alignment with the NSF RII grant “The South Carolina Project” </li></ul>
NSF-RII Grant Title “ South Carolina Project” Theme Tissue Based Engineering Goal To Engineer a Vascular Tree by Bioprinting
What is Bioprinting? Bioprinting/Biofabrication is a computer-aided, robotic layer by layer addition of living building blocks to form 3D functional human tissue constructs
BIOPRINTING the ability to arrange bio-materials in a controlled but “additive” fashion in three-dimensional (3D) space.
BioPrinting Using “BioInk” BioInk = aggregates of stem cells + hydrogel (morphogens)
A B C D E F G From Mironov, Markwald et al 2009 in Expert Opinion in Biological Therapy (in press) BioInk BioInk Stem cells + hydrogel
Digital Anatomy Blueprint CAD Bioink Bioprinter Biopaper MATUROGENS Tissue Constructs Biomonitoring I. Pre-processing II. Processing III. Post-processing Components of Organ Printing Technology Bioreactors
Enabling Technologies Required for Engineering a Vascular Tree Vascular Tree Bio Ink Bio Paper Bio Reactor Bio Monitor Bio informatics