Presentation summer 2011

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  • Within those we found coagulation disorders like AT responsable for heart attacks and strokes wich...\nAlso VT . In Dr. Weyrich’s Lab we investigates the coagulation process and its associated inflammation responses in order to unsdestand such diseases.\n
  • For those unfamiliar with the coagulation process i will try to explained in a simple way. First you have your blood in vessels and all its components like RBC, WBC, Platelets, Coagulations Factors: importants ones: Thombin and Fibrinogen. > When you get and injury in one of your vessels which happens many times each day, platelets recognizes some of the component in the subendothelial exposed layer and they aggregated forming a platelet plug, then they start secreting coagulation factors that leds to the activation of Thombin. Thrombin causes polymerization of fibrinogen molecules into fibrin fibers forming a meshwork that entraps blood cells, platelets and plasma, that is a clot, sealing your vessels. Monocytes interact with the clot, this led an inflammatory response that in our lab we are interested in. Led to Associated inflammatory response with the clot.\n
  • For those unfamiliar with the coagulation process i will try to explained in a simple way. First you have your blood in vessels and all its components like RBC, WBC, Platelets, Coagulations Factors: importants ones: Thombin and Fibrinogen. > When you get and injury in one of your vessels which happens many times each day, platelets recognizes some of the component in the subendothelial exposed layer and they aggregated forming a platelet plug, then they start secreting coagulation factors that leds to the activation of Thombin. Thrombin causes polymerization of fibrinogen molecules into fibrin fibers forming a meshwork that entraps blood cells, platelets and plasma, that is a clot, sealing your vessels. Monocytes interact with the clot, this led an inflammatory response that in our lab we are interested in. Led to Associated inflammatory response with the clot.\n
  • For those unfamiliar with the coagulation process i will try to explained in a simple way. First you have your blood in vessels and all its components like RBC, WBC, Platelets, Coagulations Factors: importants ones: Thombin and Fibrinogen. > When you get and injury in one of your vessels which happens many times each day, platelets recognizes some of the component in the subendothelial exposed layer and they aggregated forming a platelet plug, then they start secreting coagulation factors that leds to the activation of Thombin. Thrombin causes polymerization of fibrinogen molecules into fibrin fibers forming a meshwork that entraps blood cells, platelets and plasma, that is a clot, sealing your vessels. Monocytes interact with the clot, this led an inflammatory response that in our lab we are interested in. Led to Associated inflammatory response with the clot.\n
  • For those unfamiliar with the coagulation process i will try to explained in a simple way. First you have your blood in vessels and all its components like RBC, WBC, Platelets, Coagulations Factors: importants ones: Thombin and Fibrinogen. > When you get and injury in one of your vessels which happens many times each day, platelets recognizes some of the component in the subendothelial exposed layer and they aggregated forming a platelet plug, then they start secreting coagulation factors that leds to the activation of Thombin. Thrombin causes polymerization of fibrinogen molecules into fibrin fibers forming a meshwork that entraps blood cells, platelets and plasma, that is a clot, sealing your vessels. Monocytes interact with the clot, this led an inflammatory response that in our lab we are interested in. Led to Associated inflammatory response with the clot.\n
  • For those unfamiliar with the coagulation process i will try to explained in a simple way. First you have your blood in vessels and all its components like RBC, WBC, Platelets, Coagulations Factors: importants ones: Thombin and Fibrinogen. > When you get and injury in one of your vessels which happens many times each day, platelets recognizes some of the component in the subendothelial exposed layer and they aggregated forming a platelet plug, then they start secreting coagulation factors that leds to the activation of Thombin. Thrombin causes polymerization of fibrinogen molecules into fibrin fibers forming a meshwork that entraps blood cells, platelets and plasma, that is a clot, sealing your vessels. Monocytes interact with the clot, this led an inflammatory response that in our lab we are interested in. Led to Associated inflammatory response with the clot.\n
  • For those unfamiliar with the coagulation process i will try to explained in a simple way. First you have your blood in vessels and all its components like RBC, WBC, Platelets, Coagulations Factors: importants ones: Thombin and Fibrinogen. > When you get and injury in one of your vessels which happens many times each day, platelets recognizes some of the component in the subendothelial exposed layer and they aggregated forming a platelet plug, then they start secreting coagulation factors that leds to the activation of Thombin. Thrombin causes polymerization of fibrinogen molecules into fibrin fibers forming a meshwork that entraps blood cells, platelets and plasma, that is a clot, sealing your vessels. Monocytes interact with the clot, this led an inflammatory response that in our lab we are interested in. Led to Associated inflammatory response with the clot.\n
  • For those unfamiliar with the coagulation process i will try to explained in a simple way. First you have your blood in vessels and all its components like RBC, WBC, Platelets, Coagulations Factors: importants ones: Thombin and Fibrinogen. > When you get and injury in one of your vessels which happens many times each day, platelets recognizes some of the component in the subendothelial exposed layer and they aggregated forming a platelet plug, then they start secreting coagulation factors that leds to the activation of Thombin. Thrombin causes polymerization of fibrinogen molecules into fibrin fibers forming a meshwork that entraps blood cells, platelets and plasma, that is a clot, sealing your vessels. Monocytes interact with the clot, this led an inflammatory response that in our lab we are interested in. Led to Associated inflammatory response with the clot.\n
  • For those unfamiliar with the coagulation process i will try to explained in a simple way. First you have your blood in vessels and all its components like RBC, WBC, Platelets, Coagulations Factors: importants ones: Thombin and Fibrinogen. > When you get and injury in one of your vessels which happens many times each day, platelets recognizes some of the component in the subendothelial exposed layer and they aggregated forming a platelet plug, then they start secreting coagulation factors that leds to the activation of Thombin. Thrombin causes polymerization of fibrinogen molecules into fibrin fibers forming a meshwork that entraps blood cells, platelets and plasma, that is a clot, sealing your vessels. Monocytes interact with the clot, this led an inflammatory response that in our lab we are interested in. Led to Associated inflammatory response with the clot.\n
  • For those unfamiliar with the coagulation process i will try to explained in a simple way. First you have your blood in vessels and all its components like RBC, WBC, Platelets, Coagulations Factors: importants ones: Thombin and Fibrinogen. > When you get and injury in one of your vessels which happens many times each day, platelets recognizes some of the component in the subendothelial exposed layer and they aggregated forming a platelet plug, then they start secreting coagulation factors that leds to the activation of Thombin. Thrombin causes polymerization of fibrinogen molecules into fibrin fibers forming a meshwork that entraps blood cells, platelets and plasma, that is a clot, sealing your vessels. Monocytes interact with the clot, this led an inflammatory response that in our lab we are interested in. Led to Associated inflammatory response with the clot.\n
  • For those unfamiliar with the coagulation process i will try to explained in a simple way. First you have your blood in vessels and all its components like RBC, WBC, Platelets, Coagulations Factors: importants ones: Thombin and Fibrinogen. > When you get and injury in one of your vessels which happens many times each day, platelets recognizes some of the component in the subendothelial exposed layer and they aggregated forming a platelet plug, then they start secreting coagulation factors that leds to the activation of Thombin. Thrombin causes polymerization of fibrinogen molecules into fibrin fibers forming a meshwork that entraps blood cells, platelets and plasma, that is a clot, sealing your vessels. Monocytes interact with the clot, this led an inflammatory response that in our lab we are interested in. Led to Associated inflammatory response with the clot.\n
  • For those unfamiliar with the coagulation process i will try to explained in a simple way. First you have your blood in vessels and all its components like RBC, WBC, Platelets, Coagulations Factors: importants ones: Thombin and Fibrinogen. > When you get and injury in one of your vessels which happens many times each day, platelets recognizes some of the component in the subendothelial exposed layer and they aggregated forming a platelet plug, then they start secreting coagulation factors that leds to the activation of Thombin. Thrombin causes polymerization of fibrinogen molecules into fibrin fibers forming a meshwork that entraps blood cells, platelets and plasma, that is a clot, sealing your vessels. Monocytes interact with the clot, this led an inflammatory response that in our lab we are interested in. Led to Associated inflammatory response with the clot.\n
  • For those unfamiliar with the coagulation process i will try to explained in a simple way. First you have your blood in vessels and all its components like RBC, WBC, Platelets, Coagulations Factors: importants ones: Thombin and Fibrinogen. > When you get and injury in one of your vessels which happens many times each day, platelets recognizes some of the component in the subendothelial exposed layer and they aggregated forming a platelet plug, then they start secreting coagulation factors that leds to the activation of Thombin. Thrombin causes polymerization of fibrinogen molecules into fibrin fibers forming a meshwork that entraps blood cells, platelets and plasma, that is a clot, sealing your vessels. Monocytes interact with the clot, this led an inflammatory response that in our lab we are interested in. Led to Associated inflammatory response with the clot.\n
  • For those unfamiliar with the coagulation process i will try to explained in a simple way. First you have your blood in vessels and all its components like RBC, WBC, Platelets, Coagulations Factors: importants ones: Thombin and Fibrinogen. > When you get and injury in one of your vessels which happens many times each day, platelets recognizes some of the component in the subendothelial exposed layer and they aggregated forming a platelet plug, then they start secreting coagulation factors that leds to the activation of Thombin. Thrombin causes polymerization of fibrinogen molecules into fibrin fibers forming a meshwork that entraps blood cells, platelets and plasma, that is a clot, sealing your vessels. Monocytes interact with the clot, this led an inflammatory response that in our lab we are interested in. Led to Associated inflammatory response with the clot.\n
  • For those unfamiliar with the coagulation process i will try to explained in a simple way. First you have your blood in vessels and all its components like RBC, WBC, Platelets, Coagulations Factors: importants ones: Thombin and Fibrinogen. > When you get and injury in one of your vessels which happens many times each day, platelets recognizes some of the component in the subendothelial exposed layer and they aggregated forming a platelet plug, then they start secreting coagulation factors that leds to the activation of Thombin. Thrombin causes polymerization of fibrinogen molecules into fibrin fibers forming a meshwork that entraps blood cells, platelets and plasma, that is a clot, sealing your vessels. Monocytes interact with the clot, this led an inflammatory response that in our lab we are interested in. Led to Associated inflammatory response with the clot.\n
  • For those unfamiliar with the coagulation process i will try to explained in a simple way. First you have your blood in vessels and all its components like RBC, WBC, Platelets, Coagulations Factors: importants ones: Thombin and Fibrinogen. > When you get and injury in one of your vessels which happens many times each day, platelets recognizes some of the component in the subendothelial exposed layer and they aggregated forming a platelet plug, then they start secreting coagulation factors that leds to the activation of Thombin. Thrombin causes polymerization of fibrinogen molecules into fibrin fibers forming a meshwork that entraps blood cells, platelets and plasma, that is a clot, sealing your vessels. Monocytes interact with the clot, this led an inflammatory response that in our lab we are interested in. Led to Associated inflammatory response with the clot.\n
  • For those unfamiliar with the coagulation process i will try to explained in a simple way. First you have your blood in vessels and all its components like RBC, WBC, Platelets, Coagulations Factors: importants ones: Thombin and Fibrinogen. > When you get and injury in one of your vessels which happens many times each day, platelets recognizes some of the component in the subendothelial exposed layer and they aggregated forming a platelet plug, then they start secreting coagulation factors that leds to the activation of Thombin. Thrombin causes polymerization of fibrinogen molecules into fibrin fibers forming a meshwork that entraps blood cells, platelets and plasma, that is a clot, sealing your vessels. Monocytes interact with the clot, this led an inflammatory response that in our lab we are interested in. Led to Associated inflammatory response with the clot.\n
  • For those unfamiliar with the coagulation process i will try to explained in a simple way. First you have your blood in vessels and all its components like RBC, WBC, Platelets, Coagulations Factors: importants ones: Thombin and Fibrinogen. > When you get and injury in one of your vessels which happens many times each day, platelets recognizes some of the component in the subendothelial exposed layer and they aggregated forming a platelet plug, then they start secreting coagulation factors that leds to the activation of Thombin. Thrombin causes polymerization of fibrinogen molecules into fibrin fibers forming a meshwork that entraps blood cells, platelets and plasma, that is a clot, sealing your vessels. Monocytes interact with the clot, this led an inflammatory response that in our lab we are interested in. Led to Associated inflammatory response with the clot.\n
  • For those unfamiliar with the coagulation process i will try to explained in a simple way. First you have your blood in vessels and all its components like RBC, WBC, Platelets, Coagulations Factors: importants ones: Thombin and Fibrinogen. > When you get and injury in one of your vessels which happens many times each day, platelets recognizes some of the component in the subendothelial exposed layer and they aggregated forming a platelet plug, then they start secreting coagulation factors that leds to the activation of Thombin. Thrombin causes polymerization of fibrinogen molecules into fibrin fibers forming a meshwork that entraps blood cells, platelets and plasma, that is a clot, sealing your vessels. Monocytes interact with the clot, this led an inflammatory response that in our lab we are interested in. Led to Associated inflammatory response with the clot.\n
  • For those unfamiliar with the coagulation process i will try to explained in a simple way. First you have your blood in vessels and all its components like RBC, WBC, Platelets, Coagulations Factors: importants ones: Thombin and Fibrinogen. > When you get and injury in one of your vessels which happens many times each day, platelets recognizes some of the component in the subendothelial exposed layer and they aggregated forming a platelet plug, then they start secreting coagulation factors that leds to the activation of Thombin. Thrombin causes polymerization of fibrinogen molecules into fibrin fibers forming a meshwork that entraps blood cells, platelets and plasma, that is a clot, sealing your vessels. Monocytes interact with the clot, this led an inflammatory response that in our lab we are interested in. Led to Associated inflammatory response with the clot.\n
  • For those unfamiliar with the coagulation process i will try to explained in a simple way. First you have your blood in vessels and all its components like RBC, WBC, Platelets, Coagulations Factors: importants ones: Thombin and Fibrinogen. > When you get and injury in one of your vessels which happens many times each day, platelets recognizes some of the component in the subendothelial exposed layer and they aggregated forming a platelet plug, then they start secreting coagulation factors that leds to the activation of Thombin. Thrombin causes polymerization of fibrinogen molecules into fibrin fibers forming a meshwork that entraps blood cells, platelets and plasma, that is a clot, sealing your vessels. Monocytes interact with the clot, this led an inflammatory response that in our lab we are interested in. Led to Associated inflammatory response with the clot.\n
  • For those unfamiliar with the coagulation process i will try to explained in a simple way. First you have your blood in vessels and all its components like RBC, WBC, Platelets, Coagulations Factors: importants ones: Thombin and Fibrinogen. > When you get and injury in one of your vessels which happens many times each day, platelets recognizes some of the component in the subendothelial exposed layer and they aggregated forming a platelet plug, then they start secreting coagulation factors that leds to the activation of Thombin. Thrombin causes polymerization of fibrinogen molecules into fibrin fibers forming a meshwork that entraps blood cells, platelets and plasma, that is a clot, sealing your vessels. Monocytes interact with the clot, this led an inflammatory response that in our lab we are interested in. Led to Associated inflammatory response with the clot.\n
  • For those unfamiliar with the coagulation process i will try to explained in a simple way. First you have your blood in vessels and all its components like RBC, WBC, Platelets, Coagulations Factors: importants ones: Thombin and Fibrinogen. > When you get and injury in one of your vessels which happens many times each day, platelets recognizes some of the component in the subendothelial exposed layer and they aggregated forming a platelet plug, then they start secreting coagulation factors that leds to the activation of Thombin. Thrombin causes polymerization of fibrinogen molecules into fibrin fibers forming a meshwork that entraps blood cells, platelets and plasma, that is a clot, sealing your vessels. Monocytes interact with the clot, this led an inflammatory response that in our lab we are interested in. Led to Associated inflammatory response with the clot.\n
  • For those unfamiliar with the coagulation process i will try to explained in a simple way. First you have your blood in vessels and all its components like RBC, WBC, Platelets, Coagulations Factors: importants ones: Thombin and Fibrinogen. > When you get and injury in one of your vessels which happens many times each day, platelets recognizes some of the component in the subendothelial exposed layer and they aggregated forming a platelet plug, then they start secreting coagulation factors that leds to the activation of Thombin. Thrombin causes polymerization of fibrinogen molecules into fibrin fibers forming a meshwork that entraps blood cells, platelets and plasma, that is a clot, sealing your vessels. Monocytes interact with the clot, this led an inflammatory response that in our lab we are interested in. Led to Associated inflammatory response with the clot.\n
  • For those unfamiliar with the coagulation process i will try to explained in a simple way. First you have your blood in vessels and all its components like RBC, WBC, Platelets, Coagulations Factors: importants ones: Thombin and Fibrinogen. > When you get and injury in one of your vessels which happens many times each day, platelets recognizes some of the component in the subendothelial exposed layer and they aggregated forming a platelet plug, then they start secreting coagulation factors that leds to the activation of Thombin. Thrombin causes polymerization of fibrinogen molecules into fibrin fibers forming a meshwork that entraps blood cells, platelets and plasma, that is a clot, sealing your vessels. Monocytes interact with the clot, this led an inflammatory response that in our lab we are interested in. Led to Associated inflammatory response with the clot.\n
  • For those unfamiliar with the coagulation process i will try to explained in a simple way. First you have your blood in vessels and all its components like RBC, WBC, Platelets, Coagulations Factors: importants ones: Thombin and Fibrinogen. > When you get and injury in one of your vessels which happens many times each day, platelets recognizes some of the component in the subendothelial exposed layer and they aggregated forming a platelet plug, then they start secreting coagulation factors that leds to the activation of Thombin. Thrombin causes polymerization of fibrinogen molecules into fibrin fibers forming a meshwork that entraps blood cells, platelets and plasma, that is a clot, sealing your vessels. Monocytes interact with the clot, this led an inflammatory response that in our lab we are interested in. Led to Associated inflammatory response with the clot.\n
  • For those unfamiliar with the coagulation process i will try to explained in a simple way. First you have your blood in vessels and all its components like RBC, WBC, Platelets, Coagulations Factors: importants ones: Thombin and Fibrinogen. > When you get and injury in one of your vessels which happens many times each day, platelets recognizes some of the component in the subendothelial exposed layer and they aggregated forming a platelet plug, then they start secreting coagulation factors that leds to the activation of Thombin. Thrombin causes polymerization of fibrinogen molecules into fibrin fibers forming a meshwork that entraps blood cells, platelets and plasma, that is a clot, sealing your vessels. Monocytes interact with the clot, this led an inflammatory response that in our lab we are interested in. Led to Associated inflammatory response with the clot.\n
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  • Platelets Pour Plasma \n
  • Recently in our lab, using an in vitro clot system in which monocytes are exposed to clot removing the platelets, an increased in protein synthesis have been identified.\n
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  • My colaboration for the summer is the evaluation of the gene expression/ The goal of my 6 weeks project\n
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  • First 3 columns n=6\nLast 2 columns n=2\n
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  • Presentation summer 2011

    1. 1. MCP-1 AND IL-8 EXPRESSION BY MONOCYTES IN CLOTS Paola A. Arias-Mendoza, MS1 University of Puerto Rico, Medical Sciences Campus Robert Campbell, Ph.D. Andrew S. Weyrich, Ph.D. Program in Molecular Medicine University of Utah
    2. 2. INTRODUCTION• Cardiovascular Disease is the leading cause of death in US. • Arterial thrombosis – Myocardial infarction and stroke accounted for 37% of all deaths in 2007. • Venous thrombosis – Effects more than a million a year with 30% mortality within the first 30 days.
    3. 3. COAGULATION Red Blood Cell Leukocytes Platelets Coagulation Factors Thrombin (IIa) Fibrinogen
    4. 4. COAGULATION Red Blood Cell Leukocytes Platelets Coagulation Factors Thrombin (IIa) Fibrinogen
    5. 5. COAGULATION Red Blood Cell Leukocytes Platelets Coagulation Factors Thrombin (IIa) Fibrinogen
    6. 6. COAGULATION Red Blood Cell Leukocytes Platelets Coagulation Factors Thrombin (IIa) Fibrinogen
    7. 7. COAGULATION Red Blood Cell Leukocytes Platelets Coagulation Factors Thrombin (IIa) Fibrinogen
    8. 8. COAGULATION Red Blood Cell Leukocytes Platelets Coagulation Factors Thrombin (IIa) Fibrinogen
    9. 9. COAGULATION Red Blood Cell Leukocytes Platelets Coagulation Factors Thrombin (IIa) Fibrinogen
    10. 10. COAGULATION Red Blood Cell Leukocytes Platelets Coagulation Factors Thrombin (IIa) Fibrinogen
    11. 11. COAGULATION Red Blood Cell Leukocytes Platelets Coagulation Factors Thrombin (IIa) Fibrinogen
    12. 12. COAGULATION Red Blood Cell Leukocytes Platelets Coagulation Factors Thrombin (IIa) Fibrinogen
    13. 13. COAGULATION Red Blood Cell Leukocytes Platelets Coagulation Factors Thrombin (IIa) Fibrinogen
    14. 14. COAGULATION Red Blood Cell Leukocytes Platelets Coagulation Factors Thrombin (IIa) Fibrinogen
    15. 15. COAGULATION Red Blood Cell Leukocytes Platelets Coagulation Factors Thrombin (IIa) Fibrinogen
    16. 16. COAGULATION Red Blood Cell Leukocytes Platelets Coagulation Factors Thrombin (IIa) Fibrinogen
    17. 17. COAGULATION Red Blood Cell Leukocytes Platelets Coagulation Factors Thrombin (IIa) Fibrinogen
    18. 18. COAGULATION Red Blood Cell Leukocytes Platelets Coagulation Factors Thrombin (IIa) Fibrinogen
    19. 19. COAGULATION Red Blood Cell Leukocytes Platelets Coagulation Factors Thrombin (IIa) Fibrinogen
    20. 20. COAGULATION Red Blood Cell Leukocytes Platelets Coagulation Factors Thrombin (IIa) Fibrinogen
    21. 21. COAGULATION Red Blood Cell Leukocytes Platelets Coagulation Factors Thrombin (IIa) Fibrinogen
    22. 22. COAGULATION Red Blood Cell Leukocytes Platelets Coagulation Factors Thrombin (IIa) Fibrinogen
    23. 23. COAGULATION Red Blood Cell Leukocytes Platelets Coagulation Factors Thrombin (IIa) Fibrinogen
    24. 24. COAGULATION Red Blood Cell Leukocytes Platelets Coagulation Factors Thrombin (IIa) Fibrinogen
    25. 25. COAGULATION Red Blood Cell Leukocytes Platelets Coagulation Factors Thrombin (IIa) Fibrinogen
    26. 26. COAGULATION Red Blood Cell Leukocytes Platelets Coagulation Factors Thrombin (IIa) Fibrinogen
    27. 27. COAGULATION Red Blood Cell Leukocytes Platelets Coagulation Factors Thrombin (IIa) Fibrinogen
    28. 28. COAGULATION Red Blood Cell Leukocytes Platelets Coagulation Factors Thrombin (IIa) Fibrinogen
    29. 29. COAGULATION Red Blood Cell Leukocytes Platelets Coagulation Factors Thrombin (IIa) Fibrinogen
    30. 30. BACKGROUND• Activated platelets interact with monocytes promoting chemokine secretion involved in inflammatory response. (A.S. Weyrich 1996) • Monocyte Chemotactic Protein 1 (MCP-1) • Released by monocytes after induction by RANTES and P-selectin expressed by platelets. • Interleukin-8 (IL-8) also “neutrophil chemotatic factor” • Known chemokine and angiogenic factor• IL-8 and MCP-1 both needed for migration and proliferation of leukocytes in subendothelial layers (Z. Franks 2010)• IL-8 and MCP-1 have been found elevated in patients with stroke ( Z. Franks 2010)
    31. 31. IN VITRO SYSTEM Form clot from donors plasma Add 20 mM CaCl2 and tissue factorMonocytes
or
Monocytes
+
 Allow clot to form for 30 minutes platelets Overlay clot with monocytes or Plasma
Clot monocytes with platelets Collect supernatant at designated times and analyze for different cytokines
    32. 32. BACKGROUND 120000 100000 S Counts per Minutes 80000• Plasma Clots promotes an increase in the synthesis of 60000 proteins. 40000 35 20000 0 NT IIa LPS Clot
    33. 33. RESULTSIL‐8 MCP‐1 DAPI
=
Blue,
CD14
=
Green,
IL‐8
or
MCP‐1
=
RED
    34. 34. OBJECTIVE• Characterize MCP-1 and IL-8 mRNA expression in monocytes associated with clots.
    35. 35. MATERIALS AND METHODS• Blood peripheral monocytes isolation• In vitro Clot system experiments• RNA Isolation• cDNA Preparation• Real Time PCR
    36. 36. RESULTS
    37. 37. SUMMARY• MCP-1 and IL-8 mRNA and protein are increased in monocytes associated with clots• Our data suggest clinicians should consider the inflammatory consequences of thrombi in patients. Treatments focused on the inflammatory response may results in better outcomes.• Future Direction: • Identification of other chemokines and other inflammatory genes. • Determine mechanism of how clots induce inflammatory response.
    38. 38. ACKNOWLEDGEMENTS• PI: Andrew S. Weyrich, Ph.D.• Mentor: Robert A. Campbell, Ph.D.• Medical Research Program At University of Utah • Janet Basset• “Summer Family”
    39. 39. ACKNOWLEDGEMENTS• PI: Andrew S. Weyrich, Ph.D.• Mentor: Robert A. Campbell, Ph.D.• Medical Research Program At University of Utah • Janet Basset• “Summer Family”

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