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Phytochemical Screening and Evaluation of In-Vitro
Antioxidant and In-Vivo Hepatoprotective Activity
of the Urtica dioica Plant Extract
• Ranjit Pandey
• Contact- 00977-9849464685
• Kathmandu Nepal, CiST college
• In vitro comes from the Latin term "in glass" and refers to
studies of biological properties that are done in a test tube (i.e.
under glass. As opposed to in vivo ("in life") studies which are
done inside an organism.
• Himalayan range of Nepal has vast plant diversity from south
59m to north 8848m which contain wide important to
supplement modern allopathic medicine as well as traditional
believe of ethno medicinal therapy.
• Majority of Nepalese population still depends upon
indigenous use of medicinal plants and play vital role in
health and economy.
• Phytochemical screening is the qualitative analysis of plant
chemical constituents like alkaloids, carbohydrates, phenols,
flavonoid, tannins etc.
• Antioxidants may offer resistance against oxidative stress by
scavenging free radicals, inhibiting lipid peroxidation and by
other mechanisms and thereby help in preventing the free
radical induced liver diseases. Modern medicine have little to
offer alleviation of hepatic diseases and it is chiefly plant
based preparation which are employed for their treatment of
• Liver diseases are one of the major health problems in the
world caused by toxic chemicals, autoimmune disorders,
infections and excess consumption of alcohol.
• Hepato toxins inhibits anti-oxidation system like superoxide
dismutase (SOD), tissue glutathione (GSH) etc. and increases
the various liver enzymes (SGPT, SGOT, ALP, serum
bilirubin, cholesterol etc.) indicate liver injury.
Rationale of the study
• U. dioica has some etnnomedicinal uses including liver tonic.
• Literature review suggest very few researches have been
conducted on this plant.
• More than that research on its clinical use has not been found
• That encourages us to conduct the project on this plant. All of
these reasons inspire us to conduct the research on this plant.
Statement of the problem
• Hepatic disorders are common now a days due to different
xenobiotic and chemicals.
• No more allopathic medicine are available for liver disorder.
• Treatment of infection with liver disorder(e.g. jaundice) is
To perform Phytochemical Screening and Evaluation of In-
Vitro Antioxidant and In-Vivo Hepatoprotective activity.
The specific objectives of the present research project are to :
Collect and identify the proposed plant
Prepare and preserve herbaria and the authentic sample of
the collected plants .
Perform the phytochemical screening of sample
Perform the 50% ethanolic extraction of the collected samples
Evaluate antioxidant and hepatoprotactive activities of the
extract of collected samples
1. Collection and identification of crude drugs
2. Extraction of crude materials
3. Phytochemical screening:
Detection of alkaloids:
Detection of carbohydrates
Detection of glycosides
Detection of saponins
Detection of phytosterols
Detection of fixed oils & fats
Detection of resins
Detection of phenols.
Detection of tannins
Detection of flavonoids
Detection of proteins and aminoacids
Detection of diterpenes
Invivo: hepatoprotactive in wister albino rats.
Data will be reported as mean ± SEM. Statistical analysis of data
will be carried out by one way ANOVA comparing all test groups
versus control using graph-pad software.
Limitations of the study
• While phytochemical screening some compound present in
very trace amount cannot be detected by simple laboratory
reaction and advanced equipmet necessary which cannot be
performed in our laboratory.
• Discontinuation of electrical supply may interfere with the
Program Schedule Weak 1-5 Weak 6-10 Weak 11-15 Weak 16-20 Weak 21-25
drying of plant
1. Scientific outcomes
• To design new drug with potential pharmacological activity.
• This will provide a great contribution to the scientific field and
provides great help in the further study of these plants.
2. Social outcome
In Nepalese society, there is less awareness about these plant
species among the people. Many of them do not know about the
medicinal uses of these plants. After the completion of the
research project, people will be interested in the cultivation,
collection and conversion of these species
3. Economic outcomes
• In developing countries like Nepal, such studies will help in
the development of economic status and such unexplored
medicines will be utilized for the improvement of health
status of people.
Total expenditure of the project work is estimated as follows:
S. No. Particulars Cost (NRs.)
1. Chemicals and reagents 15000.00
2 Solvents 3000.00
3 Albino rats 4000.00
4 Rat diet 3000.00
5 Crude drugs 1500.00
6 Correspondences 2000.00
7 Books and journals 1000.00
8 Training (mouse handling) 5000.00
9 Printing and publishing 2000.00
10 Miscellaneous 2000.00
• 1. Gaire B. P. and S. L.;, Medicinal Plant Diversity and their Pharmacological
Aspects of Nepal Himalayas. Pharmacognosy Journal, 2011. 3(25).
• 2. Chhetri H. P., et al., PHYTOCHEMICAL AND ANTIMICROBIAL EVALUATIONS OF
SOME MEDICINAL PLANTS OF NEPAL. KATHMANDU UNIVERSITY JOURNAL OF
SCIENCE, ENGINEERING AND TECHNOLOGY, 2008. 1(5): p. 49-54.
• 3. Tiwari P., et al., Phytochemical screening and Extraction: A Review.
INTERNATIONALE PHARMACEUTICASCIENCIA, 2011. 1(1).
• 4. Qureshi M. N., et al., In-vitro Antioxidant and In-vivo Hepatoprotective
activity of Leucas ciliata leaves. Records of Natural Products
• 2010. 4(2): p. 124-130.
• 5. Shashank T., et al., Evaluation of Hepatoprotective activity of Stem Bark of
Homalium zeylanicum in Rats. International Journal of PharmTech Research, 2011.
3(3): p. 1630-1634.
• 6. Kumar P., et al., anti-oxident and hepatoprotective activity of tubers of