CELL INJURY, ADAPTATION,       and DEATH
Normal cell is in a steady state“Homeostasis”Change in Homeostasis due to stimuli -InjuryInjury - Reversible / Irrevers...
CELLULAR ADAPTATION TO STRESS    Adaptations are reversible changes in the number, size, phenotype,      metabolic activit...
Hypertrophy•is an increase in the size of cells & consequently an increase in thesize of an organ.•the enlargement is due ...
Physiologic Hypertrophy of the Uterus During Pregnancy   Gravid Uterus                   Normal Uterus
Small spindle-shaped uterine   Large, plump hypertrophiedsmooth muscle cells from a     smooth muscle cells from anormal u...
Heart hypertrophy inhypertension
Hyperplasia•is an increase in the number of cells in an organ or tissue•an adaptive response in cells capable of replicati...
HYPERPLASIA OF THE PROSTATE GLAND
Atrophy• Shrinkage in the size of the cell by the loss of cell substance•Results from decreased protein synthesis and incr...
Atrophy of the brain in an   Normal brain of a 25-year-old82-year-old man              man
ATROPHY IN OSTEOPOROSIS
Metaplasia•a reversible change in which one adult cell type ( epithelial ormesenchymal) is replaced by another adult cell ...
A.Schematic diagram of columnar to squamos epithelialB. Metaplastic transformation of esophageal epitheliumMesenchymal met...
METAPLASIA-ESOPHAGUS
METAPLASIA-LUNGS
CELLULAR INJURYCell Injury- pertains to the sequence of events when cells have no  adaptive response or the limits of adap...
Causes of Cell Injury1. Hypoxia   Causes:           a. Ischemia           b. Inadequate oxygenation of the blood          ...
5. Immunologic reactions   Example: anaphylactic reaction to a foreign protein or a drug            reaction to self antig...
MORPHOLOGY OF CELL AND TISSUE INJURY•   All stresses & noxious influences exert their effects first at the    molecular or...
PLASMA MEMBRANE ALTERATIONS                   •Cellular swelling                   •Formation of cytoplasmic              ...
Mithochondrial Changes              Early it appears condensed as a              result of loss of matrix protein         ...
Endoplasmic Reticulum Changes                                •Dilation                                •Detachment of      ...
Nuclear Alterations• Disaggregation of  granular and  fibrillar elements
Two Patterns of Morphologic Change Correlating toReversible Injury that can be recognized under the lightMicroscope: cellu...
Hydropic degeneration: kidneyCloudy swelling & hydropic change reflect failure of membrane ion pumps, due to lack of ATP, ...
Fatty Change* occurs in hypoxic injury & various forms of toxic( alcohol       & halogenated hydrocarbons like chloroform ...
Morphologic Alterations in Reversible Cell InjuryCell swelling•Fatty change•Plasma membrane blebbing and loss of microvill...
NECROSIS•Refers to a series of changes that accompany cell     death, largely resulting from the degradative action of    ...
Morphologic alterations in Necrosis✔ Increased eosinophilia (pink staining from eosin dye)✔ Myelin figures ( whorled phosp...
Patterns of Tissue NecrosisCoagulative Necrosis➢  A form of tissue necrosis in which the component cells aredead but the b...
Liquefactive Necrosis➢  Seen in focal bacterial or occassionally fungal infections      because microbes stimulate the acc...
Liquefactive necrosis. An infarct in the brain, showingdissolution of tissue
Caseous Necrosis➢   Encountered most often infoci of tuberculous infection➢  Characterized by a cheesy yellow-white appear...
Fat Necrosis➢  Refers to focal areas of fat destruction, typically resulting     from release of activated pancreatic lipa...
Fibrinoid necrosis➢  A special form of necrosis usually seen in immune      reactions involving blood vessels➢  This patte...
Gangrenous Necrosis➢   This is not a distinctive pattern of cell death➢  It is usually applied to a limb, generally the lo...
SUBCELLULAR RESPONSES TO INJURY•   Autophagy•Refers to lysosomal digestion of the cells owncomponents•It is thought to be ...
•   Hypertrophy of Smooth Endoplasmic ReticulumCells exposed to toxins that are metabolized in the SER show  hypertrophy, ...
General Principles Relevant To Most Forms Of Cell Injury• The cellular response to injurious stimuli depends on the type o...
MECHANISMS OF CELL INJURY➢  ATP depletion: failure of energy-dependent functions  reversible Injury                 necros...
Accumulation of Oxygen-Derived Free radicals (Oxidative  Stress)Free radicals are chemical species with single unpaired el...
Mechanisms that remove Free radicals● Action of superoxide dismutases (SODS)● Glutathione (GSH) peroxidase● Catalase prese...
APOPTOSIS (“FALLING OFF”)➢  Is a pathway of cell death that is induced by a tightly        regulated suicide program in wh...
Causes of ApoptosisApoptosis in Physiologic Situations        Death by apoptosis is a normal phenomenon thatserves to elim...
Death of cells that have served their useful purpose, suchas neutrophils in an acute inflammatory response andLymphocytes...
Apoptosis in Pathologic Situations        Apoptosis eliminates cells that are genetically altered orInjured beyond repair ...
    Cell injury in certain infections particularly viral infectionsPathologic atrophy in parenchymal organs after ductob...
Two Major Pathways in the Initiation of Apopotosis1) Mitochondrial ( intrinsic) pathway       Triggered by loss of surviva...
INTRACELLULAR ACCUMULATIONSTHREE MAIN PATHWAYS OF ABNORMAL INTRACELLULARACCUMULATIONS● A normal substance is produced at a...
Fatty Change (Steatosis)Refers to any abnormal accumulation of triglycerides within✔   parenchymal cellsMost often seen in...
Fatty Liver
Cholesterol and Cholesteryl Esters✔    Result of defective catabolism and excessive intakePresent in lipid vacoules of smo...
Proteins✔  Less common than lipid accumulations✔  Occur because excess are presented to the cells or     because the cells...
Protein reabsorption droplets in the renal tubular epithelium
Glycogen✔ Accumulations of these are associated with abnormalities     in the metabolism of either glucose or glycogen✔ Ex...
Pigments➢  colored substances that are either exogenous or      endogenous●  Exogenous – coming from outside the body     ...
●  Endogenous – synthesized within the body itself       1) Lipofuscin or “wear-and -tear pigment or lipochrome✔ an insolu...
The pale golden brown finely granular pigment seen here in nearlyall hepatocytes is lipochrome (lipofuscin).
2) Melanin✔ An endogenous, brown-black pigment synthesized     exclusively by melanocytes when the enzyme tyrosinase     c...
Melanin pigment in melanoma
3) Hemosiderin✔  A hemoglobin-derived granular pigment that is     golden yellow to brown and accumulates in tissues     w...
Local excess of iron & consequently of hemosiderin result    ✔     from hemorrhage     Ex. Bruise       The original red-b...
Hemosiderosis✔ a condition where hemosiderin is deposited in many      organs and tissues whenever there is systemic overl...
PATHOLOGIC CALCIFICATION➢  implies the abnormal deposition of calcium salts, together   with small amounts of iron, magnes...
2) Tissue undergoing slow degeneration● Hyaline areas in benign tumors● Fibroids● In arteries due to atheromatous degenera...
B. Metastatic Calcification✔  deposition of calcium salts in normal tissues✔ almost always reflects some derangement in ca...
CELLULAR AGING➢  results from combination of accumulating cellular damage  (e.g., by free radicals), reduced capacity to d...
●  Replicative senescence✔ Reduced capacity of cells to divide because of decreasing     amounts of telomerase and progres...
Telomerase in Ageing:GermcellsSomaticcells
WERNER SYNDROME➢ A rare disease characterized by premature aging
Cell injury, adaptation, and death fix
Cell injury, adaptation, and death fix
Cell injury, adaptation, and death fix
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Cell injury, adaptation, and death fix

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Cell injury, adaptation, and death fix

  1. 1. CELL INJURY, ADAPTATION, and DEATH
  2. 2. Normal cell is in a steady state“Homeostasis”Change in Homeostasis due to stimuli -InjuryInjury - Reversible / IrreversibleAdaptation / cell death
  3. 3. CELLULAR ADAPTATION TO STRESS Adaptations are reversible changes in the number, size, phenotype, metabolic activity or functions of cells in response to changes in their environment• Physiologic adaptations are responses of cells to normal stimulation by hormones or endogenous chemical mediators• Pathologic adaptations are responses to stress that allow cells to modulate their structure and function and thus escape injury
  4. 4. Hypertrophy•is an increase in the size of cells & consequently an increase in thesize of an organ.•the enlargement is due to an increased synthesis of structural proteins & organelles•Occurs when cells are incapable of dividing Types: a) physiologic b) pathologic Causes: a) increased functional demand b) hormonal stimulation
  5. 5. Physiologic Hypertrophy of the Uterus During Pregnancy Gravid Uterus Normal Uterus
  6. 6. Small spindle-shaped uterine Large, plump hypertrophiedsmooth muscle cells from a smooth muscle cells from anormal uterus gravid uterus
  7. 7. Heart hypertrophy inhypertension
  8. 8. Hyperplasia•is an increase in the number of cells in an organ or tissue•an adaptive response in cells capable of replication•a critical response of connective tissue cells in wound healing Types: a) physiologic hyperplasia 1) hormonal ex. Proliferation of glandular epithelium of the female breast at puberty & during pregnancy 2) compensatory – hyperplasia that occurs when a portionof a tissue is removed or diseased e.g. partial resection of a liver > mitotic activity 12 hours later b) pathologic hyperplasiaCaused by excessive hormonal or growth factor• stimulation
  9. 9. HYPERPLASIA OF THE PROSTATE GLAND
  10. 10. Atrophy• Shrinkage in the size of the cell by the loss of cell substance•Results from decreased protein synthesis and increased protein degradation in cells•Is accompanied in many situations by increased autophagy with resulting Increases in autophagic vacoules Causes:• Decreased workload• Loss of innervation• Diminished blood supply• Inadequate nutrition• Loss of endocrine stimulation• Aging (senile atrophy)
  11. 11. Atrophy of the brain in an Normal brain of a 25-year-old82-year-old man man
  12. 12. ATROPHY IN OSTEOPOROSIS
  13. 13. Metaplasia•a reversible change in which one adult cell type ( epithelial ormesenchymal) is replaced by another adult cell type.• is cellular adaptation whereby cells sensitive to a particular stress are replaced by other cell types better able to withstand the adverse environmentEpithelial metaplasia Examples• Squamos change that occurs in the respiratory epithelium in habitual cigarette smokers ( normal columnar epithelial cells of trachea & bronchi are replaced by stratified squamos epithelial cells• Vitamin A deficiency• Chronic gastric reflux, the normal stratified squamos epithelium of the lower esophagus may undergo metaplasia to gastric columnar epithelium
  14. 14. A.Schematic diagram of columnar to squamos epithelialB. Metaplastic transformation of esophageal epitheliumMesenchymal metaplasia Ex. Bone formed in soft tissue particularly in foci of injury
  15. 15. METAPLASIA-ESOPHAGUS
  16. 16. METAPLASIA-LUNGS
  17. 17. CELLULAR INJURYCell Injury- pertains to the sequence of events when cells have no adaptive response or the limits of adaptive capability are exceededTypes of Cell Injury1. Reversible Injury- injury that persists within certain limits, cells return to a stable baseline2. Irreversible Injury- when the stimulus causing the injury persists and is severe enough from the beginning that the affected cells die a. necrosis b. apoptosis
  18. 18. Causes of Cell Injury1. Hypoxia Causes: a. Ischemia b. Inadequate oxygenation of the blood c. Reduction in the oxygen-carrying capacity of the blood2. Chemical Agents a. glucose, salt or oxygen b. poisons c. environmental toxins d. social “stimuli” e. therapeutic drugs3. Physical agents- trauma, extremes of temperature, radiation, electric shock, & sudden changes in atmospheric pressure4. Infectious agents
  19. 19. 5. Immunologic reactions Example: anaphylactic reaction to a foreign protein or a drug reaction to self antigens6. Genetic defects Examples are genetic malformations associated with Down Syndrome, sickle cell anemia & inborn errors of metabolism7. Nutritional Imbalances
  20. 20. MORPHOLOGY OF CELL AND TISSUE INJURY• All stresses & noxious influences exert their effects first at the molecular or biochemical level• Cellular function is lost far before cell death occurs and the morphologic changes of cell injury (or death) lag far behind both• Ultrastructural Changes of Reversible Cell injury• Alteration in plasma membrane reflecting disturbance in ion and volume regulation induced by loss of ATP2. Mitochondrial changes3. Endoplasmic reticulum changes4. Nuclear alterations
  21. 21. PLASMA MEMBRANE ALTERATIONS •Cellular swelling •Formation of cytoplasmic blebs •Blunting and distortion of microvilli •Deterioration and loosening of intercellular attachments
  22. 22. Mithochondrial Changes Early it appears condensed as a result of loss of matrix protein following loss of ATP Followed by swelling due to ionic shifts Amorphous densities which correlate with the onset of irreversibility Finally, rupture of membrane followed by progressing increased calcification
  23. 23. Endoplasmic Reticulum Changes •Dilation •Detachment of ribosomes and dissociation of polysomes with decreased protein synthesis •Progressive fragmentation and formation of intracellular aggregates of myelin figures
  24. 24. Nuclear Alterations• Disaggregation of granular and fibrillar elements
  25. 25. Two Patterns of Morphologic Change Correlating toReversible Injury that can be recognized under the lightMicroscope: cellular swelling and fatty changeCellular Swelling● Is the result of failure of energy-dependent ion pumps in the plasma membrane leading to an inability to maintain ionic & fluid homeostasis● first manifestation of almost all forms of injury to cells•microscopically small, clear vacoules may be seen within the cytoplasm•sometimes called hydropic change or vacoular degeneration•swelling of cells is reversible
  26. 26. Hydropic degeneration: kidneyCloudy swelling & hydropic change reflect failure of membrane ion pumps, due to lack of ATP, allowing cells to accumulate fluid
  27. 27. Fatty Change* occurs in hypoxic injury & various forms of toxic( alcohol & halogenated hydrocarbons like chloroform ) or metabolic injury like diabetes mellitus & obesity •manifested by the appearance of lipid vacoules in the cytoplasm •principally encountered in cells participating in and involved in fat metabolism e.g. hepatocytes & myocardial cells •also reversible
  28. 28. Morphologic Alterations in Reversible Cell InjuryCell swelling•Fatty change•Plasma membrane blebbing and loss of microvilli•Mitochondrial swelling•Dilation of the ER•Eosinophilia (due to decreased cytoplasmic RNA)•
  29. 29. NECROSIS•Refers to a series of changes that accompany cell death, largely resulting from the degradative action of enzymes on lethally injured cells•The enzymes responsible for digestion of the cell are derived either from the: 1) Lysosomes of the dying cells themselves or from 2) lysosomes of leukocytes that are recruited as part of the inflammatory reaction to the dead cells
  30. 30. Morphologic alterations in Necrosis✔ Increased eosinophilia (pink staining from eosin dye)✔ Myelin figures ( whorled phospholipid masses)✔ Nuclear changes assume one of three patterns all due to breakdown of DNA & chromatin: 1) Karyolysis 2) Pyknosis characterized by nuclear shrinkage and increased basophila 3) Karyorrhexis – fragmentation and dissolution✔ Breakdown of plasma membrane and organellar Membranes✔ Leakage and enzymatic digestion of cellular contents
  31. 31. Patterns of Tissue NecrosisCoagulative Necrosis➢ A form of tissue necrosis in which the component cells aredead but the basic tissue architecture is preserved for at least several days➢ It is characteristics of infarcts ( areas of ischemic necrosis) in all solid organs except the brain A wedge-shaped kidney Infarct (yellow) with preserva tion of the outlines
  32. 32. Liquefactive Necrosis➢ Seen in focal bacterial or occassionally fungal infections because microbes stimulate the accumulation of Inflammatory cells and the enzymes of leukocytes digest ( “liquefy”) the tissue➢ This necrosis is characteristic of hypoxic death of cells witnin the CNS➢ Associated with suppurative inflammation (accumulation of pus)➢ The areas undergoing necrosis are transformed into a Semi-solid consistency or state (liquid viscuous mass) Example: abcess
  33. 33. Liquefactive necrosis. An infarct in the brain, showingdissolution of tissue
  34. 34. Caseous Necrosis➢ Encountered most often infoci of tuberculous infection➢ Characterized by a cheesy yellow-white appearance of the area of necrosis➢ It is often enclosed within a distinctive inflammatory border A tuberculous lung with a large area of caseous necrosis containing yellow-white and cheesy debris
  35. 35. Fat Necrosis➢ Refers to focal areas of fat destruction, typically resulting from release of activated pancreatic lipases into the substance of the pancreas and the peritoneal cavity➢ Occurs in acute pancreatitis Fat necrosis in aqcute pancreatitis. The areas of white chalky deposits represent foci of fat necrosis with calcium soapformation (saponification) at sites of lipid breakdown in the mesentery
  36. 36. Fibrinoid necrosis➢ A special form of necrosis usually seen in immune reactions involving blood vessels➢ This pattern of necrosis is prominent when complexes of antigens and antibodies are deposited in the walls of Arteries➢ Deposits of these immune complexes together with fibrin that has leaked out of vessels result in a bright pink and amorphous appearance called fibrinoid” Fibrinoid necrosis in an artery in a patient with Polyarteritis Nodosa. The wall of the artery shows a circumferential bright pink area of necrosis with protein deposition and inflammation
  37. 37. Gangrenous Necrosis➢ This is not a distinctive pattern of cell death➢ It is usually applied to a limb, generally the lower leg, that has lost its blood supply involving multiple tissue layers➢ Types:✔ Wet gangrene✗ Occurs in naturally moist areas like mouth, bowels lungs✗ Characterized by numerous bacteria✔ Dry gangrene✗ begins at the distal part of the limb due to ischemia andoften occurs in the toes and feet of elderly patients due to arteriosclerosis✗ This is mainly due to arterial occlusion✗ There is limited putrefaction and bacteria fail to survive
  38. 38. SUBCELLULAR RESPONSES TO INJURY• Autophagy•Refers to lysosomal digestion of the cells owncomponents•It is thought to be a survival mechanism in times of nutrient deprivation•Organelles are enclosed in vacoules that fuse with lysosomes Heterophagy a cell usually a macrophage ingests substances from the outside for intracellular destruction
  39. 39. • Hypertrophy of Smooth Endoplasmic ReticulumCells exposed to toxins that are metabolized in the SER show hypertrophy, a compensatory mechanism to maximize removal of the toxins• Mitochondrial Alterations * alterations in size, number, shape & function Ex. Mitochondria assume extremely large & abnormal shapes (megamitochondria) in hepatocytes in various nutritional deficiencies & alcoholic liver disease Cellular hypertrophy > # of mitochondria in cells Atrophy < # of mitochondria• Cytoskeletal Abnormalities some drugs & toxins interfere with the assembly & functions of Cytoskeleton filaments or result in abnormal accumulations of filaments
  40. 40. General Principles Relevant To Most Forms Of Cell Injury• The cellular response to injurious stimuli depends on the type of injury, its duration, and its severity• The consequences of an injurious stimulus depend on the type , status , adaptability , and genetic makeup of the injured cell•Cell injury results from functional & biochemical abnormalities in• one or more of several essential cellular components The most important target of injurious stimuli are: 1) cell membrane integrity, critical to cellular ionic and osmotic homeostasis 2) mitochondrial, the site of adenosine triphosphate (ATP) generation 3) protein synthesis 4) integrity of the genetic apparatus 5) cytoskeleton
  41. 41. MECHANISMS OF CELL INJURY➢ ATP depletion: failure of energy-dependent functions reversible Injury necrosis➢ Mitochondrial damage: ATP depletion failure of energy- dependent cellular functions ultimately necrosis; under some conditions, leakage of proteins that causes apoptosis➢ Influx of calcium: activation of enzymes that damage cellular components and may also trigger apoptosis➢ Accumulation of reactive oxygen species: covalent modifications of cellular proteins, lipids, nucleic acids➢ Increased permeability of cellular membranes: may affect plasma membrane, lysosomal membranes, mitochondrial membranes; typically culminates in necrosis➢ Accumulations of damaged DNA and misfolded proteins triggers apoptosis
  42. 42. Accumulation of Oxygen-Derived Free radicals (Oxidative Stress)Free radicals are chemical species with single unpaired electron in an outer orbital. In such a state the radicals are extremely unstable & readily react with inorganic or organic chemicals. Free radicals may be generated within cells by• Reduction-oxidation (redox) reactions• Nitric oxide (NO)• Absorption of radiant energy (e.g. ultraviolet light, x-rays)• Enzymatic metabolism of exogenous chemicals (e.g. carbon tetrachloride)• Inflammation, because free radicals are produced by leukocytes that enter tissues
  43. 43. Mechanisms that remove Free radicals● Action of superoxide dismutases (SODS)● Glutathione (GSH) peroxidase● Catalase present in perixisomes● Endogenous or exogenous antioxidants (e.g. vitamins E, A and C, and beta-Carotene may either block the formation of free radicals or scavenge them once they have formed● Iron and Copper can catalyze the formation of Reactive Oxygen Species (ROS)
  44. 44. APOPTOSIS (“FALLING OFF”)➢ Is a pathway of cell death that is induced by a tightly regulated suicide program in which cells destined to die activate enzymes capable of degrading the cells own nuclear DNA and nuclear and cytoplasmic proteins➢ It differs from necrosis in the following characteristics 1) Plasma membrane of the apoptotic cell remains intact 2) Has no leakage of cellular contents 3) Does not elicit an inflammatory reaction in the host➢ Sometimes coexist with necrosis➢ Apoptosis induced by some pathologic stimuli may progress to necrosis
  45. 45. Causes of ApoptosisApoptosis in Physiologic Situations Death by apoptosis is a normal phenomenon thatserves to eliminate cells that are no longer needed and tomaintain a steady number of various cell populations in tissues Programmed destruction of cells during embryogenesis, Including implantation, organogenesis, developmental involution, and metamorphosisInvolution of hormone- dependent tissues upon hormone deprivation such as endometrial cell breakdown during the menstrual cycle and regeression of the lactating breast after WeaningCell loss in proliferating cell populations, such as intestinalCrypt epithelia
  46. 46. Death of cells that have served their useful purpose, suchas neutrophils in an acute inflammatory response andLymphocytes at the end of an immune responseElimination of potentially harmful self-reactive lymphocytesEither before or after they have completed their maturationCell death induced by cytotoxic T lymphocytes, a defensemechanism against viruses and tumors that serves to killeliminate virus-infected and neoplastic cells
  47. 47. Apoptosis in Pathologic Situations Apoptosis eliminates cells that are genetically altered orInjured beyond repair without eliciting a severe host reaction,thus keeping the damage as contained as possible DNA damage Radiation, cytotoxic anticancer drugs, extremes of temperature and even hypoxia can damage DNA either directly or via production of free radicals Accumulation of misfolded proteins✔ These may arise because of mutations in the genes encoding these proteins or because of extrinsic factors such as free radicals✔ Excessive accumulation of these proteins in the ER leads to a condition called ER stress
  48. 48.  Cell injury in certain infections particularly viral infectionsPathologic atrophy in parenchymal organs after ductobstruction such as in pancreas, parotid gland and kidneyMorphologic Alterations in Apoptosis● Nuclear chromatin condensation● Formation of apoptotic bodies ( fragments of nuclei and cytoplasm)The fundamental event in apoptosis is the activation of enzymecalled caspases
  49. 49. Two Major Pathways in the Initiation of Apopotosis1) Mitochondrial ( intrinsic) pathway Triggered by loss of survival signals, DNA damage and accumulation of misfolded proteins (ER stress)2) Death receptor (extrinsic) pathway Responsible for the elimination of self-reactive lymphocytes and damage by cytotoxic T lymphocytes
  50. 50. INTRACELLULAR ACCUMULATIONSTHREE MAIN PATHWAYS OF ABNORMAL INTRACELLULARACCUMULATIONS● A normal substance is produced at abnormal or an increased rate, but metabolic rate is inadequate to remove it Example. Fatty change in the liver● A normal or abnormal endogenous substance accumulates because of genetic or acquired defects in its folding, packaging, transport or secretion Example. Accumulation of of proteins in anti-trypsin deficiency● An abnormal exogenous substance is deposited and Accumulates because the cell has neither the enzymatic Machinery to degrade the substance nor the ability to transport It to other sites. Example. Accumulation of carbon or silica particles
  51. 51. Fatty Change (Steatosis)Refers to any abnormal accumulation of triglycerides within✔ parenchymal cellsMost often seen in the liver but may also occur in the heart,✔ Skeletal muscle, kidney and other organsMay be caused by toxins, protein malnutrition, diabetes✔ mellitus, obesity and anoxiaAlcohol abuse and diabetes associated with obesity are✔ the most common causes of fatty liver
  52. 52. Fatty Liver
  53. 53. Cholesterol and Cholesteryl Esters✔ Result of defective catabolism and excessive intakePresent in lipid vacoules of smooth muscle cells and✔ macrophages in atherosclerosis (hardening of the aorta)Give atherosclerotic plaques their characteristic yellow color✔ and contibute to the pathogenesis of the lesion✔ Xanthomas are hypercholesterolemic tumurous masses found in the connective tissue of the skin or tendons
  54. 54. Proteins✔ Less common than lipid accumulations✔ Occur because excess are presented to the cells or because the cells synthesize excessive amounts✔ Examples: 1) Nephrotic syndrome there is heavy protein leakage across the glomerular filter due to a much larger reabsorption of albumin 2) accumulation of newly synthesized imunoglobulins in RER of some plasma cells forming rounded, eosinophilic Russell bodies 3) Mallory body or “ alcoholic hyalin” is an eosinophilic cytoplasmic inclusion in liver cells highly characteristic of alcoholic liver disease 4) Neurofibrillary tangle found in the brain in Alzheimer disease
  55. 55. Protein reabsorption droplets in the renal tubular epithelium
  56. 56. Glycogen✔ Accumulations of these are associated with abnormalities in the metabolism of either glucose or glycogen✔ Ex. 1) In poorly controlled diabetes mellitus, glycogen accumulates in renal tubular epithelium, cardiac myocytes, and β cells of Islets of langerhans 2) Glycogen storage diseases or glycogeneses are Genetic disorders where glycogen accumulates in macrophages of patients with defects in lysosomal enzymes
  57. 57. Pigments➢ colored substances that are either exogenous or endogenous● Exogenous – coming from outside the body 1) Carbon ( ex. Coal dust)➔ Most common air pollutant➔ Aggregates of the pigment blacken the draining lymph nodes and pulmonary parenchyma (Anthracosis)➔ Heavy accumulations may induce emphysema or a fibroblastic reaction that can result in a serious lung disease called coal workers pneumoconiosis
  58. 58. ● Endogenous – synthesized within the body itself 1) Lipofuscin or “wear-and -tear pigment or lipochrome✔ an insoluble brownish-yellow granular intracellular material that accumulates in the heart, liver, & brain as a function of age or atrophy✔ represents complexes of lipid & protein that derive from the free radical-catalyzed peroxidation of polyunsaturated lipids✔ it is not injurious to the cell but is important as a marker of past free-radical injury✔ the brown pigment when present in large amounts, imparts an appearance to the tissue that is called brown atrophy
  59. 59. The pale golden brown finely granular pigment seen here in nearlyall hepatocytes is lipochrome (lipofuscin).
  60. 60. 2) Melanin✔ An endogenous, brown-black pigment synthesized exclusively by melanocytes when the enzyme tyrosinase catalyzes tyrosine to (DOPA) dihydroxyphenylalanine located in the epidermis✔ Acts as a screen against harmful ultraviolet radiation✔ Basal keratinocytes in the skin can accumulate the pigment (e.g. in freckles)
  61. 61. Melanin pigment in melanoma
  62. 62. 3) Hemosiderin✔ A hemoglobin-derived granular pigment that is golden yellow to brown and accumulates in tissues when there is a local or systemic excess of iron✔ Iron is normally stored within cells in association with the protein apoferritin, forming ferritin micelles✔ Iron can be identified by the Prussian blue reaction
  63. 63. Local excess of iron & consequently of hemosiderin result ✔ from hemorrhage Ex. Bruise The original red-blue color of hemoglobin is transformed to varying shades of green-blue by the local formation of biliverdin (green bile) and bilirubin (red bile) from the heme moiety✔ The iron of hemoglobin accumulate as golden- yellow hemosiderin
  64. 64. Hemosiderosis✔ a condition where hemosiderin is deposited in many organs and tissues whenever there is systemic overload of iron✔ It occurs in the following settings•Increased absorption of dietary iron•Impaired utilization of iron•Hemolytic anemias•Transfusions Hereditary Hemochromatosis✔ A condition where there is extensive accumulations of iron with tissue injury like liver fibrosis, heart failure and diabetes mellitus✔ Characterized principally by 1) the deposition of hemosiderin in the following organs (in decreasing order of severity):liver, pancreas, myocardium, pituitary, adrenal, thyroid, joints & skin 2) cirrhosis and 3) pancreatic fibrosis
  65. 65. PATHOLOGIC CALCIFICATION➢ implies the abnormal deposition of calcium salts, together with small amounts of iron, magnesium, and other mineralsTYPESA. Dystrophic calcification✔ deposition of calcium in dead or dying tissues✔ occurs in the absence of calcium metabolic derangements ( with normal serum levels of calcium)✔ Local deposits of calcium may occur in 1) necrotic tissue which is not absorbed● old infarcts● tuberculous foci● old collection of pus● dead parasites● acute pancreatic necrosis
  66. 66. 2) Tissue undergoing slow degeneration● Hyaline areas in benign tumors● Fibroids● In arteries due to atheromatous degeneration or old age● Old thrombi● Diseased or abnormal heart valvesPathogenesis➢ Initiation ( or nucleation)➢ Propagation Both may be intracellular or extracellular with calciumphosphate as the end product
  67. 67. B. Metastatic Calcification✔ deposition of calcium salts in normal tissues✔ almost always reflects some derangement in calcium metabolism ( hypercalcemia)Four major causes of hypercalcemia1) Increased secretion of parathyroid hormone, due to either parathyroid tumors or production of parathyroid hormone- Related protein by other malignant tumors2) Destruction of bone due to the effects of accelerated turnover (e.g. Paget disease),immobilization, or tumors (increased bone catabolism associated with multiple myeloma, leukemia or diffuse skeletal metastases)3) Vitamin D-related disorders like vitamin D intoxication and sarcoidosis ( in which macrophages activate a vitamin D precursor4) Renal failure, in which phosphate retention leads to secondary hyperparathyroidism
  68. 68. CELLULAR AGING➢ results from combination of accumulating cellular damage (e.g., by free radicals), reduced capacity to divide (replicative senescence), and reduced ability to repair damaged DNACellular senescence➢ Aging of a person is intimately related to cellular agingMechanisms known or suspected to be responsible for cellularaging● DNA damage✔ defective DNA repair mechanisms DNA repair may be activated by calorie restriction (known to prolong aging in model organisms)
  69. 69. ● Replicative senescence✔ Reduced capacity of cells to divide because of decreasing amounts of telomerase and progressive shortening of chromosomal ends (telomeres) Telomeres➔ are short repeated sequences of DNA present at the linear ends of chromosomes➔ Importance * for ensuring the complete replication of chromosome ends * and for protecting the ends from fusion & degradation● Progressive accumulation of metabolic damage✔ Repeated environmental exposure to radiation✔ Progressive reduction of antioxidant defense mechanism Like Vit. E & glutathione peroxidase● Possible roles of growth factors
  70. 70. Telomerase in Ageing:GermcellsSomaticcells
  71. 71. WERNER SYNDROME➢ A rare disease characterized by premature aging
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