Fat Metabolism I’m not fat, I’ve just got a lot of potential energy! www.freelivedoctor.com
Digestion, Mobilization, and Transport www.freelivedoctor.com
Emulsification <ul><li>Fats are not water soluble </li></ul><ul><li>Made into bile salts that are </li></ul><ul><li>Absorb...
Chylomicron Carriers <ul><li>proteins that carry fats </li></ul><ul><li>stored in adipose tissue </li></ul>www.freelivedoc...
Role of Hormones <ul><li>Can mobilize from adipose </li></ul><ul><li>Release as Fatty acids </li></ul><ul><li>Epinephrene ...
Animation of Process www.freelivedoctor.com
Activation and Transported to Mitochondria <ul><li>FA + CA --> ATP = FA-CoA  + AMP + Ppi </li></ul><ul><li>Driven by break...
Carnitine www.freelivedoctor.com
Formation of acyl carnitine <ul><li>Can get transported across inner mitochondrial membrane </li></ul>www.freelivedoctor.com
Animation of Process www.freelivedoctor.com
Beta Oxidation  <ul><li>Breakdown of fats into  </li></ul><ul><ul><li>Acetyl coenzyme A --> Kreb Cycle </li></ul></ul><ul>...
Reactions Diagram www.freelivedoctor.com
Reactions of Beta Oxidation  Acyl-CoA Dehydrogenase <ul><ul><li>Puts in a ß double bond </li></ul></ul><ul><ul><ul><li>Oxi...
Reactions of Beta oxidation Enoyl CoA Hydratase <ul><li>adds water </li></ul><ul><ul><li>forms ß-hydroxy product </li></ul...
Reactions of Beta Oxidation  ß-hydroxylacyl-CoA dehydrogenase <ul><li>oxidizes secondary alcohol to ketone </li></ul><ul><...
Reactions of Beta Oxidation  Acyl-CoA acetyltransferase (thiolase) <ul><li>Uses another CoA </li></ul><ul><li>Produces Ace...
Overall Per Beta Oxidation cycle <ul><li>1 FADH 2 …………………………1.5 ATP </li></ul><ul><li>1 NADH………………………….2.5 ATP </li></ul><...
Beta Oxidation on 16 C fatty Acid www.freelivedoctor.com
Beta Oxidation on 16 C fatty Acid <ul><li>7 rounds of Beta oxidation (bottom numbers) </li></ul><ul><li>Form 8 acetyl Co A...
Beta Oxidation on 16 C fatty Acid totals <ul><li>7 rounds of beta oxidation </li></ul><ul><ul><li>7 rounds  X  FADH 2  X  ...
Unsaturated Fat Metabolism <ul><li>special enzymes needed to convert cis bonds in fatty acids to trans bonds </li></ul>www...
Regulation of Fat Metabolism <ul><li>Transport is rate-limiting </li></ul><ul><li>Regulation of carnitine acyl transferase...
Ketone Bodies <ul><li>Starvation causes accumulation of acetyl CoA </li></ul><ul><ul><li>not enough carbohydrates to keep ...
Diabetes and Ketone Bodies <ul><li>When there is not enough insulin in the blood and it must break down fat for its energy...
Comparison of Fat Metabolism per gram and CHO per gram <ul><li>Fats provide about 9 kilocalories per gram and carbohydrate...
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Fatmetabolism

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Fatmetabolism

  1. 1. Fat Metabolism I’m not fat, I’ve just got a lot of potential energy! www.freelivedoctor.com
  2. 2. Digestion, Mobilization, and Transport www.freelivedoctor.com
  3. 3. Emulsification <ul><li>Fats are not water soluble </li></ul><ul><li>Made into bile salts that are </li></ul><ul><li>Absorbed as micelles in small intestines </li></ul>www.freelivedoctor.com
  4. 4. Chylomicron Carriers <ul><li>proteins that carry fats </li></ul><ul><li>stored in adipose tissue </li></ul>www.freelivedoctor.com
  5. 5. Role of Hormones <ul><li>Can mobilize from adipose </li></ul><ul><li>Release as Fatty acids </li></ul><ul><li>Epinephrene and glucagon release cAMP </li></ul><ul><ul><li>make fatty acids and glycerol </li></ul></ul><ul><ul><li>mobilizes fat </li></ul></ul>www.freelivedoctor.com
  6. 6. Animation of Process www.freelivedoctor.com
  7. 7. Activation and Transported to Mitochondria <ul><li>FA + CA --> ATP = FA-CoA + AMP + Ppi </li></ul><ul><li>Driven by breakup of Ppi </li></ul><ul><li>Acyl CoA Synthetase </li></ul><ul><li>-32.5 kJ </li></ul><ul><li>Transported through inner mito membrane via carnitine </li></ul><ul><ul><li>uses specific acyl carnitine transporter </li></ul></ul>www.freelivedoctor.com
  8. 8. Carnitine www.freelivedoctor.com
  9. 9. Formation of acyl carnitine <ul><li>Can get transported across inner mitochondrial membrane </li></ul>www.freelivedoctor.com
  10. 10. Animation of Process www.freelivedoctor.com
  11. 11. Beta Oxidation <ul><li>Breakdown of fats into </li></ul><ul><ul><li>Acetyl coenzyme A --> Kreb Cycle </li></ul></ul><ul><ul><li>FADH 2 --> Oxidative Phosphorylation </li></ul></ul><ul><ul><li>NADH--> Oxidative Phosphorylation </li></ul></ul><ul><li>Breaks off two carbons at a time to acetyl CoA </li></ul><ul><ul><li>Remaining goes another round </li></ul></ul>www.freelivedoctor.com
  12. 12. Reactions Diagram www.freelivedoctor.com
  13. 13. Reactions of Beta Oxidation Acyl-CoA Dehydrogenase <ul><ul><li>Puts in a ß double bond </li></ul></ul><ul><ul><ul><li>Oxidizes C-C bond to double bond </li></ul></ul></ul><ul><ul><li>Produces FADH 2 </li></ul></ul>www.freelivedoctor.com
  14. 14. Reactions of Beta oxidation Enoyl CoA Hydratase <ul><li>adds water </li></ul><ul><ul><li>forms ß-hydroxy product </li></ul></ul>www.freelivedoctor.com
  15. 15. Reactions of Beta Oxidation ß-hydroxylacyl-CoA dehydrogenase <ul><li>oxidizes secondary alcohol to ketone </li></ul><ul><ul><li>Oxygen-containing group </li></ul></ul><ul><ul><li>Produces NADH </li></ul></ul>www.freelivedoctor.com
  16. 16. Reactions of Beta Oxidation Acyl-CoA acetyltransferase (thiolase) <ul><li>Uses another CoA </li></ul><ul><li>Produces Acetyl CoA </li></ul><ul><li>Acyl CoA can go another round </li></ul>www.freelivedoctor.com
  17. 17. Overall Per Beta Oxidation cycle <ul><li>1 FADH 2 …………………………1.5 ATP </li></ul><ul><li>1 NADH………………………….2.5 ATP </li></ul><ul><li>1 Acetyl CoA to Krebs </li></ul><ul><ul><li>3 NADH X 2.5 ATP / NADH………7.5 ATP </li></ul></ul><ul><ul><li>1 FADH 2 …………………………….1.5 ATP </li></ul></ul><ul><ul><li>1 GTP……………………………….. 1.0 ATP </li></ul></ul><ul><ul><ul><ul><ul><li>Total = 14.0 ATP </li></ul></ul></ul></ul></ul>www.freelivedoctor.com
  18. 18. Beta Oxidation on 16 C fatty Acid www.freelivedoctor.com
  19. 19. Beta Oxidation on 16 C fatty Acid <ul><li>7 rounds of Beta oxidation (bottom numbers) </li></ul><ul><li>Form 8 acetyl Co A (top numbers) </li></ul>www.freelivedoctor.com
  20. 20. Beta Oxidation on 16 C fatty Acid totals <ul><li>7 rounds of beta oxidation </li></ul><ul><ul><li>7 rounds X FADH 2 X 1.5 ATP = 10.5 ATP </li></ul></ul><ul><ul><li> round FADH 2 </li></ul></ul><ul><ul><li>7 rounds X NADH X 2.5 ATP = 17.5 ATP </li></ul></ul><ul><ul><li> round NADH </li></ul></ul><ul><ul><li>8 acetyl CoA </li></ul></ul><ul><ul><li>8 Krebs </li></ul></ul><ul><ul><li>8 Krebs X 3 NADH X 2.5 ATP = 60 ATP </li></ul></ul><ul><ul><li> Krebs NADH </li></ul></ul><ul><ul><li>8 Krebs X FADH 2 X 1.5 ATP = 12 ATP </li></ul></ul><ul><ul><li> Krebs FADH 2 </li></ul></ul><ul><ul><li> 8 Krebs X GTP X 1 ATP = 8 ATP </li></ul></ul><ul><ul><li>Krebs GTP </li></ul></ul><ul><ul><li>1 ATP used to activate = - 1 ATP </li></ul></ul><ul><ul><li>Total 107 ATP </li></ul></ul>www.freelivedoctor.com
  21. 21. Unsaturated Fat Metabolism <ul><li>special enzymes needed to convert cis bonds in fatty acids to trans bonds </li></ul>www.freelivedoctor.com
  22. 22. Regulation of Fat Metabolism <ul><li>Transport is rate-limiting </li></ul><ul><li>Regulation of carnitine acyl transferase </li></ul><ul><ul><li>off by fat synth products </li></ul></ul><ul><ul><li>high NADH </li></ul></ul>www.freelivedoctor.com
  23. 23. Ketone Bodies <ul><li>Starvation causes accumulation of acetyl CoA </li></ul><ul><ul><li>not enough carbohydrates to keep Kreb’s Cycle going </li></ul></ul><ul><ul><li>acetyl CoA forms acetoacetate, b-hydroxybutyrate, and acetone. </li></ul></ul>www.freelivedoctor.com
  24. 24. Diabetes and Ketone Bodies <ul><li>When there is not enough insulin in the blood and it must break down fat for its energy. </li></ul><ul><li>Ketones build up in the blood and then spill over into the urine so that the body can get rid of them. Acetone can be exhaled through the lungs. This gives the breath a fruity odor. Ketones that build up in the body for a long time lead to serious illness and coma. (Diabetic ketoacidosis) </li></ul>www.freelivedoctor.com
  25. 25. Comparison of Fat Metabolism per gram and CHO per gram <ul><li>Fats provide about 9 kilocalories per gram and carbohydrates provide about 4 kilocalories per gram. </li></ul><ul><li>Using nutritional units, that is 9 Calories/gram for fats and 4 Calories/gram for carbohydrates. </li></ul><ul><li>CHO provide energy more quickly </li></ul><ul><li>Fats are good fuel for endurance events, but not sprints </li></ul>www.freelivedoctor.com

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