Cell Anatomy

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  • 7 Cell shape Positioning of organelles Intracellular “railroad track” Link to EC and other cells Movement
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  • 5 Tay-Sachs disease is a fatal genetic disorder in which harmful quantities of a fatty substance called ganglioside GM2 accumulate in the nerve cells in the brain. Infants with Tay-Sachs disease appear to develop normally for the first few months of life. Then, as nerve cells become distended with fatty material, a relentless deterioration of mental and physical abilities occurs. The child becomes blind, deaf, and unable to swallow. Muscles begin to atrophy and paralysis sets in. A much rarer form of the disorder which occurs in patients in their twenties and early thirties is characterized by unsteadiness of gait and progressive neurological deterioration. Patients with Tay-Sachs have a "cherry-red" spot in the back of their eyes. The condition is caused by insufficient activity of an enzyme called hexosaminidase A that catalyzes the biodegradation of acidic fatty materials known as gangliosides. Gangliosides are made and biodegraded rapidly in early life as the brain develops. Patients and carriers of Tay-Sachs disease can be identified by a simple blood test that measures hexosaminidase A activity. Both parents must be carriers in order to have an affected child. When both parents are found to carry a genetic mutation in hexosaminidase A, there is a 25 percent chance with each pregnancy that the child will be affected with Tay-Sachs disease. Prenatal monitoring of pregnancies is available if desired.
  • The Luxol fast blue stain highlights the large swollen neurons in Tay-Sachs disease.
  • 6 Peroxisomes seem to be to be self-replicating. They don’t come off the Golgi apparatus as secretory vesicles as originally thought.
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  • CAM = cell adhesion molecule Cadherins are Calcium dependant cell adhesion molecules. They play an important role in embryonic morphogenesis and the formation of solid tissues.
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  • Cell Anatomy

    1. 1. Background Basics: Units of measure Hydrophobic/philic molecules Proteins Compound molecules pH DNA and RNA CELL ANATOMY www.freelivedoctor.com
    2. 2. Key Concepts <ul><li>Cell anatomy </li></ul><ul><li>Tissue types </li></ul><ul><li>Tissue remodeling </li></ul><ul><li>Organs </li></ul>www.freelivedoctor.com
    3. 3. Compartments <ul><li>Major Body Cavities (thorax, etc.) </li></ul><ul><li>Fluid Compartments </li></ul><ul><ul><li>Intracellular Fluid (ICF) or cytosol </li></ul></ul><ul><ul><li>Extracellular fluid ECF </li></ul></ul><ul><ul><ul><li>Between Cells </li></ul></ul></ul><ul><ul><ul><li>Circulatory System (plasma) </li></ul></ul></ul><ul><li>Intracellular compartments </li></ul><ul><ul><li>Membranous organelles </li></ul></ul>www.freelivedoctor.com
    4. 4. Biological Membranes <ul><li>Two definitions: </li></ul><ul><ul><li>Body’s borders, e.g., </li></ul></ul><ul><ul><ul><li>Peritoneal membrane </li></ul></ul></ul><ul><ul><ul><li>Skin </li></ul></ul></ul><ul><ul><li>Cell membrane </li></ul></ul><ul><ul><ul><li>Phospholipid bilayer </li></ul></ul></ul><ul><ul><ul><li>Proteins and cholesterol interspersed </li></ul></ul></ul>www.freelivedoctor.com
    5. 5. Cell differentiation <ul><li>Mechanism: differential gene activation allows creation of specialized cells </li></ul>From 1 zygote to 200 different types of cells
    6. 6. Special Structural Features of Cell Membranes <ul><li>Microvilli </li></ul><ul><li>Cilia </li></ul><ul><li>Stereocilia </li></ul><ul><li>Flagella </li></ul>Function?
    7. 7. Cytoplasm <ul><li>Cytosol: </li></ul><ul><li>= semigelatinous intracellular fluid </li></ul><ul><li>Medium for suspension of </li></ul><ul><ul><li>Organelles, </li></ul></ul><ul><ul><li>Ions, nutrients, wastes, enzymes etc.……. </li></ul></ul><ul><ul><li>Inclusions </li></ul></ul><ul><li>Organelles perform </li></ul><ul><li>specialized tasks. </li></ul>Membranous organelles Non-membranous organelles
    8. 8. Cytoskeleton <ul><li>Strength </li></ul><ul><li>Support </li></ul><ul><li>Shape </li></ul><ul><li>Transport </li></ul><ul><li>Cell to cell links </li></ul><ul><li>Protein fibers </li></ul><ul><ul><li>Microfilaments </li></ul></ul><ul><ul><li>Intermediate </li></ul></ul><ul><ul><li>Microtubules </li></ul></ul>
    9. 9. Centrosomes and Centrioles <ul><li>1 centrosome contains 2 centrioles </li></ul><ul><li>Centrosomes organize microtubules </li></ul><ul><li>Centrioles: bundles of microtubules </li></ul><ul><ul><li>Pull chromosomes, form core in cilia </li></ul></ul>
    10. 10. <ul><li>Contain motor proteins </li></ul><ul><li>2:9 microtubule pattern </li></ul><ul><li>Cilia move fluids </li></ul><ul><li>Flagella move sperm cells </li></ul>Cilia and Flagella
    11. 11. <ul><li>Function: Transfer of messages from DNA </li></ul><ul><li>Fixed to ER or free in cytoplasm </li></ul>
    12. 12. Membranous Organelles <ul><li>Special compartments for special functions </li></ul><ul><ul><li>Separate harmful substances from other cell areas </li></ul></ul><ul><ul><li>Separate function from other cell areas </li></ul></ul>Mitochondrion = powerhouse of cell. Energy (ATP) production Has own DNA, self-replicating
    13. 13. RER & SER RER: Protein synthesis, strage, modification & transport vesicles SER: Synthesis and conversion of FA, steroids, lipids In muscle: Ca 2+ storage
    14. 14. Golgi Apparatus <ul><li>Modification (labeling) of proteins </li></ul><ul><li>Packaging into secretory (to ECF) or storage vesicles </li></ul>“ Post office” of cell
    15. 15. Protein Secretion
    16. 16. Lysosomes <ul><li>Digestion (~ 50 </li></ul><ul><li>enzymes) of </li></ul><ul><li>bacteria and old </li></ul><ul><li>organelles </li></ul><ul><li>Enzymes only </li></ul><ul><li>active at pH of </li></ul><ul><li>100 – 1,000 x </li></ul><ul><li>< cytoplasm </li></ul><ul><li>  pH = ? </li></ul>Also used to dissolve Ca-carbonate of bone and for self destruction of damaged cells Disorders such as rheumatoid arthritis and Tay-Sachs disease
    17. 17. Tay Sachs disease
    18. 18. Peroxi- somes <ul><li>Major function: Degradation of long chain FAs </li></ul><ul><li>Generate hydrogen peroxide  contain catalase </li></ul>Smaller than Lysosomes - Different set of enzymes
    19. 19. Nucleus <ul><li>Control Center </li></ul><ul><li>Nuclear envelope with nuclear pore complexes for diffusion and active transport </li></ul><ul><li>Chromatin (DNA and proteins) </li></ul><ul><li>DNA forms genes </li></ul><ul><li>One or more nucleoli </li></ul>
    20. 20. Cell to Cell Junctions <ul><li>Utilize CAMs (Cell Adhering Molecules) </li></ul><ul><ul><li>Tight Junctions </li></ul></ul><ul><ul><li>Anchoring Junctions </li></ul></ul><ul><ul><ul><li>Desmosomes </li></ul></ul></ul><ul><ul><li>Gap Junctions </li></ul></ul>
    21. 21. Gap Junctions <ul><li>Cylindrical proteins form channels </li></ul><ul><li>Can open and close </li></ul><ul><li>Electrical synapses </li></ul><ul><li>Rapid transfer of signals in cardiac & smooth muscle </li></ul>
    22. 22. Tight junctions <ul><li>Complete barrier (brick wall) </li></ul><ul><li>Fusion of adjacent cell membranes via claudin and occludin </li></ul><ul><li>Found in </li></ul><ul><ul><li>BBB </li></ul></ul><ul><ul><li>GI tract, kidneys </li></ul></ul>Tight vs. leaky epithelium
    23. 23. Movement of substances across tight and leaky epithelia
    24. 24. Anchoring Junctions <ul><li>Anchoring junctions (CAMs: cadherins) </li></ul><ul><ul><li>Desmosomes </li></ul></ul><ul><ul><li>Adherens junctions </li></ul></ul><ul><li>Cell matrix attachments (CAMs: integrins) </li></ul><ul><ul><li>Hemidesmosomes </li></ul></ul><ul><ul><li>Spot desmosomes or focal adhesions </li></ul></ul><ul><li>Cell to cell or cell to CT matrix </li></ul>
    25. 25. Histology <ul><li>Structure and function of all four basic tissue types : remember from Anatomy or review on your own (starting p. 72 with epithelia) </li></ul><ul><li>Definition of organ ? Example: skin (see p 83) </li></ul>
    26. 26. The Four Tissue Types <ul><li>Epithelia </li></ul><ul><ul><li>Protection, exchange, etc. </li></ul></ul><ul><li>Connective </li></ul><ul><ul><li>Extracellular Matrix (ground substance) </li></ul></ul><ul><ul><li>Includes adipose, blood, lymph </li></ul></ul><ul><li>Muscle </li></ul><ul><ul><li>Smooth, cardiac, skeletal </li></ul></ul><ul><li>Neural </li></ul><ul><ul><li>Neurons and neuroglia </li></ul></ul>
    27. 27. Stem Cells <ul><li>Review concept of stem cells </li></ul><ul><ul><li>Totipotent – earliest cells in zygote </li></ul></ul><ul><ul><li>Pluripotent – starting specialization </li></ul></ul><ul><ul><li>Multipotent – more specialized (bone marrow) </li></ul></ul><ul><li>Research: </li></ul><ul><ul><li>Fetal stem cells </li></ul></ul><ul><ul><li>Plasticity of adult stem cells </li></ul></ul>
    28. 28. Tissue Remodeling <ul><li>Tissue remodeling throughout a person’s life </li></ul><ul><li>Apoptosis = Programmed cell death (suicide) </li></ul><ul><ul><li>Cell breaks up into membrane bound blebs which will be phagocytosed by other cells. </li></ul></ul><ul><li>Necrosis = traumatic cell death </li></ul><ul><ul><li>Lack of O 2 , trauma, toxins </li></ul></ul><ul><ul><li>Cells rupture  tissue damage & inflammation </li></ul></ul>
    29. 29. Running Problem: The Pap Smear
    30. 30. Cervical cells. Uniform in size and shape  normal

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