Gene transcription is coordinated with, but not
dependent on, cell divisions during C. elegans
embryonic fate specification...
RNA-FISH!
10µm!
RNA!
Automated
Lineaging!
Gut genes!
Wild type
20C!
Transcription
is slowed
LESS?
Transcription
is slowed
MORE?
Transcription
is slowed
THE SAME?
Transcription
is slowed
THE SAME
Transcription
is slowed
THE SAME
- many other genes
- clk-1 mutant slowdown
Transcription
is slowed
THE SAME
- many other genes
- clk-1 mutant slowdown
Transcription
is slowed
THE SAME
Total Number of Cells in Embryo
Total Number of Cells in Embryo
Total Number of Cells in Embryo
Transcription is
DEPENDENT
on cell divisions?
emb-29(g52)
mutant
Total Number of Cells in Embryo
Transcription is
DEPENDENT
on cell divisions?
NO
Transcription is
DEPENDENT
on cell divisions?
NO
- elt-2 in E lineage
- hlh-1 and
unc-120
in muscle
pathway
Transcription is
DEPENDENT
on cell divisions?
NO
- elt-2 in E lineage
- hlh-1 and
unc-120
in muscle
pathway
Transcription is
DEPENDENT
on cell divisions?
NO
- elt-2 in E lineage
- hlh-1 and
unc-120
in muscle
pathway
Gene transcription is coordinated with, but not
dependent on, cell divisions during C. elegans
embryonic fate specification!
Gene transcription is coordinated with, but not
dependent on, cell divisions during C. elegans
embryonic fate specification...
Worm timing paper
Worm timing paper
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Worm timing paper

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Worm timing paper

  1. 1. Gene transcription is coordinated with, but not dependent on, cell divisions during C. elegans embryonic fate specification! Gautham  Nair,  Travis  Walton,  John  Isaac  Murray,  and  Arjun  Raj   University  of  Pennsylvania  Department  of  Bioengineering   University  of  Pennsylvania  Department  of  GeneDcs  
  2. 2. RNA-FISH! 10µm! RNA!
  3. 3. Automated Lineaging!
  4. 4. Gut genes! Wild type 20C!
  5. 5. Transcription is slowed LESS?
  6. 6. Transcription is slowed MORE?
  7. 7. Transcription is slowed THE SAME?
  8. 8. Transcription is slowed THE SAME
  9. 9. Transcription is slowed THE SAME - many other genes - clk-1 mutant slowdown
  10. 10. Transcription is slowed THE SAME - many other genes - clk-1 mutant slowdown
  11. 11. Transcription is slowed THE SAME Total Number of Cells in Embryo
  12. 12. Total Number of Cells in Embryo
  13. 13. Total Number of Cells in Embryo Transcription is DEPENDENT on cell divisions?
  14. 14. emb-29(g52) mutant Total Number of Cells in Embryo Transcription is DEPENDENT on cell divisions? NO
  15. 15. Transcription is DEPENDENT on cell divisions? NO - elt-2 in E lineage - hlh-1 and unc-120 in muscle pathway
  16. 16. Transcription is DEPENDENT on cell divisions? NO - elt-2 in E lineage - hlh-1 and unc-120 in muscle pathway
  17. 17. Transcription is DEPENDENT on cell divisions? NO - elt-2 in E lineage - hlh-1 and unc-120 in muscle pathway
  18. 18. Gene transcription is coordinated with, but not dependent on, cell divisions during C. elegans embryonic fate specification!
  19. 19. Gene transcription is coordinated with, but not dependent on, cell divisions during C. elegans embryonic fate specification! Funding:     HHMI  Life  Sciences  Research  FoundaDon  Fellowship   Burroughs-­‐Wellcome  Fund   NIH  New  Innovator  Award   Penn  Genome  FronDers  InsDtute   Pennsylvania  Department  of  Health  

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