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Presentation Group 3


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  • 1. Assessment of EnterobacteriaceaeFound
    in Juices Sold at Cagayan National
    Highschooland Cagayan State
    University-Andrews Campus
  • 2. CHAPTER I
    Background of the Study
    Significance of the Study
    Scope and Delimitation
  • 3. Records of PHO and CVMC show that there has been an increase in occurrence of Enterobacteriaceae-induced diseases.
    One factor that may be associated to the increase in number of cases is the continuous increase in the number of stalls and stores with inadequate sanitation
    The researchers will examine the presence of Enterobacteriaceae in juice drinks, rather than in solid foods
    A. Background of the Study
  • 4. Juice drinks will be taken from stalls and stores located within and nearby CSU-AC and CNHS
    If proven that Enterobacteriaceae exists in the juices, the researchers will determine if factors (other than hand washing) such as source of water, environmental condition, etc. may be the reason for the presence of the said organism in the juice drink
    It is therefore the researchers aim to prove that the growing number of stores and stalls is indeed associated to the increased cases of Enterobacteriaceae-induced diseases
    A. Background of the Study
  • 5. B. Objectives
    General Objective: The study aims to determine the presence of Enterobacteria in juices sold at Cagayan National Highschool and Cagayan State University-Andrews Campus.
    Identify the different species of Enterobacteria such as E.coli, S. typhosa and S. dysenteriae
    present in juices sold by stores and stalls at Cagayan National Highschool and Cagayan State University-Andrews Campus.
    Specific Objectives:
  • 6. 2. Compare the occurrence of the different
    Enterobacteria species.
    B. Objectives
    3. Determine the factors associated to the
    occurrence of the different Enterobacteria
    4. Determine the extent of the vendor’s
    knowledge, practices, attitude towards
    proper sanitation and food handling
  • 7. Department of Health
    Juice Vendors
    Students of CSU-AC and CNHS
    Other consumers
    C. Significance of the Study
  • 8. The Researchers
    Future Student Researchers
    C. Significance of the Study
  • 9. Enterobactericeae species:
    Salmonella typhosa
    Escherichia coli
    Stalls and stores within the vicinity of 30 meter radius from CNHS and CSU-AC
    Samples to be examined will be juice drinks that are manually prepared by the vendor regardless of its flavor, type (whether powdered or natural fruit juice) and source of water
    D. Scope and Delimitation
  • 10. From a total of 30 stalls and stores, only 23 will be chosen from which samples will be taken and the vendors of each will be used as respondents to the questionnaires that will be administered.
    Three replications will be carried out
    McConkey Agar will be the culture media used
    IMVIC Reaction will be the biochemical test applied
    D. Scope and Delimitation
  • 11. A. Paradigm
    B. Conceptual Framework
  • 12. A. The Paradigm
    Independent Variable
    Dependent Variable
    a. Juices sold by stores and stalls
    b. Water source
    c. Sanitation
    d. Vendor’s hygiene
    e. Food handling
    f. Environmental condition
    Growth of Enterobactriaceae in McConkey Agar
  • 13. The presence of bacteria in food and water that every individual consumes could bring about undesirable effects such as diseases. Therefore, correct hand washing and proper food handling should be observed to eliminate the occurrence of these food borne diseases. Since most of food handlers or vendors are not well-educated regarding proper sanitation and proper hygiene, it is expected that their knowledge, practices and attitude contribute greatly to the transmission of Enterobacteria.
    B. Conceptual Framework
  • 14. The paradigm shows both dependent and independent variables of which the factors such as juices, water source, sanitation, vendor’s hygiene, food handling, and environmental condition could bring about the growth of Enterobacteria in the culture media. Moreover, once proven that Enterobacteria are indeed present in any of the juices, it is assumed that consumers of such juices are at great risk of acquiring Enterobacteria-induced diseases; and it is also assumed that the environmental sanitation, and orientation of the vendor with regards to proper sanitation is unsatisfactory.
    B. Conceptual Framework
  • 15. Research Design
    Locale of the Study
    Research Instrumentation
    Respondents and Sampling Method
    Collection and Processing of Data
    Statistical Tools For Data Analysis
  • 16. Descriptive-evaluative
    A. Research Design
  • 17. This study will be conducted at Cagayan State University-Andrews Campus at the clinical laboratory of the College of Medical Technology
    B. Locale of the Study
  • 18. For bacterial culture
    For IMVIC Reaction
    Spot Indole Test
    Methyl Red Test
    Voges-Proskauer Test
    Citrate Test
    C. Research Instrumentation
  • 19. For Respondents: Non-probability Sampling Design
    Purposive Sampling Method
    For Juice Samples: Probability Sampling Design
    Simple Randomization Method
    D. Respondents and Sampling Method
  • 20. Slovin’sFormula
    n=N/[1+N(e) 2]
    D. Respondents and Sampling Method
  • 21. For the first three chapters:
    Letter to:
    Provicial Health Office (PHO)
    Cagayan Valley Medical Center (CVMC)
    Counting of stores and stalls
    Slovin’s Formula
    E. Collection and Processing of Data
  • 22. For the Succeeding Chapters
    Labeling of the stores and stalls with the use of numbers
    Purchasing of juice drinks as samples
    Testing of Samples
    Measuring of sample
    Biochemical test
    Motility testing (Salmonella typhosa and Shigelladysenteriae)
    E. Collection and Processing of Data
  • 23. Analysis of Variance
    Descriptive Statistics
    F. Statistical Tools
  • 24. THE END