Indonesian Crab Development Center
  CBAD TAKALAR - SOUTH SULAWESI
APLICATION OF PROBIOTIC AND MOLASES FOR
IMPROVING OF REARING MEDIA ON CRABLET
  MASS PRODUCTION OF SWIMMING CRAB
         ...
Introduction
• CBAD Takalar has been producing crablet of
  Portunus pelagicus since 2005
• Crablet production was unstabl...
Objective
• To know the effectiveness of probiotic
  bacteria and molases toward increasing the
  survival rate of swimmin...
Materials and methods

• Probiotic : Commercial product ”Develop TM”
• Molases: Sugarcan factory in Takalar – South
  Sula...
Materials and Methods
•   Conical fiber pond : 250 L
•   Stocking density : 60 ind/mL
•   Rearing period : 15 days
•   Die...
Materials and Methods
• Treatments :
1. Probiotic : 10 ppm
2. Probiotic with molases : 10 ppm of probiotic
   and 100 ppm ...
Data Collecting and Analysis
•   Growth stage of larvae
•   Survival rate
•   Population of bacteria
•   Water quality
Results & Discussion
               Table 1. Growth stage development of swimming crab larvae for 15 days rearing periode
...
100                                                            Figure 1.




Population of Growth stage (%) Population of ...
0.0025
                                           A                                                 Figure 2.
            ...
•Addition of molases into water rearing media of swimming crab tends
to reduce the growth stage, as larvae growth was opti...
100
                                                                                            Survival rate of probiotic...
Table 2. Population density of bacteria total and Vibrio sp. on each growth stage (cfu/ml).

                         Trea...
Table 3. Water quality performance in both treatments probiotic and probiotic with molases of
                         rea...
Conclusion & Recommendation
          Application of the probiotic bacteria with molases in water media of swimming
crab l...
Further Information
CENTER FOR BRACKISHWATER AQUACULTURE DEVELOPMENT
                      TAKALAR
                     AD...
Application Of Probiotic And Molases
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Application Of Probiotic And Molases

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Application Of Probiotic And Molases

  1. 1. Indonesian Crab Development Center CBAD TAKALAR - SOUTH SULAWESI
  2. 2. APLICATION OF PROBIOTIC AND MOLASES FOR IMPROVING OF REARING MEDIA ON CRABLET MASS PRODUCTION OF SWIMMING CRAB (Portunus pelagicus, Linn) Sugeng Raharjo Nana S.S. Udi Putra Eddy Nurcahyono Makulau Sultan
  3. 3. Introduction • CBAD Takalar has been producing crablet of Portunus pelagicus since 2005 • Crablet production was unstable • Attacking of pathogen bacteria (Vibrio sp) caused a production loss • Environmant was become an issue global-food safety and human health • Preventive treatment: probiotic added for reduction pathogen bacteria but its has a short life time, so need the substrute for feeding • Probiotic plays as a biological balance for bacteria pathogen like Vibrio sp.
  4. 4. Objective • To know the effectiveness of probiotic bacteria and molases toward increasing the survival rate of swimming crab larvae
  5. 5. Materials and methods • Probiotic : Commercial product ”Develop TM” • Molases: Sugarcan factory in Takalar – South Sulawesi Indonesia
  6. 6. Materials and Methods • Conical fiber pond : 250 L • Stocking density : 60 ind/mL • Rearing period : 15 days • Diet : zoeae-1 to zoeae-4 : Branchionus plicatilis (density 10-15 ind/ml) and fatty acid Ω3– HUFA (100 ppm) and multivitamin (100 ppm) zoeae-3 until finish : Artemia salina (4 times per day)
  7. 7. Materials and Methods • Treatments : 1. Probiotic : 10 ppm 2. Probiotic with molases : 10 ppm of probiotic and 100 ppm of molases • added every 3 day after water exchange
  8. 8. Data Collecting and Analysis • Growth stage of larvae • Survival rate • Population of bacteria • Water quality
  9. 9. Results & Discussion Table 1. Growth stage development of swimming crab larvae for 15 days rearing periode TREATMENTS REARING PERIOD ROBIOTIC PROBIOTIC AND MOLASES (day) 1 Zoeae-1 Zoeae-1 GROWTH STAGE 2 Zoeae-1 Zoeae-1 3 Zoeae-1 Zoeae-1(10%)/ Zoeae-2 (90%) 4 Zoeae-2 Zoeae-2 5 Zoeae-3 Zoeae-2 6 Zoeae-3 Zoeae-3 7 Zoeae-3 Zoeae-4 8 Zoeae-4 Zoeae-4 9 Zoeae-4 (40%)/Megalop-1 (60%) Zoeae-4 10 Zoeae-4(90%)/ Megalop-1 (10%) Megalop-1 11 Megalop-1 Megalop-2 12 Megalop-2 Megalop-3 13 Megalop-4 Megalop-3 14 Megalop-5 (80%)/Crablet-1(20%) Megalop-4 15 Crablet-1 Megalop-5/Crablet-1 (10%)
  10. 10. 100 Figure 1. Population of Growth stage (%) Population of Growth stage (%) 90 20% Growth stage development of 80 70 60% swimming crab larvae (A) 60 90% Treatment of Probiotic and Population of growth stage (%) 50 molases and (B) Treatment of 40 A probiotic only. 30 20 10 0 100 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Rearing of the day 20% 10% 90 Addition of molases into Zoeae-1 Zoeae-2 Zoeae-3 Zoeae-4 Megalops Crablet 80 70 water rearing media of 60 swimming crab was 50 accelerated growth stage. 40 B Its mean that larvaes have a 30 high growth performance 20 10 than using probiotic only. 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Rearing period Zoeae-1 Zoeae-2 Zoeae-3 Zoeae-4 Megalops Crablet
  11. 11. 0.0025 A Figure 2. Probiotic 0.0020 Growth of swimming carb Probiotic and Molases larvae in rearing period (A) 0.0015 body weight and (B) lenght of Weight (g) carapace 0.0010 0.0005 0.0000 Body weight and Lenght of 1.80 1 3 5 7 9 11 13 15 carapace of probiotic and 1.60 B Probiotic molases treatment were more 1.40 lenght and weight than Lenght of carapace (mm) Probiotic and 1.20 Molases treatment probiotic only but 1.00 not significantly different 0.80 (P>0,05). 0.60 Have a tending that probiotic 0.40 and molases treatment was 0.20 0.00 higher performance growth 1 3 5 7 9 11 13 15 Rearing period
  12. 12. •Addition of molases into water rearing media of swimming crab tends to reduce the growth stage, as larvae growth was optimal. •In the final rearing period showed lenght of carapace and weight body of treatment probiotic and molases were more lenght and weight than treatment of probiotic only but not significantly different (P>0,05). But unsignificant different between both treatment might be caused by molases concentration and C-N ratio of feed were not optimal yet
  13. 13. 100 Survival rate of probiotic and Probiotic molases treatment were higher 80 Probiotic and Molases survival rate than treatment SURVIVAL RATE probiotic only but not Survival rate (%) 60 significantly different (P>0,05) for Z2 and Crablet stage. 40 Thereby Probiotic and molases played an important role in 20 increasing SR. 0 Z1 Z2 Z3 Z4 M C Growth stage Figure 3. Survival rate of each stage on rearing period of swimming crab larvae
  14. 14. Table 2. Population density of bacteria total and Vibrio sp. on each growth stage (cfu/ml). Treatments Parameters Growth Stage Zoeae-1 Zoeae-2 Zoeae-3 Zoeae-4 Megalop POPULATION OF BACTERIA Probiotic Bacteria Total 2,4 X 103 2,0 X 104 3,2 X 104 1,1 X 103 6,0 X 104 Vibrio sp 2,0 X 102 2,0 X 103 1,2 X 103 2,1 X 102 4,2 X 103 Probiotic and Bacteria Total 2,0 X 104 3,4 X 104 4,6 X 104 4,2 X 106 4,2 X 106 Molases Vibrio sp 3,2 X 103 3 X 102 3,2 X 102 2,2 X 102 2 X 102 Treatment of probiotic and molases was higher population density of bacteria total than treatment of probiotic only. Its indicated that molases plays an important role in providing nutrition for probiotic. In the both treatments showed that the presence of population of probiotic bacteria will be able to inhibit the population of bactera pathogen. Aplication probiotic in the water media will have benefit more if probiotic could growth and developed well, furthermore nutrition should be provided. So in this research addition of molases as substrate and nutrition also important.
  15. 15. Table 3. Water quality performance in both treatments probiotic and probiotic with molases of rearing of swimming crab larvae (Portunus pelagicus,linn) Parameters Treatment Probiotic Probiotic dan Molases WATER QUALITY Dissolved Oxygen (mg/l) 4,8 – 5,6 4,6 – 6,2 pH 7,8 – 8,6 8,1 – 8,4 Temperature (0C) 27 – 31 27 – 31 Salinity (ppt) 33 – 35 33 -35 Ammonia (mg/l) Not detected Not detected Nitrit (mg/l) Not detected Not detected
  16. 16. Conclusion & Recommendation Application of the probiotic bacteria with molases in water media of swimming crab larvae (Portunus pelagicus,linn) tends to increase the survival rate, body weight, lenght of carapace, and able to accelerate the growth of zoeae stage, in addition also controlled the population growth of Vibrio sp (102 cfu/ml). Furthermore using probiotic with molases was effective in improving of rearing water media on mass producing crablet of swimming crab. For the further it should be conducted the detail study in using of concentration molases, probiotic density and other combination substrate for getting the high survival rate and growth such as C-N ratio.
  17. 17. Further Information CENTER FOR BRACKISHWATER AQUACULTURE DEVELOPMENT TAKALAR ADDRESS : BONTOLOE VILLAGE SOUTH GALESONG –TAKALAR 92245 SOUTH SULAWESI – INDONESIA TEL : 62-411-320730 FAX: 62-418-2327666
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