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Introducing: Green Fluorescent Nanoparticle-labeled human Mesenchymal Stem Cells  These cells add to our fluorescein, rhod...
FITC analysis of cells during second passage following transfection. Image obtaining using FITCfilter on Olympus CKX41 mic...
FITC analysis of cells during third passage following transfection. Image obtaining using FITCfilter on Olympus CKX41 micr...
FITC analysis of cells during fourth passage following transfection. Image obtaining using FITCfilter on Olympus CKX41 mic...
Osteogenic differentiation of Grn-fluorescent nanoparticle-labeled MSCs. Image obtaining using phasecontrast Olympus CKX41...
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Human mesenchymal stem cells green fluorescent nanoparticle-labeled sc00-a4np technical information

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These cells are generated by chemical transfection of CdSe/ZnS nanoparticles, 100 nm in diameter and show a strong fluorescent signal (Excitation maximum= 515 nm; Emission maximum= 520-550 nm) detectable by standard FITC filters. These cell retain GFNP labeling through 3+ passages and can be differentiation into chondrogenic, adipogenic or osteogenic lineages. Excellent for regeneration, toxicity and related studies.


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Transcript of "Human mesenchymal stem cells green fluorescent nanoparticle-labeled sc00-a4np technical information"

  1. 1. Introducing: Green Fluorescent Nanoparticle-labeled human Mesenchymal Stem Cells These cells add to our fluorescein, rhodamine and GFP-expressing MSCs and offer an alternative fluorescent MSCs with the following properties: •Generated by chemical transfection of CdSe/ZnS nanoparticles, 100 nm in diameter •Strong fluorescent signal (Exmax= 515 nm; Emissionmax= 520-550 nm) detectable by standard FITC filters. •Retains fluorescence through growth or differentiation into chondrogenic, adipogenic or osteogenic lineages. •Possible indicator of endoplasmic processing within MSCs •Fluorescence is detectable in three successive passages and diminishes with passage. •Useful in tracking and imaging investigations based on MSCs
  2. 2. FITC analysis of cells during second passage following transfection. Image obtaining using FITCfilter on Olympus CKX41 microscope at 200 X equipped with a Retiga 2000 digital camera andQ-Capture software program. Scale bar is 25 micrometers.
  3. 3. FITC analysis of cells during third passage following transfection. Image obtaining using FITCfilter on Olympus CKX41 microscope at 200 X equipped with a Retiga 2000 digital camera and Q-Capture software program. Scale bar is 25 micrometers. Note that this culture is at higher celldensity than the previous image.
  4. 4. FITC analysis of cells during fourth passage following transfection. Image obtaining using FITCfilter on Olympus CKX41 microscope at 200 X equipped with a Retiga 2000 digital camera and Q-Capture software program. Scale bar is 25 micrometers. Fluorescent nanoparticles are lessprevalent within the cytoplasm than earlier passages.
  5. 5. Osteogenic differentiation of Grn-fluorescent nanoparticle-labeled MSCs. Image obtaining using phasecontrast Olympus CKX41 microscope at 100 X equipped with a Retiga 2000 digital camera and Q-CapturePro software program. Scale bar is 25 micrometers. Note the presence of mineral deposits indicatingdifferentiation into osteoblasts. Chondrogenic and adipogenic differentiation was also apparent .
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