Artificial insemination

3,512 views
3,296 views

Published on

For more exciting veterinary stuff visit http://www.techyvety.com

0 Comments
3 Likes
Statistics
Notes
  • Be the first to comment

No Downloads
Views
Total views
3,512
On SlideShare
0
From Embeds
0
Number of Embeds
4
Actions
Shares
0
Downloads
379
Comments
0
Likes
3
Embeds 0
No embeds

No notes for slide

Artificial insemination

  1. 1. Artificial Insemination Heather Borgia Cal Poly San Luis Obispo , CA Compilation: AECD, California || For more Veterinary stuff visit http://www.techyvety.com
  2. 2. <ul><ul><ul><ul><li>Arab Chieftains - Stole semen to breed mares </li></ul></ul></ul></ul><ul><ul><ul><ul><li>Ivanov (Russia) 1900 - </li></ul></ul></ul></ul><ul><ul><ul><ul><ul><li>- Developed methods as we know today </li></ul></ul></ul></ul></ul><ul><ul><ul><ul><ul><li>- Most work was with horses but did some cattle and </li></ul></ul></ul></ul></ul><ul><ul><ul><ul><ul><li>pig work </li></ul></ul></ul></ul></ul><ul><ul><ul><ul><li>First US AI Cooperative - 1937 </li></ul></ul></ul></ul><ul><ul><ul><ul><li>Dairy cooperatives merge and form large companies that dominate cattle AI industry - 1960’s to present </li></ul></ul></ul></ul><ul><ul><ul><ul><li>All turkey’s bred AI - 1960’s to present </li></ul></ul></ul></ul><ul><ul><ul><ul><li>Expansion of swine and horse AI - 1990’s </li></ul></ul></ul></ul>Breif History Compilation: AECD, California || For more Veterinary stuff visit http://www.techyvety.com
  3. 3. <ul><ul><ul><ul><li>Genetic improvement of livestock </li></ul></ul></ul></ul><ul><ul><ul><ul><li>Disease control mechanism </li></ul></ul></ul></ul><ul><ul><ul><ul><li>Possible to increase fertility </li></ul></ul></ul></ul><ul><ul><ul><ul><li>Decrease breeding expense </li></ul></ul></ul></ul>Objectives of Artificial Insemination Compilation: AECD, California || For more Veterinary stuff visit http://www.techyvety.com
  4. 4. Current Status of US Industry Dairy Cattle - 7 million (50%) Beef Cattle - 1.3 million (3%) Swine - 20 - 30% Turkey - 100% Horses - Increasing rapidly Who uses Artificial Insemination? Compilation: AECD, California || For more Veterinary stuff visit http://www.techyvety.com
  5. 5. <ul><li>Genetic Improvement </li></ul><ul><li>Wide spread availability of genetically superior sires </li></ul><ul><li>Disease Control </li></ul><ul><li>Injured studs available </li></ul><ul><li>More valid stud proofs </li></ul><ul><li>Reduced danger from studs </li></ul><ul><li>Cost - relatively cheap </li></ul>Advantages Compilation: AECD, California || For more Veterinary stuff visit http://www.techyvety.com
  6. 6. <ul><ul><ul><ul><li>Estrus detection must be good </li></ul></ul></ul></ul><ul><ul><ul><ul><li>Handle and care for semen </li></ul></ul></ul></ul><ul><ul><ul><ul><li>Record Keeping </li></ul></ul></ul></ul><ul><ul><ul><ul><li>Time involved - Restraining and inseminating cow </li></ul></ul></ul></ul><ul><ul><ul><ul><li>Training required to handle semen and breed cow </li></ul></ul></ul></ul>Disadvantages Compilation: AECD, California || For more Veterinary stuff visit http://www.techyvety.com
  7. 7. <ul><li>Semen Collection </li></ul><ul><li>Sexual arousal using Sight, sound, smell, touch </li></ul><ul><li>Best mount - Live </li></ul><ul><li>Alternative mount - dummies available for: </li></ul><ul><li>Stallions </li></ul><ul><li>Boars </li></ul><ul><li>Bulls </li></ul>Where do you start? Compilation: AECD, California || For more Veterinary stuff visit http://www.techyvety.com
  8. 8. <ul><li>Electro-ejaculation </li></ul><ul><ul><ul><ul><li>Useful if male won’t or can’t mount </li></ul></ul></ul></ul><ul><ul><ul><ul><li>Get urine often </li></ul></ul></ul></ul><ul><ul><ul><ul><li>Poorer quality ejaculate </li></ul></ul></ul></ul><ul><ul><ul><ul><li>Used in rams and beef bulls </li></ul></ul></ul></ul><ul><li>Massage Method </li></ul><ul><ul><ul><ul><li>Stimulate by rectal massage </li></ul></ul></ul></ul><ul><ul><ul><ul><li>Seminal vesicles </li></ul></ul></ul></ul><ul><ul><ul><ul><li>Vas deferens </li></ul></ul></ul></ul>Methods of Collecting Semen Compilation: AECD, California || For more Veterinary stuff visit http://www.techyvety.com
  9. 9. <ul><li>Artificial Vagina </li></ul><ul><ul><ul><li>Temperature </li></ul></ul></ul><ul><ul><ul><li>Bull, stallion, ram at 45°C </li></ul></ul></ul><ul><ul><ul><li>Pressure </li></ul></ul></ul><ul><ul><ul><li>Friction </li></ul></ul></ul><ul><ul><ul><li>Lubrication essential </li></ul></ul></ul>Compilation: AECD, California || For more Veterinary stuff visit http://www.techyvety.com
  10. 10. Extenders include: Nutrients Glucose, fructose Cold shock prevention Buffer Milk, skim-milk, egg yolk Citrate, Tris The buffer component Antibiotics Inhibit bacterial growth Increase volume Cryoprotectant glycerol Preservation of Semen Extend then Freeze! Compilation: AECD, California || For more Veterinary stuff visit http://www.techyvety.com
  11. 11. <ul><li>After collected and extended, semen is put into a semen straw. </li></ul><ul><li>That straw is placed into a Liquid Nitrogen Tank. </li></ul><ul><li>Each tank contains a Cane, which consists of Goblets containing the semen straws themselves. </li></ul><ul><li>Make sure that the </li></ul><ul><li>correct procedure for </li></ul><ul><li>freezing semen in a liquid </li></ul><ul><li>nitrogen is followed. </li></ul>Storage of Semen Compilation: AECD, California || For more Veterinary stuff visit http://www.techyvety.com
  12. 12. <ul><ul><ul><ul><li>Use the procedures recommended by the semen supplier!!! </li></ul></ul></ul></ul><ul><ul><ul><ul><li>35°C water for 30 - 60 seconds </li></ul></ul></ul></ul><ul><ul><ul><ul><li>Ice water for 3 minutes </li></ul></ul></ul></ul><ul><ul><ul><ul><li>Pocket thaw </li></ul></ul></ul></ul><ul><ul><ul><ul><li>Be very careful when removing straw from Nitrogen Tank </li></ul></ul></ul></ul>Thawing Compilation: AECD, California || For more Veterinary stuff visit http://www.techyvety.com
  13. 13. <ul><li>Time of insemination </li></ul><ul><ul><ul><ul><ul><li>Cow - 12 hours after observed in standing heat (AM - PM rule) </li></ul></ul></ul></ul></ul><ul><ul><ul><ul><ul><li>Sow - 24 and 36 hours after first seen in estrus </li></ul></ul></ul></ul></ul><ul><ul><ul><ul><ul><li>Gilt - 12 and 24 hours after first seen in estrus </li></ul></ul></ul></ul></ul><ul><ul><ul><ul><ul><li>Sheep -12 to 18 hours after first seen in estrus </li></ul></ul></ul></ul></ul><ul><ul><ul><ul><ul><li>Horses -Every second day beginning on day 3 of heat </li></ul></ul></ul></ul></ul><ul><li>Insemination Protocol </li></ul><ul><li>Rectal/ Vaginal </li></ul><ul><li>Vaginal </li></ul>Once you have collected the semen and detected estrus of the female, you can begin to inseminate. Detection of Estrus Compilation: AECD, California || For more Veterinary stuff visit http://www.techyvety.com
  14. 14. <ul><li>Pipette with syringe </li></ul><ul><li>Lube </li></ul><ul><li>Semen Straw </li></ul><ul><li>Gloves (Arms Length) </li></ul>Things you Need Compilation: AECD, California || For more Veterinary stuff visit http://www.techyvety.com
  15. 15. <ul><li>Begin by restraining the animal. This will protect the animal and the technician during the procedure. </li></ul>STEP 1: Procedure Compilation: AECD, California || For more Veterinary stuff visit http://www.techyvety.com
  16. 16. <ul><li>The inseminator places their arm into the females rectum, using plenty of lubrication, and removes all feces. This will allow the inseminator to palpate for the cervix more easily. </li></ul>STEP 2: Be sure to use plenty of lubrication. You Can Never Use To Much Lube!!!!!!!! Compilation: AECD, California || For more Veterinary stuff visit http://www.techyvety.com
  17. 17. <ul><li>Once the inseminator has located the cervix they will hold it in their hand. </li></ul><ul><li>Using their free hand the inseminator will obtain the syringe with a plastic sheath (pipette) already prepared with a straw of semen. </li></ul>STEP 3 & 4: Compilation: AECD, California || For more Veterinary stuff visit http://www.techyvety.com
  18. 18. Carefully inserting the syringe with semen, being sure not to contaminate it with feces or other contaminates, the inseminator will insert the syringe into the females vagina. They will continue to pass the syringe until they can pass it through to the end of the cervix. STEP 5: Compilation: AECD, California || For more Veterinary stuff visit http://www.techyvety.com
  19. 19. Once the end of the cervix is reached, the plunger on the syringe can be depressed allowing the semen to be deposited directly into the uterus of the female. The Sperm will then hopefully reach and fertilize the egg resulting in conception, and eventually a fetus. STEP 6: Compilation: AECD, California || For more Veterinary stuff visit http://www.techyvety.com
  20. 20. The procedure in sows is slightly different from that used in Cows and Mares. There is no rectal palpation necessary in sows, only passing a pipette into the vagina. Once passed, a bottle of extended semen is placed at the end of the pipette. No pressure is needed on the bottle, the sows reproductive track will complete the procedure. The bottle of extended semen does need to be protected from the light and temperature changes to protect the sperm. What about Pigs? Compilation: AECD, California || For more Veterinary stuff visit http://www.techyvety.com
  21. 21. <ul><li>Factors Effecting Conception Rate </li></ul><ul><ul><ul><ul><li>Time of insemination </li></ul></ul></ul></ul><ul><ul><ul><ul><li># of sperm inseminated </li></ul></ul></ul></ul><ul><ul><ul><ul><li>Fertility of males </li></ul></ul></ul></ul><ul><ul><ul><ul><li>Skill of inseminator </li></ul></ul></ul></ul>Does it work every time? Compilation: AECD, California || For more Veterinary stuff visit http://www.techyvety.com
  22. 22. Success If all goes well, the female animal becomes pregnant and carries the baby to term. Then once the baby is born it will be the result of a successful Artificial Insemination procedure. Compilation: AECD, California || For more Veterinary stuff visit http://www.techyvety.com

×