Microscope wall temperature inside enclosure compared: Halogen lamp Vs Fiber-light
Why fiber-light is important ?• DNA experiments are really sensitive to temperature (thermalenergy) even few degrees make a big difference.• Microscope (optical trap) is enclosed inside an enclosure (to avoidairborne noise and currents), so within few hours temperature of themicroscope wall rises almost twice due to the halogen lamp used forillumination, this heats up the entire enclosure so as the sample.• Temperature hike may make two similar data sets which are takensome time apart different.• Fiber light solves the problem of temperature hike by providing cleanlight and by removing heat production through the lamp andconduction through the microscope skin.• With fiber light temperature inside the enclosure goes from 23.7 to25.5 total 1.8 degree Celsius hike in comparison to halogen lampwhere temperature goes from 23.7 to 49 total 25.3 degree hike over aperiod of 10 hours (see slide 3).
Construction of Fiber-light system Fiber light has 4 parts Olympus power supply Model TH4-100 Dolan Jenner optical fiber bundle Fiber-light feeder Illuminator & fiber plugin adapter
Olympus Power supply Model TH4-100 TH4-100 has 4 holes Connect +ve 12V and ground with a wire at one end and other end is banana connectors
Construction of fiber plugin adapter at illuminator Illuminator Fiber plugin adapter
Construction Steps Remove illuminator from microscopeRemove the Halogen lamp Remove all the enclosures
Illuminator with fiber plugin adapter is complete
Cushion Z- adjustment ScrewsPut a rubber cushion (optional) and insert the fiber through the opening and tight the screws
Construction of Fiber-feeder Lamp mount Lens assembly Fiber outlet adapter 6 X3X2.5 inches aluminum box
Banana connectors Prepare the box 3/8’’ hole for 3/8’’ holes for rubber legs lamp mount 1.25’’ 2.25’’ .5’’ .5’’ 50mm .25’’ Lamp side Bottom side ¼’’ hole for lens assembly 3/8’’ hole for fiber outlet adapter 1’’ hole for fiber outlet Fiber outlet side
Preparing Lamp Mount PCB platesBanana connectors Metal plates Halogen lamp 4/40’’ blind hole ½’’ long in PCB WARNING: Lamp gets really HOT so DON’T use plastic, wood or anything which burns below 500o Celsius
3/8’’ through holes• I used 6 fold of PCB plates to mount the metal plates with ½’’ long blind screws.• Metal plates are aluminum plates which I machined to fit the lamp wire connectors.• Metal plates are attached into PCB plates with 4/40’’ blind screws.• PCB plates are screwed to the wallwith 3/8’’ screws.• PCB plate can tolerate hightemperatures and providegood isolation for the metal plates. 4/40’’ screw on 3/8’’ holes in metal
Insert adapter into coupler and then coupler into cage plate• On wood side cage plate do not use the wood like shown in the picture.• Put another cage plate on that sidewith another adapter and coupler.• I did not have extra set in my lab so I used wood.
Mount in with 3/8’’ screw to the boxDo not use wood use 2 cage plates with 2 couplers and 2 adapters
Preparing Lens assembly CP02T-thorlabs cage platesLA1951-A thorlabs f25mm lensRA90-thorlabs post clamp ER2-thorlsba 3’’ cage assembly rodTR2-thorlabs post Lens is to focus the light on the fiber inlet
Put lens assembly on the box Slide in z-direction to get optimum spot on the fiber inlet 50mmxy-adjustment to center the spot (lamp image) on the fiber inlet Lens assembly gives 3-D adjustment to the lens
Prepare the cover • Halogen lamp heats up the box • I did not put any fan inside because fan generates vibrations which can travel through the fiber bundle to the microscope and make optical trap noisy. • So fan has to be outside with no physical contact with the box. • To accommodate that I made 2’’ diameter holes. • Fan will be mounted from top trough the roof.