Buffers used in dna overstretching and unzipping experiments

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We primarily use popping buffer and PBS for DNA-unzipping and overstretching experiments. All the other buffers consists these two.

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Buffers used in dna overstretching and unzipping experiments

  1. 1. Buffers used in DNA-overstretching and unzipping experiments
  2. 2. We primarily use popping buffer and PBS for DNA-unzipping and overstretching experiments • Popping Buffer • PBS
  3. 3. Popping BufferChemicals used in popping buffer:• EDTA:
  4. 4. • Sodium Phosphate• Monobasic:
  5. 5. • Dibasic
  6. 6. • NaCl
  7. 7. • Tween20:
  8. 8. • Water H2O:
  9. 9. • D2O
  10. 10. Quantities and measurements
  11. 11. Calculations LabView
  12. 12. Weight & Measure
  13. 13. Store
  14. 14. PBSChemicals used in PBS: PBS and H2O or D2O • Dissolve 1 table in 200mL of H2O or D2O
  15. 15. BGBBlotting Grade Blocker To make 5mg/ml BGB solution in popping buffer: •First wiight out 15mg of BGB poweder •Add 1.5 ml of 1x pop and mix by vortexing •Run through .2μM syringe filter using 3ml syringe. •Now store 5mg/ml BGB in 1x pop buffer (minus amount of protein that stuck to filter) at 3C. This should be good for few weeks.
  16. 16. Filter
  17. 17. Anti-Dig
  18. 18. Microspheres
  19. 19. DNA

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