Work better seen than said!
Upcoming SlideShare
Loading in...5
×
 

Work better seen than said!

on

  • 846 views

 

Statistics

Views

Total Views
846
Views on SlideShare
846
Embed Views
0

Actions

Likes
0
Downloads
0
Comments
0

0 Embeds 0

No embeds

Accessibility

Upload Details

Uploaded via as Microsoft Word

Usage Rights

© All Rights Reserved

Report content

Flagged as inappropriate Flag as inappropriate
Flag as inappropriate

Select your reason for flagging this presentation as inappropriate.

Cancel
  • Full Name Full Name Comment goes here.
    Are you sure you want to
    Your message goes here
    Processing…
Post Comment
Edit your comment

Work better seen than said! Work better seen than said! Document Transcript

  • A Pictorial representation of what I am currently doing:MTT assays.Fig 1: Orange color due to addition of MTT reagent to detect cell viability in eachwellFig 2: Initial plating and treatment of mammalian cells in 10% FBS media
  • FACS analysis performed with 6 well plates.Cells per well : 175KMedia: 3 mL in each well2-Day to 3- Day treatments depending on the purposeROS determining agent added on Day 2 of the treatment, prepared fresh.Also done to determine Annexin-PI binding of cells showing usual apoptosis(e.g Annexin binds to the phospholipids, facing outside, of a programmed to diecell and thus this activity can determine apoptosis/necrosis of the treated cells)
  • DRUG PREPARATIONS (High school Chemistry ) DRUGS kept on ice before use DRUG samples (including stocks) in Storage FRESH drug dilutions prepared for treatment