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Entomological field techniques for mosquito and sandfly
 

Entomological field techniques for mosquito and sandfly

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This is all about the entomological field techniques for the collection of mosquito and sandfly.

This is all about the entomological field techniques for the collection of mosquito and sandfly.

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    Entomological field techniques for mosquito and sandfly Entomological field techniques for mosquito and sandfly Presentation Transcript

    • ENTOMOLOGICAL FIELD TECHNIQUES FOR MOSQUITO AND SAND FLY PRAKASH DHAKAL Public Health Microbiology Tribhuvan University, Nepal
    • OBJECTIVES  To operate different equipments in the field for collecting mosquito and sand fly .
    • INTRODUCTION  Entomological field techniques are generally used in vector-borne diseases.  Vector borne diseases are Malaria, JE,Filariasis,Dengue , Kala-azar etc.  Based on local conditions prevalent; optimum combination of these techniques should be used.  Entomological investigations are important and essential aspect of vector control.  These investigations provide information on vector species , their distribution, density, bionomics and susceptibility/resistance to insecticides for vector control.
    •  These investigations are also useful for the monitoring of potential vectors and their role in disease transmission.  Information collected from entomological surveillance techniques helps in understanding the spatial and temporal changes in vector species, efficacy and effectiveness of vector control measures.
    • SAMPLING REQUIREMENTS  Vehicle to get around.  Good topographical map or at least a plat or city map.  Data sheets.  Boots.  Dipper, collecting jars, small type net.  Water proof marking pens, tape etc.  Carrying box or container, ice chest etc.  Data logger, GPS
    • SAMPLING EQUIPMENTS  Dipper  Pipette  Aspirator  Flashlight Aspirator  Larval Container  Bag for extra vials, pens, labeling tape, etc.
    • METHODOLOGY  For BG-Sentinel trap.  BG- Sentinel trap is generally used for the collection of Aedes type of mosquito.
    •  First of all the trap is held in upright position by three stands in three sides.  Then the electric plug is connected.  Then mosquito attracting chemical is placed in the pocket inside the trap.  Then the white colored net is covered from the top.  After that a black colored collection box along with two successive nets are inserted through the white colored net.  After complete assemblage it is connected with electric source, generally of 12 volt.
    • WORKING MECHANISM OF BG SENTINEL TRAP  Aedes mosqutio are attracted by the chemical which is placed inside the trap.  The odor of chemical is like the smell of socks which is worn for several days.  Then aedes mosquito gets attached in the black colored collection box .  After attachment the mosquito gets inside the collection box due to the negative pressure created by the fan operating inside the trap.  Then mosquito gets collected inside the net.
    •  For CDC Miniature Light Trap
    •  Developed by CDC for portable collection of mosquitoes and sand flies.  Standard survey tool for mosquito collections.  Operates on 6 volts.
    • WORKING MECHANISM  After completely assembling the equipment it is connected with the electric source of 6 volt.  The main mechanism for collection of mosquito is attraction due the light emitted by the bulb of this instrument.  This attraction is called Phototrophism.
    • ANIMAL BAITED NET TRAP
    •  In animal baited technique a net of 5 x 5 x 5 m is taken.  From the ground about 1 feet of space is left for the entry of mosquito in the net.  The net is made such that the mosquito entering the net do not comes out.
    •  Mouth Aspirator
    •  From mouth aspirator resting mosquito is collected.  This aspirator is kept near the resting mosquito and is sucked .  After collecting in the aspirator, mosquito is kept in the paper cup covered with net.
    •  Ovi trap
    •  For ovi trap, about 10-15 ml of clean water is taken in a black colored paper cup and in the edge of that cup a sky blue cloth is kept. After few days eggs of mosquito are seen in the cloth.
    • WINDOW TRAP COLLECTION (EXIT TRAPS)
    •  Window trap collection is done for those mosquitoes which bites indoor and goes outside for the rest.
    • HUMAN LANDING CATCHES  In this type of collection technique , humans are directed to keep up their pants or trousers up to the knee and the shirt up to their shoulder. Mosquito is allowed to bite to human . immediately at the instant the biting mosquito is sucked through the mouth aspirator .  Since biting of mosquito may spread the disease if it is carrying organism responsible for disease , so nowadays it is not used.
    •  Sticky paper is placed on the land when sand fly jumps over it , it gets stick on the paper. STICKY TRAP: SANDFLY
    • CONCLUSION  Different instruments and techniques were used for the collection of mosquito and sandfly .
    • FIELD PHOTOS AND VIDEOS :
    •  Sincere thanks to respected Lecturer Dr. Ishan Gautam, Natural History Museum , IOST, TU for showing the breeding sites of vector ( Mosquito) and teaching the techniques of collection ,processing and many others…………….
    • THANK YOU