0
Principle, Reagents & MediaProcedures & End resultsBIOCHEMICAL ACTIVITIES
BIOCHEMICAL ACTIVITIES OF BACTERIA:Biochemical properties could be used as tools  of identification  demonstrate certain ...
IMVIC - INDOLE TEST Principle : product of hydrolysis of an amino acid  tryptophan Media – SIM (Sulfide Indole Motility)...
IMVIC - METHYL RED TEST  Methyl   red is a pH indicator  Principle : production of sufficient acidity from   glucose  M...
IMVIC - VOGUES PROSKAUER• production of acetyl-methyl carbinolfrom glucose•media: MR-VP broth•reagent: 40% KOH•Positive (+...
IMVIC - CITRATE UTILIZATION TEST     Principle: utilizes citrate as a source of Carbon   Media   :Simmon Citrate (SCA)  ...
OTHER COMMON BIOCHEMICAL TESTS: Coagulase test Catalase test Urease test Hemolysis test Greig’s Test Oxidase Test H...
ASSIGNMENT:   Find the other biochemical Tests & tabulate    them based on:     Working principle     Reagents & Media ...
WHAT TO DO? Place all your glassware in a plastic bag Autoclave 15 psi, 121 C, 15 minutes Wash , clean and dry Wrap an...
MEDIA PREPARATION   Prepare 200 ml each ( for the whole class)     SIM     MRVP     SCA   Dispense into 4 test tubes ...
 Inoculate with bacteria ( get from me ) Incubate in incubator 48 hours Add the reagents ( from previous slides ) Chec...
Module 5b biochemical activities for the lab
Upcoming SlideShare
Loading in...5
×

Module 5b biochemical activities for the lab

1,666

Published on

dr lu5b

Published in: Education, Business, Technology
0 Comments
0 Likes
Statistics
Notes
  • Be the first to comment

  • Be the first to like this

No Downloads
Views
Total Views
1,666
On Slideshare
0
From Embeds
0
Number of Embeds
0
Actions
Shares
0
Downloads
24
Comments
0
Likes
0
Embeds 0
No embeds

No notes for slide

Transcript of "Module 5b biochemical activities for the lab"

  1. 1. Principle, Reagents & MediaProcedures & End resultsBIOCHEMICAL ACTIVITIES
  2. 2. BIOCHEMICAL ACTIVITIES OF BACTERIA:Biochemical properties could be used as tools of identification  demonstrate certain metabolic activity of microbesExample : Fermentation – durham tubes Carbohydrates, amino acids, organic acids  1. gases – Carbone dioxide, methane, hydrogen  2. Acid production – use of indicators to detect  Either acid or gas or both can be collected
  3. 3. IMVIC - INDOLE TEST Principle : product of hydrolysis of an amino acid tryptophan Media – SIM (Sulfide Indole Motility) Reagent - (Ehrlich’s) Para-dimethyl- aminobenzaldehyde Positive result :(+) pink or red color Negative Result : (-) absence of pink or red color
  4. 4. IMVIC - METHYL RED TEST  Methyl red is a pH indicator  Principle : production of sufficient acidity from glucose  Media- MR-VP broth  (+) red - acid  (-) yellow - alkaline
  5. 5. IMVIC - VOGUES PROSKAUER• production of acetyl-methyl carbinolfrom glucose•media: MR-VP broth•reagent: 40% KOH•Positive (+) result = production ofan eosin like color
  6. 6. IMVIC - CITRATE UTILIZATION TEST  Principle: utilizes citrate as a source of Carbon  Media :Simmon Citrate (SCA)  (+) blue color  (--) green color
  7. 7. OTHER COMMON BIOCHEMICAL TESTS: Coagulase test Catalase test Urease test Hemolysis test Greig’s Test Oxidase Test Hydrogen Sulfide Test
  8. 8. ASSIGNMENT: Find the other biochemical Tests & tabulate them based on:  Working principle  Reagents & Media  Positive & Negative Results
  9. 9. WHAT TO DO? Place all your glassware in a plastic bag Autoclave 15 psi, 121 C, 15 minutes Wash , clean and dry Wrap and sterilize in the oven, 150 C, 45 minutes
  10. 10. MEDIA PREPARATION Prepare 200 ml each ( for the whole class)  SIM  MRVP  SCA Dispense into 4 test tubes 1 tube SIM  2 tubes MRVP  1 tube SCA ( Slant )
  11. 11.  Inoculate with bacteria ( get from me ) Incubate in incubator 48 hours Add the reagents ( from previous slides ) Check results Make a one page write – up Submit
  1. A particular slide catching your eye?

    Clipping is a handy way to collect important slides you want to go back to later.

×