Module 5a biochemical activities

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Module 5a biochemical activities

  1. 1. Biochemical Tests Principles Reagents & MediaProcedures & Results
  2. 2. IMViCx Indole - x Methyl Red - – product of hydrolysis – pH indicator of tryptophan – production of – Reagent - (Ehrlichs) sufficient acidity Para-dimethyl- from glucose aminobenzaldehyde – Media- MR-VP – (+) pink or red color broth – (+) red - acid – (-) yellow - alkaline
  3. 3. IMViC (2)x Vogues Proskauer - x Citrate Utilization Test – production of – citrate as a source acetyl-methyl of Carbon carbinol from – media :Simmon glucose Citrate (SCA) – media: MR-VP – (+) blue color broth – (-) green color – reagent: 40% KOH – (+) production of an eosin like color
  4. 4. Biochemical Tests (3)x Urease Test - x Oxidase Test - – Hydrolysis of urea – catalysis of Oxidase by Proteus sp. through cytochrome – media: by N. gonorrhea Christensen’s urea – media: CAP – (+) Pink color media – reagent: 1% para- /hemolysis amino-dimethyl- – (-) colorless aniline hydrochloride – (+) black
  5. 5. Biochemical Tests (4)x Catalase Test - x Coagulase Test - – decomposition of – coagulation of 3% Hydrogen plasma with enzyme peroxide coagulase by – (+) rapid evolution pathogenic of gas staphylococcus – organisms: – media: Blood plasma y(+) staphylococci – (+) clotting of plasma y micrococci y (-) streptococci
  6. 6. Biochemical Tests (5)x Hemolysis test - x Hydrogen Sulfide – destruction of RBC test - – media : Blood agar – production of plate Hydrogen sulfide – (+) clearing zone – any media withx Greig’s Test Fe – hemolysis of – (+) black ppt. sheep’s rbc x Pigment formation – (+) El tor (-) vibrio – chromogens
  7. 7. Enzymes produced by bacteria:x Constitutive - essential enzymesx Adaptive - non-essential enzymesx Intracellularx Extracellular

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