The Immunobiology of Oil-in-Water Adjuvants for Influenza Vaccines
My objective...This will be done in the context of the PCIRN RT-03 StudySubjects were given the Canadian pandemic H1N1 influenza vaccine...And essentially, our hypothesis is that...
STUDY RESULTSEpub Dec 2010The RT-03 was completed between November 2009 and January 2010(Pediatric) study showed that one pediatric dose of adjuvanted pH1N1 vaccine elicited potentially protective titers (≥ 40) in 80% of seronegative children while a second dose raised this to 100% and increased GMT by 10-fold. Naturally primed children had strong responses; such high titers may afford greater or longer-lasting protection.
Geometric mean with 95% confidence intervalof HAI, MN, Avidity ELISAs
1. The Immunobiology of Oil-in-Water Adjuvants for Influenza Vaccines<br />Karen K. Yam, Ph.D.<br />Post-doctoral Fellow (Brian Ward Lab)<br />PCIRN Annual Meeting<br />Wednesday April 20th, 2011<br />
2. Objectives<br />Understand the influence of adjuvants on long-term humoral responses to influenza following vaccination<br />PCIRN RT-03 Study<br />Canadian pH1N1 influenza vaccine<br />ASO3 adjuvant<br />25% of the antigen dose of regular vaccine<br />Hypothesis<br />Low dose of antigen in adjuvanted vaccine may enhance the short-term response at the expense of the long-term immunity<br />
5. Methods:Serum Analysis<br />Hemagglutination Inhibition (HAI) assay<br />Titer of flu-specific Abs<br />National Microbiology Laboratory<br />Micro-neutralization (MN) assay<br />Titer of functional Abs that can neutralize viral entry<br />Avidity Index ELISA<br />Measure of the binding strength of flu-specific Ab<br />Read-out for the development of humoralimmresp<br />
12. Primed AI- : 9/18 (50%)</li></li></ul><li>Conclusions/Questions<br />Why is there a failure to increase antibody avidity following a second immunization?<br />20/73 = 27% of all subjects<br />11/55 = 20% of naive subjects with AI<br /> 9/18 = 50% of primed subjects with no change<br />Maturation of the humoral immune response was abnormally truncated<br />Effect of low antigen dose in adjuvanted vaccine?<br />Role of pre-existing immunity on the response to influenza vaccines?<br />
13. Acknowledgements<br />Dr. Brian J. Ward<br />Dr. Jyotsana Gupta<br />Angela Brewer<br />Members of Ward lab<br /><ul><li>Dr. David Scheifele