Human Lactoferrin
Upcoming SlideShare
Loading in...5
×
 

Human Lactoferrin

on

  • 757 views

 

Statistics

Views

Total Views
757
Views on SlideShare
757
Embed Views
0

Actions

Likes
0
Downloads
3
Comments
0

0 Embeds 0

No embeds

Accessibility

Categories

Upload Details

Uploaded via as Microsoft PowerPoint

Usage Rights

© All Rights Reserved

Report content

Flagged as inappropriate Flag as inappropriate
Flag as inappropriate

Select your reason for flagging this presentation as inappropriate.

Cancel
  • Full Name Full Name Comment goes here.
    Are you sure you want to
    Your message goes here
    Processing…
Post Comment
Edit your comment

Human Lactoferrin Human Lactoferrin Presentation Transcript

  • Human Lactoferrin
    • Lactoferrin is a glycoprotein secreted by mucosal membranes; it is a major protein in the secondary granules of leukocytes
    • During intestinal inflammation, activated leukocytes infiltrate the mucosa and lumen, increasing the level of fecal lactoferrin
    • Fecal lactoferrin is elevated in patients suffering from active inflammatory bowel disease (IBD) but not in persons with irritable bowel syndrome (IBS) or healthy persons
    • 1200
    • 800
    • 400
    • 0
    Lactoferrin levels (ug/g wet weight) Crohn’s Disease N=78 Ulcerative Colitis N=71 Irritable Bowel Syndrome N=31 Healthy Persons N=56 Lactoferrin levels in disease
    • IBD-CHEK TM – a qualitative ELISA for the clinical lab
    • IBD-QUIK CHEK TM – a qualitative lateral flow test for the clinical lab or physician’s office
    Two new noninvasive tests to detect intestinal inflammation
  • IBD-CHEK TM Test
    • Qualitative ELISA for detecting elevated fecal lactoferrin as an indicator of intestinal inflammation
    • Utilizes a four-step procedure with results in 75 minutes
    • A valuable noninvasive diagnostic aid to distinguish active IBD from IBS
    • Available by direct order for hospital and reference labs
  • Performance characteristics of the IBD-CHEK TM Test N=236 Total IBD-CHEK TM IBD-CHEK TM Crohn’s disease Active 52 85% 15% Inactive 26 62% 39% Ulcerative colitis Active 40 88% 13% Inactive 31 52% 48% Active IBS 31 0% 100% Healthy persons 56 0% 100% (+) (-)
  • Clinical results with the IBD-CHEK TM
    • When distinguishing active IBD from irritable bowel syndrome and healthy persons, the IBD-CHEK TM test exhibited a sensitivity of 86% and specificity of 100%
    • When distinguishing active Crohn’s disease from irritable bowel syndrome and healthy persons, the IBD-CHEK TM test exhibited a sensitivity of 85% and specificity of 100%
  • IBD-QUIK CHEK TM Test
    • Lateral flow test for detecting elevated fecal lactoferrin as an indicator of intestinal inflammation
    • Helps to distinguish active IBD from IBS
    • Turnaround time in 10 minutes using a single specimen dilution
    • Suitable for use in the clinical lab or Physician’s office
  • Performance characteristics of the IBD-QUIK CHEK TM Test N=137 Total IBD-QUIK IBD-QUIK CHEK TM (+) CHEK TM (-) Crohn’s disease Active 58 100% 0% Inactive 35 6% 94% Ulcerative colitis Active 12 100% 0% Inactive 11 0% 100% Active IBS 17 0% 100% Healthy persons 27 0% 100%
  • Clinical results with the IBD-QUIK CHEK TM
    • When compared to the IBD-CHEK TM test for distinguishing active IBD from active irritable bowel syndrome and healthy persons, the IBD-QUIK CHEK TM test showed a sensitivity of 100% and specificity of 98%
    • When distinguishing active IBD from inactive IBD and active irritable bowel syndrome, the IBD-QUIK CHEK TM test showed a sensitivity of 100% and specificity of 96%
  • Fecal lactoferrin testing
    • The IBD-CHEK TM and IBD-QUIK CHEK TM tests are sensitive and specific in vitro diagnostic aids for distinguishing active IBD from IBS
    • Both assays offer a cost effective method for screening patients
    • IBS accounts for 2.4 to 3.4 million office visits annually; fecal lactoferrin testing can help distinguish this condition from more serious IBD
  • References
    • 1. Dwarakanath, A. D., I. A. Finnie, C. M. Beesley, G. M. O’Dowd, J. Nash, H. H. Tsai, N. Parker, and J. Rhodes. 1997. Differential excretion of leucocyte granule components in inflammatory bowel disease: implications for pathogenesis. Clinical Science. 92: 307-313.
    • Guerrant, R. L., V. Araujo, E. Soares, K. Kotloff, A. Lima, W. Cooper, and A. Lee. 1992. Measurement of fecal lactoferrin as a marker of fecal leukocytes. J. Clin. Microbiol. 30:1238-1242.
    • Kane, S., Sanborn, W. Rufo, P., Zholudev, A., Boone, J., Lyerly, D., Camilleri, M., and S. Hanauer. 2001. Fecal lactoferrin is a sensitive and specific marker for chronic inflammatory bowel disease. Abstract of Digestive Disease Week / American Gastroenterology Association, Atlanta, Georgia.
    • Kayazawa, M., O. Saitoh, K. Kojima, K. Nakagawa, S. Tanaka, K. Tabata, R. Maysuse, K. Uchida, M. Hoshimoto, I. Hirata, and K. Katsu. 2002. Lactoferrin in whole gut lavage fluid as a marker for disease activity in inflammatory bowel disease: Comparison with other neutrophil-derived proteins. Am. J. Gastroenterol. 97:360-369.
    • Fine, K.D., F. Ogunji, J. George, M. Niehaus, and R. Guerrant. 1998. Utility of a rapid fecal latex agglutination test detecting the neutrophil protein, lactoferrin, for diagnosing inflammatory causes of chronic diarrhea. Am. J. Gastroenterol. 93:1300-1305.
    • 6. Longstreth, G. F. , A. Wilson, K. Knight, J. Wong, C. Chiou, V. Barghout, F. Frech and J. Ofman. 2003. Irritable bowel syndrome, health care use, and costs: A U.S. managed care perspective. Am. J. Gastroenterol. 98:600-607.
    • 7. Riley, L. W. 1995. Acute inflammatory diarrhea. In M. Blaser (ed.), P. Smith, J. Ravdin, H. Greenberg, and R. Guerrant, Infections of the Gastrointestinal Tract . Raven Press, New York, NY.
    • 8. Sugi, K., Saithoh, O., Hirata, I., and K. Katsu. 1996. Fecal lactoferrin as a marker for disease activity in inflammatory bowel disease: Comparison with other neutrophil-derived proteins. Am. J. Gastroenterol. 91:927-934.
    • 9. Uchida, K., R. Matsuse, S. Tomita, K. Sugi, O. Saitoh, and S. Ohshiba. 1994. Immunochemical detection of human lactoferrin in feces as a new marker for inflammatory gastrointestinal disorders and colon cancer. Clin. Biochem. 27:259-264.