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Particularly, in feminine mice modeling human heart failure, the advantages of sildenafil ranged from robust to virtually nonexistent, depending on the animals' ranges of the hormone estrogen, says Takimoto, an assistant professor of drugs and a researcher with the Heart and Vascular Institute at Johns Hopkins. In male mice, sildenafil usually seems to work nicely, but solely as a result of it targets a unique biological course of independent of estrogen, he says. Estrogen is present in both female and male mammals, although in several quantities.