Author(s): Matthew Velkey, 2009License: Unless otherwise noted, this material is made available under the terms of theCrea...
Citation Key                          for more information see: http://open.umich.edu/wiki/CitationPolicyUse + Share + Ada...
Muscle Tissue              Matthew Velkey, Ph.D.Fall 2008
Muscle TissueI. Striated Muscle - regularly arranged contractile units    A. Skeletal Muscle - long, cylindrical multinucl...
Skeletal Muscle InvestmentsEpimysium -dense irr. c.t.Perimysium -less dense irr. c.t.Endomysium -basal lamina andreticular...
Skeletal Muscle as seen in longitudinal section in the light microscope...•    Multi-nucleated and striated•    A bands - ...
Skeletal Muscle as seen in transverse section in the light microscope...      Figure 2 from Plate 18. Ross and Pawlina   F...
Organization of                           Skeletal Muscle Fibers                           THE SARCOMERE…                 ...
Transverse Section of Skeletal Muscle: TEM view•  H zone: thick   filaments only    – At M-line: thick      filaments and ...
Longitudinal Section of Skeletal Muscle as observed in the TEMI Band - Actin onlyA Band - Actin and MyosinH Zone - Myosin ...
T-tubule System:Propagation of the Signal  and Release of Ca2+T (transverse) Tubules•  run perpendicular (transversely)   ...
Longitudinal section of muscle showing triads• 1 T tubule• 2 terminal cisternae of sarcoplasmic reticulum•  Normally at A/...
Neuromuscular Junction                          Synapse:                          •  Action potential (AP) stimulates     ...
Muscle Innervation:                       Motor End PlateSource Undetermined                            Dake, Wikipedia   ...
Ca2+ Stimulates Myosin-Actin Binding and Initiates Contraction     •    Myosin-actin binding inhibited by TnI     •    TnC...
Contraction Cycle                    2                                                               3      1             ...
Sliding Filament Theory                            Sarcomere                                                        Source...
Cardiac MuscleTissue Features:•  Striated (same contractile machinery)•  Self-excitatory and electrically coupled•  Rate o...
Cardiac Muscle (longitudinal section)Source Undetermined
Cardiac Muscle (longitudinal section)                             Cardiac Muscle (transverse section)        Gartner and H...
Transverse Section of Cardiac Muscle versus Skeletal Muscle           Ross and Pawlina Figure 3 from Plate 18 on right. Le...
Cardiac Muscle (TEM) T Tubule/SR Diads    Left from Junquiera. Figure 10-24.    Right: Source Undetermined
Intercalated Discs Couple Heart Muscle Mechanically and Electrically          Junquiera. Figure 10-25.
aka “Fascia adherens”Transverse portion:forms mechanical coupling                                                         ...
Smooth Muscle•  Fusiform, non-striated cells•  Single, centrally-placed nucleus•  Contraction is non-voluntary•  Contracti...
Smooth Muscle (longitudinal section)        Gartner and Hiatt. Color Atlas of Histology. Figure 1 from plate 6.6 on left. ...
Smooth Muscle Viewed in Transverse    and Longitudinal Section                                     Color Atlas by Gartner ...
Ultrastructure of Smooth Muscle:•    actin and myosin filaments•    intermediate filaments of desmin (also vimentin in vas...
Smooth                                                                                     Muscle                         ...
More Ultrastructure of Smooth Muscle Cells:•  microtubules (curved arrows) •  dense bodies (desmin/vimentin plaques)•  act...
Smooth Muscle Contraction:         also Ca+ dependent, but mechanism is different than striated muscle       1. Ca2+ ions ...
Mechanics of Smooth                       Muscle Contraction                    •  Dense bodies are                       ...
Smooth Muscle (vascular)Source Undetermined   Source Undetermined    Relaxed             Contracted
Smooth Muscle                                                      VERSUSColor Atlas of Histology. Figure 1 from plate 8.2...
Epithelium                             Slide 250 vaginaB.V.B.V.                                            CT             ...
10-100µm in                          diameter                          Up to 30cm in                          length      ...
Skeletal Muscle                              Cardiac Muscle   Smooth Muscle    Left: Ross and Pawlina. Figure 2 from plate...
Muscle Regeneration and GrowthSkeletal Muscle•  Increase in size (hypertrophy)•  Increase in number (regeneration/prolifer...
Skeletal Muscle Satellite Cell Source Undetermined
Activated satellite cell in skeletal muscle    Source Undetermined
Learning Objectives1. Be able to identify the three types of muscle at the light and   electron microscope levels, includi...
Additional Source Information                             for more information see: http://open.umich.edu/wiki/CitationPol...
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09.15.08: Muscle Tissue

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09.15.08: Muscle Tissue

  1. 1. Author(s): Matthew Velkey, 2009License: Unless otherwise noted, this material is made available under the terms of theCreative Commons Attribution – Non-Commercial – Share Alike 3.0 License:http://creativecommons.org/licenses/by-nc-sa/3.0/We have reviewed this material in accordance with U.S. Copyright Law and have tried to maximize your ability to use,share, and adapt it. The citation key on the following slide provides information about how you may share and adapt thismaterial.Copyright holders of content included in this material should contact open.michigan@umich.edu with any questions,corrections, or clarification regarding the use of content.For more information about how to cite these materials visit http://open.umich.edu/education/about/terms-of-use.Any medical information in this material is intended to inform and educate and is not a tool for self-diagnosis or areplacement for medical evaluation, advice, diagnosis or treatment by a healthcare professional. Please speak to yourphysician if you have questions about your medical condition.Viewer discretion is advised: Some medical content is graphic and may not be suitable for all viewers.
  2. 2. Citation Key for more information see: http://open.umich.edu/wiki/CitationPolicyUse + Share + Adapt { Content the copyright holder, author, or law permits you to use, share and adapt. } Public Domain – Government: Works that are produced by the U.S. Government. (USC 17 § 105) Public Domain – Expired: Works that are no longer protected due to an expired copyright term. Public Domain – Self Dedicated: Works that a copyright holder has dedicated to the public domain. Creative Commons – Zero Waiver Creative Commons – Attribution License Creative Commons – Attribution Share Alike License Creative Commons – Attribution Noncommercial License Creative Commons – Attribution Noncommercial Share Alike License GNU – Free Documentation LicenseMake Your Own Assessment { Content Open.Michigan believes can be used, shared, and adapted because it is ineligible for copyright. } Public Domain – Ineligible: Works that are ineligible for copyright protection in the U.S. (USC 17 § 102(b)) *laws in your jurisdiction may differ { Content Open.Michigan has used under a Fair Use determination. } Fair Use: Use of works that is determined to be Fair consistent with the U.S. Copyright Act. (USC 17 § 107) *laws in your jurisdiction may differ Our determination DOES NOT mean that all uses of this 3rd-party content are Fair Uses and we DO NOT guarantee that your use of the content is Fair. To use this content you should do your own independent analysis to determine whether or not your use will be Fair.
  3. 3. Muscle Tissue Matthew Velkey, Ph.D.Fall 2008
  4. 4. Muscle TissueI. Striated Muscle - regularly arranged contractile units A. Skeletal Muscle - long, cylindrical multinucleated cells with peripherally placed nuclei. Contraction is typically quick and vigorous and under voluntary control. Used for locomotion, mastication, and phonation. B. Cardiac Muscle - elongated, branched cells with a single centrally placed nucleus and intercalated discs at the ends. Contraction is involuntary, vigorous, and rhythmic.II. Smooth Muscle - possesses contractile machinery, but it isirregularly arranged (thus, non-striated). Cells are fusiform with acentral nucleus. Contraction is involuntary, slow, and long lasting.
  5. 5. Skeletal Muscle InvestmentsEpimysium -dense irr. c.t.Perimysium -less dense irr. c.t.Endomysium -basal lamina andreticular fibers National Cancer Institute, Wikipedia ALL MUSCLE CELLS HAVE BASAL LAMINAE!
  6. 6. Skeletal Muscle as seen in longitudinal section in the light microscope...•  Multi-nucleated and striated•  A bands - anisotropic (birefringent in polarized light)•  I bands - isotropic (do not alter polarized light)•  Z lines (Zwischenscheiben, Ger. “between the discs”)•  H zone (hell, Ger. “clear”) Gartner and Hiatt. Color Atlas of Histology. Figure 1.
  7. 7. Skeletal Muscle as seen in transverse section in the light microscope... Figure 2 from Plate 18. Ross and Pawlina Figure 3 from Plate 18. Ross and Pawlina
  8. 8. Organization of Skeletal Muscle Fibers THE SARCOMERE… Contractile unit of striated muscle •  Structures between Z lines •  2 halves of I bands •  A band •  H zone •  M line (Mittelscheibe, Ger. “middle of the disc”) •  Myofilaments •  Actin •  Myosin •  Other structural proteins •  Titin (myosin-associated) •  Nebulin (actin-associated)Sameerb, Wikipedia •  Myomesin (at M line) •  α actinin (at Z line) •  Desmin (Z line) •  Vimentin (Z line) •  Dystrophin (cell membrane)
  9. 9. Transverse Section of Skeletal Muscle: TEM view•  H zone: thick filaments only – At M-line: thick filaments and myomesin lattice•  A band: thick & thin filaments•  I Band: thin filaments onlyI H M A Figure 10-11 from Junquiera. Junquiera. Figure 10-12.
  10. 10. Longitudinal Section of Skeletal Muscle as observed in the TEMI Band - Actin onlyA Band - Actin and MyosinH Zone - Myosin onlyS.R./T-tubule “triad” () mitochondria Sarcoplasmic reticulum Sarc. ret. T-tubule Junquiera. Figure 10-16.
  11. 11. T-tubule System:Propagation of the Signal and Release of Ca2+T (transverse) Tubules•  run perpendicular (transversely) to myofibrils•  conduct membrane depolarization deep into fibers Image of T-tubule system removedSarcoplasmic Reticulum•  smooth ER•  site of Ca2+ storage & release•  terminal cisternae abut T-tubules forming triads when myofibrils are viewed in longitudinal section
  12. 12. Longitudinal section of muscle showing triads• 1 T tubule• 2 terminal cisternae of sarcoplasmic reticulum•  Normally at A/I junctions in mammals (this sample is from an amphibian) Junquiera. Figure 10-10.
  13. 13. Neuromuscular Junction Synapse: •  Action potential (AP) stimulates release of acetylcholine from axon terminal into synaptic cleft •  Acetylcholine in synaptic cleft binds Na+ channel receptors –initiates sarcolemma AP Signal Propagation: T (transverse) Tubules •  Run perpendicular (transversely) to myofibrils •  Conduct membrane depolarization deep into fibers Intracellular Ca2+ release: Sarcoplasmic Reticulum •  Smooth ER, site of Ca2+ storage •  Voltage-gated channels in SR detect membrane depolarization in T-tubule and release Ca2+Source Undetermined
  14. 14. Muscle Innervation: Motor End PlateSource Undetermined Dake, Wikipedia 1. presynaptic terminal 2. sarcolemma 3. synaptic vesicles 4. Acetylcholine receptors 5. mitchondrion
  15. 15. Ca2+ Stimulates Myosin-Actin Binding and Initiates Contraction •  Myosin-actin binding inhibited by TnI •  TnC binds Ca2+ (if present) and induces release of TnI from actin •  Myosin binds actin; hydrolysis of ATP induces power stroke •  Actin filaments move relative to myosin Top left from Ross and Pawlina. Figure 11.7. Bottom left and right. Figures 10-13 and 10-14 (right). Junquiera.
  16. 16. Contraction Cycle 2 3 1 4 Alberts. Molecular Biology of the Cell.1.  ATP binds myosin – myosin releases actin2.  ATP hydrolysis induces conformational change – myosin head cocks forward 5nm (ADP+Pi remain bound to myosin).3.  Myosin binds weakly to actin, causing release of Pi4.  Release of Pi induces strong binding, power stroke, and release of ADP Myosin remains bound to actin if no more ATP is available (rigor conformation)
  17. 17. Sliding Filament Theory Sarcomere Source Undetermined Muscle fibers are composed of many contractile units (sarcomeres) Changes in the amount of overlap between thick and thin filaments allows for contraction and relaxation of muscle fibers Many fibers contracting together result in gross movementNote: Z lines move closer together; I band andH band become smaller during contraction Source Undetermined
  18. 18. Cardiac MuscleTissue Features:•  Striated (same contractile machinery)•  Self-excitatory and electrically coupled•  Rate of contractions modulated by autonomic nervous system –  innervation is neuroendocrine in nature (i.e. no “motor end plates”)Cell Features:•  1 or 2 centrally placed nuclei•  Branched fibers with intercalated discs•  Numerous mitochondria (up to 40% of cell volume)•  Sarcoplasmic reticulum & T-tubules appear as diads at Z lines –  Sarcoplasmic reticulum does not form terminal cisternae –  T tubules are about 2x larger in diameter than in skeletal muscle •  transport Ca2+ into fibers
  19. 19. Cardiac Muscle (longitudinal section)Source Undetermined
  20. 20. Cardiac Muscle (longitudinal section) Cardiac Muscle (transverse section) Gartner and Hiatt. Color Atlas of Histology. Figure 2 and Figure 4 from Plate 6.8
  21. 21. Transverse Section of Cardiac Muscle versus Skeletal Muscle Ross and Pawlina Figure 3 from Plate 18 on right. Left U-M Histology Collection
  22. 22. Cardiac Muscle (TEM) T Tubule/SR Diads Left from Junquiera. Figure 10-24. Right: Source Undetermined
  23. 23. Intercalated Discs Couple Heart Muscle Mechanically and Electrically Junquiera. Figure 10-25.
  24. 24. aka “Fascia adherens”Transverse portion:forms mechanical coupling Junquiera. Figure 10-26 Source UndeterminedLateral Portion:forms electrical coupling Cross and Mercer. Cells and Tissue Ultrastructure. 1993. Plate 120
  25. 25. Smooth Muscle•  Fusiform, non-striated cells•  Single, centrally-placed nucleus•  Contraction is non-voluntary•  Contraction is modulated in a neuroendocrine manner•  Found in blood vessels, GI and urogenital organ walls, dermis of skin Junquiera. Figure 10-1.
  26. 26. Smooth Muscle (longitudinal section) Gartner and Hiatt. Color Atlas of Histology. Figure 1 from plate 6.6 on left. Figure 2 from plate 6.6.
  27. 27. Smooth Muscle Viewed in Transverse and Longitudinal Section Color Atlas by Gartner and Hiatt. Figure 4 from plate 6.6. Junquiera. Figure 10-30.
  28. 28. Ultrastructure of Smooth Muscle:•  actin and myosin filaments•  intermediate filaments of desmin (also vimentin in vascular smooth muscle)•  membrane associated and cytoplasmic dense bodies containing α actinin (similar to Z lines)•  relatively active nucleus (smooth muscle cells make collagen, elastin, and proteoglycans) May, Naftel, and Ard. University of Mississippi Digital EM Atlas. Plate 17A.
  29. 29. Smooth Muscle Viewed in Cross Section (TEM) What is the structure marked by * ? Also, note collagen – SMC secrete ECM: * collagen (I,III, IV), elastin, and proteoglycansMay, Naftel, and Ard. University of Mississippi Digital EM Atlas. Plate 17B
  30. 30. More Ultrastructure of Smooth Muscle Cells:•  microtubules (curved arrows) •  dense bodies (desmin/vimentin plaques)•  actin filament (arrowheads) •  caveoli (membrane invaginations & vesicular•  intermediate filaments system contiguous with SER –functionally analogous to sarcoplasmic reticulum) Cross and Mercer. Inset of plate 114.
  31. 31. Smooth Muscle Contraction: also Ca+ dependent, but mechanism is different than striated muscle 1. Ca2+ ions released from caveloae/SER and complex with calmodulin 2. Ca2+-calmodulin activates myosin light chain kinase 3. MLCK phosphorylates myosin light chain 4. Myosin unfolds & binds actin; ATP-dependent contraction cycle ensues. 5. Contraction continues as long as myosin is phosphorylated. 6. “Latch” state: myosin head attached to actin dephosphorylated causing decrease in ATPase activity –myosin head unable to detach from actin (similar to “rigor mortis” in skeletal muscle).Triggered by:•  Voltage-gated Ca+ channels activated by depolarization •  Mechanical stimuli •  Neural stimulation•  Ligand-gated Ca+ channels Ross and Pawlina. Figure 11.23.
  32. 32. Mechanics of Smooth Muscle Contraction •  Dense bodies are analogous to Z lines (plaques into which actin filaments insert) •  Myosin heads oriented in “side polar” arrangement •  Contraction pulls denseImage of smooth bodies together muscle cell contraction removed Additional notes: •  Contraction cycle generally about ~10% as fast as skeletal muscle •  Visceral (unitary) smooth muscle cells may be electrically coupled via gap junctions and exhibit either rhythmic or tonic contraction –innervation generally MODIFIES smooth muscle activity rather than initiating it. •  Multiunit smooth muscle cells are innervated individually and can contract rapidly for more precise control. •  Innervation is always at a distance (no motor end plates)
  33. 33. Smooth Muscle (vascular)Source Undetermined Source Undetermined Relaxed Contracted
  34. 34. Smooth Muscle VERSUSColor Atlas of Histology. Figure 1 from plate 8.2. Nerve Color Atlas of Histology. Figure 3 from plate 6.6 VERSUS Connective TissueColor Atlas of Histology. Figure 1 from plate 7.5 Color Atlas of Histology. Figure 3 from plate 7.5
  35. 35. Epithelium Slide 250 vaginaB.V.B.V. CT SM CT Nerve CT SM SM U-M Histology Collection slide 250.
  36. 36. 10-100µm in diameter Up to 30cm in length 10-15µm in diameter 80-100µm in length 0.2-2µm in diameter 20-200µm in lengthJunquiera. Figure 10-1.
  37. 37. Skeletal Muscle Cardiac Muscle Smooth Muscle Left: Ross and Pawlina. Figure 2 from plate 18. Middle: Unknown Right: Unknown
  38. 38. Muscle Regeneration and GrowthSkeletal Muscle•  Increase in size (hypertrophy)•  Increase in number (regeneration/proliferation) •  Satellite cells are proposed source of regenerative cellsSmooth Muscle•  Increase in size (hypertrophy)•  Increase in number (regeneration/proliferation) •  Smooth muscle cells are proliferative (e.g. uterine myometrium and vascular smooth muscle) •  Vascular pericytes can also provide source of smooth muscleHeart Muscle•  Increase in size (hypertrophy)•  Formerly thought to be non-proliferative •  Post-infarction tissue remodeling by fibroblasts (fibrosis/scarring) •  New evidence suggests mitotic cardiomyocytes and regeneration by blood or vascular-derived stem cells
  39. 39. Skeletal Muscle Satellite Cell Source Undetermined
  40. 40. Activated satellite cell in skeletal muscle Source Undetermined
  41. 41. Learning Objectives1. Be able to identify the three types of muscle at the light and electron microscope levels, including distinctive features of each, such as the intercalated disk of cardiac muscle.2. Be able to describe the structural basis of muscle striation.3. Know the structural elements that harness muscle contraction (i.e., the shortening of myofibrils) to the movement of a body part (i.e., via connection to bone) as well as the mechanism by which muscle cells contract.4.  Understand the function and organization of the connective tissue in muscle (endo-, peri-, and epiysium).5.  Be familiar with the regenerative potential of each muscle type.
  42. 42. Additional Source Information for more information see: http://open.umich.edu/wiki/CitationPolicySlide 5: National Cancer Institute, Wikimedia, http://en.wikipedia.org/wiki/File:Illu_muscle_structure.jpgSlide 6: Source UndeterminedSlide 7: Source UndeterminedSlide 8: Sameerb, Wikipedia, http://en.wikipedia.org/wiki/File:Sarcomere.gifSlide 9: Source UndeterminedSlide 10: Source UndeterminedSlide 12: Source UndeterminedSlide 13: Source UndeterminedSlide 14: Source Undetermined; Dake, Wikipedia, http://en.wikipedia.org/wiki/File:Synapse_diag4.png#fileSlide 15: Source UndeterminedSlide 16: Source UndeterminedSlide 17: Source UndeterminedSlide 19: Source UndeterminedSlide 20: Source UndeterminedSlide 21: Source UndeterminedSlide 22: Source UndeterminedSlide 23: Source UndeterminedSlide 24: Source UndeterminedSlide 25: Source UndeterminedSlide 26: Source UndeterminedSlide 27: Source UndeterminedSlide 28: Source UndeterminedSlide 29: Source UndeterminedSlide 30: Source UndeterminedSlide 31: Source UndeterminedSlide 33: Source UndeterminedSlide 34: Source UndeterminedSlide 35: Source UndeterminedSlide 36: Source UndeterminedSlide 37: Source UndeterminedSlide 39: Source UndeterminedSlide 40: Source Undetermined
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