Future Immunoassay

946 views
751 views

Published on

the Bio bar-code assay

Published in: Technology, Business
0 Comments
1 Like
Statistics
Notes
  • Be the first to comment

No Downloads
Views
Total views
946
On SlideShare
0
From Embeds
0
Number of Embeds
5
Actions
Shares
0
Downloads
0
Comments
0
Likes
1
Embeds 0
No embeds

No notes for slide

Future Immunoassay

  1. 1. Future Immunoassay <ul><li>The Bio bar-code assay </li></ul><ul><ul><li>Miss Pawitra Masa-at </li></ul></ul><ul><ul><li>SIRS/M 4937092 </li></ul></ul>
  2. 2. The Bio bar-code assay <ul><li>The use of DNA as a label for antibodies. </li></ul><ul><li>non-competitive immunoassay. </li></ul><ul><li>Polymerase Chain Reaction (PCR) : amplification of the signal. </li></ul>
  3. 3. Current Enhancement <ul><li>Purpose </li></ul><ul><li>detecting extremely low concentration in serum (attamoles, ) </li></ul><ul><li>Use : </li></ul><ul><li>“ Nanoparticles-Based Bio-Barcode </li></ul><ul><li>for the Ultrasensitive detection of Protein. ” </li></ul>
  4. 4. The system relies on …… <ul><li>Magnetic micro-particle probes with Antibodies that specifically bind a target of interest (Prostate-Specific Antigen (PSA)). </li></ul><ul><li>Nano-particle probes that are encode with DNA that is unique to the protein target of interest. </li></ul><ul><li>2 nd Antibodies that can sandwich the target capture by micro-particle probes. </li></ul>
  5. 5. Why Prostate-Specific Antigen (PSA) ? <ul><li>PSA was chosen as the initial target for these studies because its importance in the detection of prostate and breast cancer. </li></ul><ul><li>PSA as a marker present at low levels. </li></ul>
  6. 6. The Bio-Barcode assay method. <ul><li>Probe design and preparation. </li></ul><ul><li>Nano-particle (NP) probe </li></ul><ul><li>Magnetic Micro-particle (MMP) probe </li></ul>
  7. 8. PSA detection <ul><li>Step 1 : Proteins-Antibodies interaction </li></ul><ul><li>MMP solution + free PSA solution </li></ul><ul><li>Stirred at 30 min </li></ul><ul><li>Place the solution in micro-centrifuge tube seperator at room temp. </li></ul><ul><li>( The MMps-PSA hybrids were concentrate on the wall of tube.) </li></ul><ul><li>After 15 sec. Remove the supernatant (unbound PSA solution) </li></ul><ul><li>Resuspend the MMPs-PSA in Phosphate-Buffered Saline (PBS) ; repeat twice </li></ul><ul><li>Add Nano-particle probes (NPs) to the assay solution. </li></ul><ul><li>(The NPs that provide DNA strands reacted with PSA become </li></ul><ul><li>MMps-PSA-NPs ) </li></ul>
  8. 9. PSA detection cont. <ul><li>Step 2 : Washing step & dehybridization </li></ul><ul><li>Stirred at 30 min. </li></ul><ul><li>Washed with PBS (repeat 4 times each time 1 min). </li></ul><ul><li>Suspend MMps-PSA-NPs in NANO-pure water (2 min). </li></ul><ul><li>(dehybridized bar-code DNA strands from the Nps surface) </li></ul><ul><li>Seperated & collected from the probes with the use of magnetic seperator </li></ul><ul><li>Through Barcode DNA solution to DNA detection system. </li></ul>
  9. 12. DNA Detection Systems.
  10. 13. Advantages of Bio-barcode Method. <ul><li>Because of the target-binding portions is homogeneous. </li></ul><ul><li>Easier & Faster binding between the detection antibody and target analyte. </li></ul><ul><li>Increase sensitivity because the capturing step is more efficient. (can increase capturing by increasing the conc. Of MMPs) </li></ul><ul><li>Because of The use of Nanoparticle probes-barcodes DNA </li></ul><ul><li>Provide high ratio of DNA to label Ab that can increase assay sensitivity </li></ul><ul><li>no need for complicated chemistry for attaching DNA to the surface of NP (simple attaching and washing) </li></ul>
  11. 16. The end…  Thank you 

×