microscopy
One of the most widely used tools in
biological sciences
An instrument that produces an
enlarged image of an object
Mag...
Principle:
The microscope objective first forms in
the tube a magnified aerial image of the
specimen illuminated below th...
What is the standard length of the draw
tube?
160 mm
Which is the most important
component of the microscope?
Objective ...
I. Light microscopes
1. Simple
2. Compound
a. Brightfield
b. Darkfield
c. Phase contrast
d. Ultraviolet
e. Fluorescence
f....
Type of
microscopy
appearance
magnification
use
Brightfield Microbe
stained
1000x Overall
appearance of
most microbes
Dark...
Type of
microscopy
appearance
magnification
use
Ultraviolet UV
absorbing
portion of
cell emitting
light
1000x to
3000x
Cel...
Type of
microscopy
Appearance
magnification
use
TEM Ultrathin
section
showing
cellular
component
s (2-D)
Up to
200,000x
St...
Brightfield Microscope
Dark image
against a
bright or
white
background
Thin, flat components of low
refractile elements and living
cells
polarized light
for birefringent substances
Brightly illuminated specimen
against a dark background
Fluorescent substances
Filters: excitation & barrier
Electron beams &
magnetic lenses
Cm4 microscopy
Cm4 microscopy
Cm4 microscopy
Cm4 microscopy
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Cm4 microscopy

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by : Ms. Crizelda Liwanag

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Cm4 microscopy

  1. 1. microscopy
  2. 2. One of the most widely used tools in biological sciences An instrument that produces an enlarged image of an object Magnification – the increase in an object’s apparent size Resolution – the power of a microscope to clearly show detail
  3. 3. Principle: The microscope objective first forms in the tube a magnified aerial image of the specimen illuminated below the lamp and the condenser.The image is then viewed magnified through the eyepiece. microscope
  4. 4. What is the standard length of the draw tube? 160 mm Which is the most important component of the microscope? Objective lenses If the image remains visible after the exchange of objectives, the lenses are said to be_____ parfocal
  5. 5. I. Light microscopes 1. Simple 2. Compound a. Brightfield b. Darkfield c. Phase contrast d. Ultraviolet e. Fluorescence f. Stereoscopic II. Electron microscopes 1. transmission 2. scanning
  6. 6. Type of microscopy appearance magnification use Brightfield Microbe stained 1000x Overall appearance of most microbes Dark field Light microbe not stained 1000x Overall appearance of most microbes Phase contrast Cellular component s resolved 1000x Resolution of many cellular components
  7. 7. Type of microscopy appearance magnification use Ultraviolet UV absorbing portion of cell emitting light 1000x to 3000x Cellular components that absorb light Fluorescent Microbe fluorescing 1000x to 3000x m-o’s coated w/ dye fluoresce when struck by UV
  8. 8. Type of microscopy Appearance magnification use TEM Ultrathin section showing cellular component s (2-D) Up to 200,000x Study of viruses and detailed structure of larger m-o’s SEM Surface of microbe (3-D) Up to 50,000x Study of surface structure of m-o’s
  9. 9. Brightfield Microscope Dark image against a bright or white background
  10. 10. Thin, flat components of low refractile elements and living cells
  11. 11. polarized light for birefringent substances
  12. 12. Brightly illuminated specimen against a dark background
  13. 13. Fluorescent substances Filters: excitation & barrier
  14. 14. Electron beams & magnetic lenses
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