Cm1 orientation

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Cm1 orientation

  1. 1. CLINICAL MICROSCOPY/UBF 2ndSemesterA.Y.2010-2011
  2. 2. Definition Clinical Microscopy (MTE07) • The course deals with the macroscopic, chemical and microscopic study of the different non-blood body fluids and their by products
  3. 3. Clinical Microscopy • urine, feces, bronchial and bronchoalveolar lavage, sputum, gastric and duodenal contents, pancreatic exocrine secretions, cerebrospinal fluid, synovial fluid, serous fluids (transudates and exudates), sweat, seminal fluid and amniotic fluid. • Collection, handling, processing, reference ranges and clinical significance
  4. 4. PRE-REQUISITES: MICROBIOLGY PHYSIOLOGY COURSE CREDIT: 2 units lec; 1 unit lab
  5. 5. REQUIREMENTS: 1. Color code: YELLOW (LAB) ORANGE (LEC) 2. Orange highlighter and blue ballpen 3. Activity notebook 4. Lecture notes & compilation of hand-outs 5. Portfolio – deadline (final exam) 6. Textbook 7. Quota – processing of specimen and case report, 5 per person (20% of FE) 8. Lab: preliminary reports, post-exam conference, protocols, data sheets 9. PPE; specimen; Group materials 10. Conference – prelims (case ana); SF (quiz show)
  6. 6. QA and Safety • Basic terms • Quality Assurance • Laboratory Safety – Biological hazards – Sharp hazards – Chemical hazards – Radioactive hazards – Electrical hazards – Fire/explosive hazards – Physical hazards
  7. 7. Microscopy • Types of Microscopy • Brightfield Microscope • Care and Use of the Microscope
  8. 8. Renal Function • Renal Anatomy • Renal Blood flow • Renal Physiology – Functions – Processes • Glomerular Filtration • Tubular Reabsorption • Tubular Secretion
  9. 9. Urinalysis • Introduction (History, Urine formation, composition, volume, specimen collection, handling) • Physical examination of urine • Chemical examination of urine • Microscopic examination of urine • Other methods
  10. 10. Renal and Renal-Associated Dses • Glomerular • Tubular • Interstitial • Vascular • Renal Failure • Renal lithiasis • Metabolic disorders
  11. 11. Other Exams for Renal Dses • Basic terms • Urinary sediment Quantification • Stone Analysis • Assessment of GFR • Concentration tests • Tubular Secretory fxn tests
  12. 12. OBF’s • Pregnancy Test and HCG Assay • CSF • Seminal fluid • Synovial fluid • Serous fluid • Amniotic fluid • Sweat • Bronchial washings, bronchoalveolar lavage & sputum • Gastric fluid, Duodenal content and pancreatic exocrine secretion • Fecal analysis
  13. 13. References Brunzel, Nancy A. Fundamentals of Urine and Body Fluid Analysis. W.B. Saunders, Philadelphia. 1994 Henry, John Bernard et al. Clinical Diagnosis by Laboratory Methods. 20th Edition, W.B. Saunders, Philadelphia. 2001 Estridge, Barbara et al. Basic Medical Laboratory Techniques. 4th Edition. Delmar, USA. 2000 Leihmann, Craig A (ed). Saunder's Manual of Clinical Laboratory Science. W.B. Saunders, Philadelphia. 1998
  14. 14. References McBride, Landy J. Textbook of Urinalysis and Body Fluids: A Clinical Approach. Lippincott, Philadelphia. 1998 Sacher, Ronald A & McPherson, Richard A. Widmann's Clinical Interpretation of Laboratory Tests. 11th Edition. F.A. Davis Company, Thailand. 2000 Strasinger, Susan K. & Di Lorenzo, Marjorie S. Urinalysis and Body Fluids. 5th Edition. F.A. Davis Company, Thailand. 2008

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