Bone Presentation Final5 5 2008Presentation Transcript
CONTROL OF IN VITRO TISSUE-ENGINEERED BONE-LIKE STRUCTURES USING HUMAN MESENCHYMAL STEM CELLS AND POROUS SILK SCAFFOLDS Biomaterials 28 (2007) 1152 – 1162 S. Hoffmann, H. Hagenmuller, A. M. Koch, R. Muller, G. Vunjak-Novakovic, D. L. Kaplan, H. P. Merkle, L. Meinel Christian Britt Amy Chaibi Polliana Macedo Niyati Patel Joseph Saltzbart
Presentation Topics/ Agenda:
Cell Types in Remodeling & Healing
Musculoskeletal Injury and Disease
How does the experimental design presented in the article contribute to the understanding / solving of the problem?
1.5 million people suffer fractures in the US (Est.)
Contribution of the Experimental Design to Solving of the Problem
The authors note that “many groups have put effort on controlling the properties of scaffolds made out of various materials, but little work has been devoted to controlling the structure of the in vitro engineered bone (before implantation) on those scaffolds through scaffold design.”
Hoffmann S et al. / Biomaterials 28 (2007) 1152 – 1162.
Scaffold geometry modified outside of the body is hypothesized to optimize bone growth in a novel biomimic approach that seeks to engineer a bone-like tissue in vitro in an attempt to mimic the natural tissue structure.
Tissue Engineering Strategies
Osteoblasts, Chondrocytes, Stem Cells
BMPs, TGF- , IGF I&II, PDGF, Fibroblasts
Spinner Flasks, Rotating, Flow Perfusion
Scaffolds (3-D) - underlying material, macrostructure (special geometry), micro-structure (bulk versus porous), mechanical properties, and degradation characteristics
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