MEDICAL BIOCHEMISTRY:      ENZYMES  MR. GENARO F. ALDERITE        JR,MSERM
ENZYMES- A protein with catalytic properties due to its  power of specific activation
Characteristics of Enzymes1) biological catalysts2) not consumed during a chemical reaction3) speed up reactions from 1000...
Classification of Enzyme                  Specificitya. Absolute specificity: substrate    Succinic dehydrogenase- succini...
Classification of Enzymes:1. According to its composition:    a. Simple enzymes-    b. Complex enzymes          holoenzyme...
2. Class of organic chemical reaction catalyzed:      a. Oxidoreductase - catalyze redox reactions      *dehydrogenases, o...
c. Hydrolases - catalyze hydrolysis reactions    Carbohydrates    1. ptyalin- salivary amylase          -catalyze the hydr...
5. amylopsin- pancreatic amylase             - hydrolysis of starch to dextrins and             maltose *from pancreas toS...
Proteases- catalyze the hydrolysis of derived  proteins and amino acids           1. pepsin- hydrolysis of protein to     ...
Hydrolysis Reaction                β-galactosidaseLactose + H2O                     Glucose + Galactose
d. Lyases - lysis of substrate; produce contains     double bonde. Isomerases - catalyze structural changes;   isomerizati...
Chemical reactions• Chemical reactions need an initial input of  energy = THE ACTIVATION ENERGY• During this part of the r...
Reaction Pathway
Making Reactions Go Faster• Increasing the temperature make molecules move  faster• Biological systems are very sensitive ...
An Enzyme Controlled      Pathway
ENZYMATIC REACTION            PRINCIPLES• Biochemically, enzymes are highly specific for their  substrates and generally c...
CATALYSTS• A catalyst is unaltered during the course of a  reaction and functions in both the forward and  reverse directi...
ENZYMATIC REACTION          PRINCIPLES (cont)• The energy maxima at which the reaction has the  potential for going in eit...
Binding Energy• The graph of activation energy and free energy changes  for an enzymatic reaction also indicates the role ...
Reaction Rates• The rate of the reaction is determined by several factors  including:      A. The concentration of substra...
Effect of Temperature          A reaction rate will generally          increase with increasing          Temperature due t...
Q10 (the temperature coefficient) = the increase inreaction rate with a 10°C rise in temperature.For chemical reactions th...
The effect of temperature                    Q10                DenaturationEnzymeactivity           0   10     20   30   ...
The effect of temperatureFor most enzymes the optimum temperature isabout 30°CMany are a lot lower, cold water fish willdi...
Effect of pH      Variations in pH can affect a      particular enzyme in many ways,      especially if ionizable amino ac...
Extreme pH levels will produce denaturationThe structure of the enzyme is changedThe active site is distorted and the subs...
Optimum pH                     valuesEnzymeactivity                                 Trypsin                               ...
Theories on Enzyme Specificity1. The Lock and Key Hypothesis2. The Induced Fit Hypothesis
The Lock and Key Hypothesis• Fit between the substrate and the active site of the  enzyme is exact• Like a key fits into a...
S                   E       E                            E                                   Enzyme may be                ...
The Induced Fit Hypothesis• Some proteins can change their shape  (conformation)• When a substrate combines with an enzyme...
The Induced Fit HypothesisHexokinase (a) without (b) with glucose substratehttp://www.biochem.arizona.edu/classes/bioc462/...
Hexokinase Active Site:Glucose vs. Galactose Binding
LOCK-AND-KEYINDUCED FIT
Catalytic Mechanisms: Types• Four types of catalytic mechanisms will be  discussed:• binding energy catalysis• general aci...
Acid-Base                                                       CatalysisMany reactions involve the formation of normally ...
Acid-Base Catalysis (cont)• The preceding functional groups can potentially serve  as either proton donors or proton accep...
Binding Energy Catalysis• Binding energy accounts for the overall lowering of  activation energy for a reaction, and it ca...
Covalent Catalysis• This mechanism involves the transient  covalent binding of the substrate to an  amino acid residue in ...
Metal Ion Catalysis• Various metals, all positively charged and  including zinc, iron, magnesium, manganese  and copper, a...
Summary of Catalytic            Mechanisms• In general, more than one type of catalytic  mechanism will occur for a partic...
Inhibitors• Inhibitors are chemicals that reduce the rate of  enzymic reactions.• The are usually specific and they work a...
The effect of enzyme inhibition• Irreversible inhibitors: Combine with the  functional groups of the amino acids in the ac...
Reversible inhibitors: These can be washed out of the solution of enzyme by dialysis.Two Categories:Competitive: These co...
 Non-competitive: These are not influenced by  the concentration of the substrate. It inhibits by  binding irreversibly t...
Medicine inhibitors:a. Methotrexate in cancer chemotherapy to semi-selectively inhibit DNA synthesis of malignantcellsb. A...
Activators: are molecules that          increase activity.Examples: Lipases- Used to assist in the removal of fatty and oi...
Clinical Use of Enzymes• Enzyme Activity in Body Fluids Reflects Organ  Status:• Cells die and release intracellular conte...
Clinical Use of Enzymes (cont)• Enzyme Activity Reflects the Presence of  Inhibitors or Activators• Activity of serum enzy...
Clinical Use of Enzymes (cont)• Enzyme activity can be altered genetically• A mutation in an enzyme can alter its substrat...
Enzymes in the Diagnosis of        PathologyThe measurement of the serum levels ofnumerous enzymes has been shown to be of...
Commonly assayed enzymes :  a.amino transferases:  b. alanine transaminase, ALT (sometimes still  referred to as serum glu...
-The typical liver enzymes measured are AST(aspartate aminotransferase), andALT(Alanine transaminase) .-Normally in liver ...
The 5 types and their normal distribution and levels in  non-disease/injury are listed below. (lactate  dehydrogenase )• L...
• CPK( Creatine phosphokinase) is found primarily in  heart and skeletal muscle as well as the brain.  Therefore, measurem...
Chapter 4 enzymes
Chapter 4 enzymes
Chapter 4 enzymes
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Chapter 4 enzymes

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Chapter 4 enzymes

  1. 1. MEDICAL BIOCHEMISTRY: ENZYMES MR. GENARO F. ALDERITE JR,MSERM
  2. 2. ENZYMES- A protein with catalytic properties due to its power of specific activation
  3. 3. Characteristics of Enzymes1) biological catalysts2) not consumed during a chemical reaction3) speed up reactions from 1000 - 1017, with a mean increase in rate of 00,0004) exhibit stereospecificity --> act on a single stereoisomer of a substrate5) exhibit reaction specificity --> no waste or side reactions
  4. 4. Classification of Enzyme Specificitya. Absolute specificity: substrate Succinic dehydrogenase- succinic acid to fumaric acidb. Linkage specificity:reaction that break bonds Thrombin- acids arginine and glycinec. Reaction specificity: reactions Esterases- hydrolysis of estersd. Group Specificity: compounds chymotrypsin- catalyzes only protein that contains phenylalanine, tryptophan and tyrosine
  5. 5. Classification of Enzymes:1. According to its composition: a. Simple enzymes- b. Complex enzymes holoenzyme - a complete, catalytically active enzyme including all co-factors apoenzyme - the protein portion of a holoenzyme minus the co-factors prosthetic group - a metal or other co- enzyme covalently bound to an
  6. 6. 2. Class of organic chemical reaction catalyzed: a. Oxidoreductase - catalyze redox reactions *dehydrogenases, oxidases, peroxidases, reductases Dehydrogenase-catalyze the removal of H from a substrate Oxidases- activate oxygen so that it will readily c ombine with a substrate b. Transferases - catalyze group transfer reactions; often require coenzymes
  7. 7. c. Hydrolases - catalyze hydrolysis reactions Carbohydrates 1. ptyalin- salivary amylase -catalyze the hydrolysis of starch to dextrin and maltose 2. sucrase- hydrolysis of sucrose to glucosE and fructose - intestinal juices 3. maltase- hydrolysis of maltose to glucose 4. Lactase- hydrolysis of lactose to glucose and galactose
  8. 8. 5. amylopsin- pancreatic amylase - hydrolysis of starch to dextrins and maltose *from pancreas toSintestine* Esters- catalyze the hydrolysis of esters into acidsand alcohol 1. Gastric lipase- hydrolysis of fats to fatty acids and alcohol - part of the gastric juices 2. Steapsin- ( pancreatic lipase) - hydrolysis of fats to fatty acids and
  9. 9. Proteases- catalyze the hydrolysis of derived proteins and amino acids 1. pepsin- hydrolysis of protein to polypeptides 2. trypsin- found in pancreatic juice 3. chymotrypsin
  10. 10. Hydrolysis Reaction β-galactosidaseLactose + H2O Glucose + Galactose
  11. 11. d. Lyases - lysis of substrate; produce contains double bonde. Isomerases - catalyze structural changes; isomerizationf. Ligases - ligation or joining of two substrates with input of energy, usually from ATP hydrolysis; often called synthetases or synthases
  12. 12. Chemical reactions• Chemical reactions need an initial input of energy = THE ACTIVATION ENERGY• During this part of the reaction the molecules are said to be in a TRANSITION STATE
  13. 13. Reaction Pathway
  14. 14. Making Reactions Go Faster• Increasing the temperature make molecules move faster• Biological systems are very sensitive to temperature changes.• Enzymes can increase the rate of reactions without increasing the temperature.• They do this by lowering the activation energy.• They create a new reaction pathway “a short cut”
  15. 15. An Enzyme Controlled Pathway
  16. 16. ENZYMATIC REACTION PRINCIPLES• Biochemically, enzymes are highly specific for their substrates and generally catalyze only one type of reaction at rates thousands and millions times higher than non-enzymatic reactions. Two main principles to remember about enzymes are 1) they act as CATALYSTS (they are not consumed in a reaction and are regenerated to their starting state) and 2) they INCREASE THE RATE of a reaction towards equilibrium (ratio of substrate to product), but they do not determine the overall equilibrium of a reaction.
  17. 17. CATALYSTS• A catalyst is unaltered during the course of a reaction and functions in both the forward and reverse directions. In a chemical reaction, a catalyst increases the rate at which the reaction reaches equilibrium, though it does not change the equilibrium ratio. For a reaction to proceed from starting material to product, the chemical transformations of bond-making and bond-breaking require a minimal threshold amount of energy, termed activation energy. Generally, a catalyst serves to lower the activation energy of a particular reaction.
  18. 18. ENZYMATIC REACTION PRINCIPLES (cont)• The energy maxima at which the reaction has the potential for going in either direction is termed the transition state. In enzyme catalyzed reactions, the same chemical principles of activation energy and the free energy changes (∆Go) associated with catalysts can be applied. Recall that an overall negative ∆Go indicates a favorable reaction equilibrium for product formation. As shown in an enzyme catalyzed reaction, and in the graph, the net effect of the enzyme is to lower the activation energy required for product formation.
  19. 19. Binding Energy• The graph of activation energy and free energy changes for an enzymatic reaction also indicates the role binding energy plays in the overall process. Due to the high specificity most enzymes have for a particular substrate, the binding of the substrate to the enzyme through weak, non-covalent interactions is energetically favorable and is termed binding energy. The same forces important in stabilizing protein conformation (hydrogen bonding and hydrophobic, ionic and van der Waals interactions) are also involved in the stable binding of a substrate to an enzyme.
  20. 20. Reaction Rates• The rate of the reaction is determined by several factors including: A. The concentration of substrate B. Temperature C. pH.
  21. 21. Effect of Temperature A reaction rate will generally increase with increasing Temperature due to increased kinetic energy in the system until a maximal velocity is reached. Above this maximum, the kinetic energy of the system exceeds the energy barrier for breaking weak H-bonds and hydrophobic interactions, thus leading to unfolding and denaturation of the enzyme and a decrease in reaction rate.
  22. 22. Q10 (the temperature coefficient) = the increase inreaction rate with a 10°C rise in temperature.For chemical reactions the Q10 = 2 to 3(the rate of the reaction doubles or triples with every10°C rise in temperature)Enzyme-controlled reactions follow this rule as theyare chemical reactionsBUT at high temperatures proteins denatureThe optimum temperature for an enzyme controlledreaction will be a balance between the Q10 anddenaturation.
  23. 23. The effect of temperature Q10 DenaturationEnzymeactivity 0 10 20 30 40 50 Temperature / °C
  24. 24. The effect of temperatureFor most enzymes the optimum temperature isabout 30°CMany are a lot lower, cold water fish willdie at 30°C because their enzymes denatureA few bacteria have enzymes that can withstandvery high temperatures up to 100°CMost enzymes however are fully denatured at 70°C
  25. 25. Effect of pH Variations in pH can affect a particular enzyme in many ways, especially if ionizable amino acid side chains are involved in binding of the substrate and/or catalysis. Extremes of pH can also lead to denaturation of an enzyme if the ionization state of amino acid(s) critical to correct folding are altered. The effects of pH and temperature will vary for different enzymes and must be determined experimentally.
  26. 26. Extreme pH levels will produce denaturationThe structure of the enzyme is changedThe active site is distorted and the substratemolecules will no longer fit in itAt pH values slightly different from the enzyme’soptimum value, small changes in the charges of theenzyme and it’s substrate molecules will occurThis change in ionisation will affect the binding ofthe substrate with the active site.
  27. 27. Optimum pH valuesEnzymeactivity Trypsin Pepsin 1 3 5 7 9 11 pH
  28. 28. Theories on Enzyme Specificity1. The Lock and Key Hypothesis2. The Induced Fit Hypothesis
  29. 29. The Lock and Key Hypothesis• Fit between the substrate and the active site of the enzyme is exact• Like a key fits into a lock very precisely• The key is analogous to the enzyme and the substrate analogous to the lock.• Temporary structure called the enzyme-substrate complex formed• Products have a different shape from the substrate• Once formed, they are released from the active site• Leaving it free to become attached to another substrate
  30. 30. S E E E Enzyme may be used againEnzyme-substrate Pcomplex P Reaction coordinate
  31. 31. The Induced Fit Hypothesis• Some proteins can change their shape (conformation)• When a substrate combines with an enzyme, it induces a change in the enzyme’s conformation• The active site is then moulded into a precise conformation• Making the chemical environment suitable for the reaction• The bonds of the substrate are stretched to make the reaction easier (lowers activation energy)
  32. 32. The Induced Fit HypothesisHexokinase (a) without (b) with glucose substratehttp://www.biochem.arizona.edu/classes/bioc462/462a/NOTES/ENZYMES/enzyme_mechanism.html
  33. 33. Hexokinase Active Site:Glucose vs. Galactose Binding
  34. 34. LOCK-AND-KEYINDUCED FIT
  35. 35. Catalytic Mechanisms: Types• Four types of catalytic mechanisms will be discussed:• binding energy catalysis• general acid-base catalysis• covalent catalysis• metal ion catalysis
  36. 36. Acid-Base CatalysisMany reactions involve the formation of normally unstable, chargedintermediates. These intermediates can be transiently stabilized in anenzyme active site by interaction of amino acid residues acting as weakacids (proton donors) or weak bases (proton acceptors). The generalacid and general base forms of the most common and best characterizedamino acids involved in these reactions are shown above.
  37. 37. Acid-Base Catalysis (cont)• The preceding functional groups can potentially serve as either proton donors or proton acceptors. This is dependent on many factors including the molecular nature of the substrate, any co-factors involved, and the pH of the active site (which would determine the ionization state of an amino acid side chain). For acid- base catalysis, histidine is the most versatile amino acid due to its pKa which means that in most physiological situations it can act as either a proton donor or proton acceptor. Generally these amino acids will interact together with the substrate, or in conjunction with water or other weak, organic acids and bases found in cells.
  38. 38. Binding Energy Catalysis• Binding energy accounts for the overall lowering of activation energy for a reaction, and it can also be considered as a catalytic mechanism for a reaction. Several catalytic factors in the binding of a substrate and enzyme can be considered: 1) transient limiting of substrate and enzyme movement by reducing the relative motion (or entropy) of the two molecules, 2) solvation disruption of the water shell is thermodynamically favorable, and 3) substrate and enzyme conformational changes. All three of these factors individually or in combination are utilized to some degree by an enzyme. While in some instances these forces alone can account for catalysis, they are frequently components of a complex catalytic process involving factors discussed for the other types of catalytic
  39. 39. Covalent Catalysis• This mechanism involves the transient covalent binding of the substrate to an amino acid residue in the active site. Generally this is to the hydroxyl group of a serine, although the side chains of threonine, cysteine, histidine, arginine and lysine can also be involved.
  40. 40. Metal Ion Catalysis• Various metals, all positively charged and including zinc, iron, magnesium, manganese and copper, are known to form complexes with different enzymes or substrates. This metal- substrate-enzyme complex can aid in the orientation of the substrate in the active site, and metals are known to mediate oxidation- reduction reactions by reversible changes in their oxidation states (like Fe3+ to Fe2+).
  41. 41. Summary of Catalytic Mechanisms• In general, more than one type of catalytic mechanism will occur for a particular enzyme via various combinations of binding energy, acid-base, covalent and metal catalysis. Enzymes as a whole are incredibly diverse in their structures and the types of reactions that they catalyze, therefore there is also a large diversity of catalytic mechanisms utilized, the basis of which must be determined experimentally.
  42. 42. Inhibitors• Inhibitors are chemicals that reduce the rate of enzymic reactions.• The are usually specific and they work at low concentrations.• They block the enzyme but they do not usually destroy it.• Many drugs and poisons are inhibitors of enzymes in the nervous system.
  43. 43. The effect of enzyme inhibition• Irreversible inhibitors: Combine with the functional groups of the amino acids in the active site, irreversibly.Examples: nerve gases and pesticides, containing organophosphorus, combine with serine residues in the enzyme acetylcholine esterase.
  44. 44. Reversible inhibitors: These can be washed out of the solution of enzyme by dialysis.Two Categories:Competitive: These compete with the substrate molecules for the active site. The inhibitor’s action is proportional to its concentration. Resembles the substrate’s structure closely.
  45. 45.  Non-competitive: These are not influenced by the concentration of the substrate. It inhibits by binding irreversibly to the enzyme but not at the active site. Examples• Cyanide combines with the Iron in the enzymes cytochrome oxidase.• Heavy metals, Ag or Hg, combine with –SH groups.T hese can be removed by using a chelating agent such as EDTA.
  46. 46. Medicine inhibitors:a. Methotrexate in cancer chemotherapy to semi-selectively inhibit DNA synthesis of malignantcellsb. Aspirin to inhibit the synthesis ofprostaglandins which are at least partlyresponsible for the aches and pains of arthritisc. Sulfa drugs to inhibit the folic acid synthesisthat is essential for the metabolism and growth ofdisease-causing bacteria
  47. 47. Activators: are molecules that increase activity.Examples: Lipases- Used to assist in the removal of fatty and oily stains. Amylases Detergents- for machine dish washing to remove resistant starch residues. Papaine- To soften meat for cooking.
  48. 48. Clinical Use of Enzymes• Enzyme Activity in Body Fluids Reflects Organ Status:• Cells die and release intracellular contents; increased serum activity of an enzyme can be correlated with quantity or severity of damaged tissues (ex. creatine kinase levels following heart attack)• Increased enzyme synthesis can be induced and release in serum correlates with degree of stimulation (ex. alkaline phosphatase activity as a liver status marker)
  49. 49. Clinical Use of Enzymes (cont)• Enzyme Activity Reflects the Presence of Inhibitors or Activators• Activity of serum enzymes decreases in presence of an inhibitor (ex. some insecticides inhibit serum cholinesterases)• Determine co-factor deficiencies (like an essential vitamin) by enzyme activity (ex. add back vitamin to assay, if activity increases, suggests deficiency in that vitamin)
  50. 50. Clinical Use of Enzymes (cont)• Enzyme activity can be altered genetically• A mutation in an enzyme can alter its substrate affinity, co-factor binding stability etc. which can be used as a diagnostic in comparison with normal enzyme• Loss of enzyme presence due to genetic mutation as detected by increased enzyme substrate and/or lack of product leading to a dysfunction• NOTE: PCR techniques that identify specific messenger RNA or DNA sequences are replacing many traditional enzymatic based markers of genetic disease
  51. 51. Enzymes in the Diagnosis of PathologyThe measurement of the serum levels ofnumerous enzymes has been shown to be ofdiagnostic significance. This is because thepresence of these enzymes in the serumindicates that tissue or cellular damage hasoccurred resulting in the release ofintracellular components into the blood .
  52. 52. Commonly assayed enzymes : a.amino transferases: b. alanine transaminase, ALT (sometimes still referred to as serum glutamate-pyruvate aminotransferase, SGPT) c. aspartate aminotransferase, AST (also referred to as serum glutamate-oxaloacetate aminotransferase, SGOT); d. lactate dehydrogenase, LDH; e. creatine kinase, CK (also called creatine phosphokinase, CPK); f. gamma-glutamyl transpeptidase, GGT.
  53. 53. -The typical liver enzymes measured are AST(aspartate aminotransferase), andALT(Alanine transaminase) .-Normally in liver disease or damage that isnot of viral origin the ratio of ALT/AST is lessthan 1. However, with viral hepatitis the ALT/AST ratio will be greater than 1.
  54. 54. The 5 types and their normal distribution and levels in non-disease/injury are listed below. (lactate dehydrogenase )• LDH 1 – Found in heart and red-blood cells and is 17% – 27% of the normal serum total.• LDH 2 – Found in heart and red-blood cells and is 27% – 37% of the normal serum total.• LDH 3 – Found in a variety of organs and is 18% – 25% of the normal serum total.• LDH 4 – Found in a variety of organs and is 3% – 8% of the normal serum total.• LDH 5 – Found in liver and skeletal muscle and is 0% – 5% of the normal serum total.
  55. 55. • CPK( Creatine phosphokinase) is found primarily in heart and skeletal muscle as well as the brain. Therefore, measurement of serum CPK levels is a good diagnostic for injury to these tissues. The levels of CPK will rise within 6 hours of injury and peak by around 18 hours. If the injury is not persistent the level of CK returns to normal within 2–3 days. Like LDH, there are tissue-specific isozymes of CPK and there designations are described below.• CPK3 (CPK-MM) is the predominant isozyme in muscle and is 100% of the normal serum total.• CPK2 (CPK-MB) accounts for about 35% of the CPK activity in cardiac muscle, but less than 5% in skeletal muscle and is 0% of the normal serum total.• CPK1 (CPK-BB) is the characteristic isozyme in brain and is in significant amounts in smooth muscle and is 0% of the normal serum total.
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