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TREMATODES    Prepared by FZHapan
CHARACTERISTICS   Dorso-ventrally flattened   Unsegmented   Leaf-like   Hermaphroditic except blood flukes   Two radi...
BLOOD FLUKES       Prepared by FZHapan
BLOOD FLUKES   Characteristics     – Dioecious     – Males are shorter and stouter than females     – Lateral margins of ...
BLOOD FLUKES:Schistosomes   Schistosomes    – Schistosoma japonicum : Oriental blood      fluke    – Schistosoma haematob...
Life Cycle             Prepared by FZHapan
Schistosome Miracidium   Miracidium    – Hatches from the egg in      slightly alkaline clean      water with a temp.    ...
Schistosome Sporocystsand Cercaria   Mother sporocysts    develop from    miracidium within    the snail   Daughter spor...
Schistosome Cercaria   Cercaria    – Emerges from      daughter sporocysts    – Escapes from the      snail    – Has a bo...
Schistosomulae   Schistosomule    – Develops from cercaria      after skin penetration    – Adapted to survive in      se...
Schistosoma japonicum   Schistosoma    japonicum    – Life cycle involves      alternating parasitic      stages in mamma...
Schistosoma japonicum   Schistosoma    japonicum    – Primarily parasites of      the portal vein and its      branches  ...
Schistosoma japonicum   Schistosoma    japonicum in    eternal copula    – Males have a      gynecophoral canal      whic...
Schistosoma japonicum   Schistosoma    japonicum Ova    – Ovoidal, rounded or      pear-shaped    – Thin shell    – Pale ...
Schistosoma mansoni   Schistosoma    mansoni male and    female    – Female inside the      gynecophoral canal      of ma...
Schistosoma mansoni   Schistosoma    mansoni male and    female    – Female inside the      gynecophoral canal      of ma...
Schistosoma haematobium   Schistosoma    haematobium adult                   Prepared by FZHapan
Schistosoma haematobium   Schistosoma    haematobium ova    – Note the presence      of terminal spine                   ...
Differentiating Features      of Schistosomes  Feature        S. japonicum                    S. mansoni           S. haem...
Blood Flukes:Pathogenesis and ClinicalManifestations   Schistosomiasis   Host granulomatous reaction to eggs   Pneumoni...
Blood Flukes:                             Schistosomiasis                          Prepared by FZHapan Distended belly is ...
Blood Flukes: Diagnosis   Schistosomiasis    – Eggs may not be demonstrable in the      feces    – Infections where there...
Blood Flukes: Diagnosis   Schistosomiasis    – Stool Examination Techniques         Merthiolate-Iodine Formlin Concentra...
Blood Flukes: Diagnosis   Schistosomiasis         Immunodiagnosis           – Intradermal tests for immediate cutaneous ...
Blood Flukes: Treatment   Treatment    – Praziquantel      (heterocyclic      prazinoisoquinolone      compound)    – Sin...
Blood Flukes:Epidemiology   In the Philippines    – 24 endemic provinces        Sorsogon        Oriental Mindoro       ...
Lung Flukes         Prepared by FZHapan
Lung Flukes Paragonimus westermani Oriental Lung Fluke Paragonimus philippinensis Paragonimus siamensis (cats)        ...
Lung Flukes:Paragonimus westermani   Paragonimus westermani    adult    – Hermaphroditic    – Body covered with spines   ...
Lung Flukes:Paragonimus westermani   Paragonimus    westermani ova    – Yellowish brown    – Thick-shelled    – Operculat...
Lung Flukes:Paragonimus westermaniLife Cycle          Prepared by FZHapan
Lung Flukes:Epidemiology of Paragonimiasis   First Intermediate Host    – Brotia asperata (snail)    – Where miracidium d...
Lung Flukes:Epidemiology of Paragonimiasis   Second Intermediate Host    – Sundathelpusa philippina or Parathelpusa      ...
Lung Flukes:Paragonimus westermani   Man gets infected    after ingestion of    raw or insufficiently    cooked crabs    ...
Lung Flukes:Pathogenesis and ClinicalManifestations   Paragonimiasis    – Cough    – Hemoptysis    – Symptoms      consis...
Lung Flukes:Diagnosis ofParagonimiasis   Radiographs aid in    diagnosis   Definitive diagnosis is    based on the findi...
Lung Flukes:Treatment of Paragonimiasis   Praziquantel    – Drug of choice    – 25 mg/kg body      weight 3x a day for   ...
Lung Flukes:Epidemiology of Paragonimiasis   Has a global distribution   In the Philippines    –   Leyte    –   Sorsogon...
Intestinal Flukes           Prepared by FZHapan
Intestinal Flukes:Fasciolopsis buski   Giant intestinal fluke of man   Parasite of the intestines of humans and    pigs...
Intestinal Flukes:Fasciolopsis buski   Elongated   Oval   20 – 75 mm in length    and 8 -20 mm in width   Covered with...
Intestinal Flukes:Life cycle of Fasciolopsis buski              Prepared by FZHapan
Intestinal Flukes:Pathogenesis of Fasciolopsisbuski   Fasciolopsiasis   Pathological changes caused are:   Traumati c  ...
Intestinal Flukes:Diagnosis Fasciolopsiasis   Detection of    parasite eggs in    stool   Resemble Fasciola    eggs   P...
Intestinal Flukes:Diagnosis Fasciolopsiasis   Detection of    parasite eggs in    stool    – Provided with an      opercu...
Intestinal Flukes:Treatment ofFasciolopsiasis   Praziquantel   25 mg/kg for 3    doses for one day   Side effects:    –...
Intestinal Flukes:Epidemiology of Fasciolopsiasis              Prepared by FZHapan
Intestinal Flukes:Epidemiology of Fasciolopsiasis   Endemic in    –   Southeast Asia    –   China    –   Korea    –   Ind...
Intestinal Flukes:Echinostoma ilocanum   Garrison’s fluke   Echinostomid   There are several species that infect    man...
Intestinal Flukes:Echinostoma ilocanum   Adult    – Reddish gray    – Horse-shape collar of      spines (circum oral disk...
Echinostoma ilocanum showing circum-oral                  disk               Prepared by FZHapan
Intestinal Flukes:Echinostoma ilocanum      Echinostoma ilocanum Life cycle                 Prepared by FZHapan
Intestinal Flukes:Echinostoma ilocanum   Echinostoma    ilocanum Ova    –   Straw-colored    –   Operculated    –   Ovoid...
Intestinal Flukes:Echinostoma ilocanum   First Intermediate Host:    – Gyraulus convexciusculus    – Hippeutis umbilicali...
Intestinal Flukes:Echinostoma ilocanum   Second    Intermediate Host:    – Pila luzonica (kuhol)    – Vivipara angularis ...
Intestinal Flukes:Pathogenesis and ClinicalManifestations Echinostoma ilocanum   Man gets infected when metacercariae in ...
Intestinal Flukes:DiagnosisEchinostoma ilocanum   Detection of    characteristic eggs    in the stool                    ...
Intestinal FlukesTreatmentEchinostoma ilocanum   Praziquantel    – 25 mg/kg for 3 doses for one day    – No alcohol; no f...
Intestinal FlukesEpidemiologyEchinostoma ilocanum   Echinostoma ilocanum is endemic in:    –   Northern Luzon    –   Leyt...
Intestinal FlukesHeterophyids   Many species live in the intestine of    fish-eating hosts:    –   Heterophys heterophyes...
Intestinal FlukesHeterophyids   Mode of transmission is by ingestion    of metacercariae encysted in fish   Metacercaria...
Intestinal FlukesHeterophyids   Heterophyes    heterophyes Adult    – Elongated    – Oval or pyriform    – Measures less ...
Intestinal FlukesHeterophyids   Heterophyes    heterophyes Ova    – Light brown in color    – Ovoid in shape    – Opercul...
Intestinal FlukesHeterophyids         Prepared by FZHapan
Intestinal FlukesHeterophyes heterophyesPathogenesis and ClinicalManifestations   Heterophyiasis   Inflammation at the s...
Intestinal FlukesDiagnosisPathogenesis and ClinicalManifestations   Detection of eggs in the stool using    Kato Katz met...
Intestinal FlukesTreatmentPathogenesis and ClinicalManifestations   Praziquantel    – 25 mg/kg in 3 doses for 1 day      ...
Intestinal FlukesEpidemiologyPathogenesis and ClinicalManifestations   Found in    –   Egypt    –   Greece    –   Israel ...
Liver Flukes          Prepared by FZHapan
Liver FlukesFasciola species   Found in the liver and biliary passages of humans    and ruminants   Fasciola hepatica   ...
Liver FlukesFasciola species   Mode of transmission is by ingestion of    metacercariae found in edible aquatic plants or...
Liver FlukesFasciola hepatica   Adult Worm    – Large, broad, flat      body    – Anterior end forms a      prominent cep...
Liver FlukesFasciola gigantica   Adult Worm    – Larger    – More lanceolate    – Less developed      shoulders (shorter ...
Liver FlukesFasciola hepatica and gigantica   Adult Worm                 Prepared by FZHapan
Liver FlukesFasciola hepatica and gigantica   First Intermediate Host:    – Lymnea philippinensis   Second Intermediate ...
Liver FlukesFasciola hepatica   Fasciola hepatica    Ova    –   Large    –   Ovoid    –   Operculated    –   Bile stained...
Liver FlukesFasciola hepatica   Fasciola gigantica    Ova    – Larger but very      similar to Fasciola      hepatica ova...
Liver FlukesFasciola hepatica   Fasciola gigantica    Ova    – Larger but very      similar to Fasciola      hepatica ova...
Liver FlukesFasciola species                   Prepared by FZHapan
Liver FlukesFasciola speciesPathogenesis and ClinicalManifestations   Fascioliasis   Asymptomatic   Can produce fever ...
Liver FlukesFasciola speciesDiagnosis   Microscopy   Serologic tests    – Low specificity because of cross reactivity   ...
Liver FlukesFasciola speciesTreatment   Bithionol    – 20-50 mg/kg body weight on alternate days to      complete 10 to 5...
Liver FlukesFasciola speciesEpidemiology   Worldwide distribution   Economic importance n livestock    raising   In the...
Liver FlukesClonorchis sinensis   Chinese liver fluke   Oriental Liver Fluke   Distome of China   First intermediate H...
Liver FlukesClonorchis sinensis   Adult Worm    – Narrow, oblong, flat      worm    – Oral sucker is slightly      larger...
Liver FlukesClonorchis sinensis   Ova    –   Bile stained    –   Flask-shaped    –   Operculated    –   Contains a miraci...
Liver FlukesClonorchis sinensisLife Cycle                Prepared by FZHapan
Liver FlukesClonorchis sinensisPathogenesis and ClinicalManifestations Clonorchiasis Provokes intense proliferation of i...
Liver FlukesClonorchis sinensisDiagnosis   Detection of parasite egg in stool   Clonorchis, Opistorchis and    Hetrophyi...
Liver FlukesClonorchis sinensisTreatment   Praziquantel    – 25 mg/kg three times a day for two days    – 60 mg/kg in thr...
Liver FlukesClonorchis sinensisEpidemiology   Transmission is due to consumption of raw,    undercooked fish and salted a...
Pancreatic Fluke          Prepared by FZHapan
Pancreatic FlukeEurytrema pancreaticum   Pancreatic fluke   Stout worm with    ruffled margins   oral sucker is larger ...
Pancreatic FlukeEurytrema pancreaticum   First Intermediate    Host:    – Macrochlamys      indica (snail)   Second    i...
Pancreatic FlukeEurytrema pancreaticum   Ova    –   Operculated    –   Thick shelled    –   Dark brown in color    –   Em...
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Trematodes

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Transcript of "Trematodes"

  1. 1. TREMATODES Prepared by FZHapan
  2. 2. CHARACTERISTICS Dorso-ventrally flattened Unsegmented Leaf-like Hermaphroditic except blood flukes Two radially striated suckers Incomplete digestive tract Adults are covered with spines, except Incomplete digestive tract Most of the body is occupied by reproductive organs Prepared by FZHapan
  3. 3. BLOOD FLUKES Prepared by FZHapan
  4. 4. BLOOD FLUKES Characteristics – Dioecious – Males are shorter and stouter than females – Lateral margins of males are folded ventrally to form a gynecophoral canal in which females are received – Suckers are armed with delicate spines – There is no muscular pharynx – Eggs are non-operculated – Eggs are fully embryonated when laid – Embryonated eggs have a ciliated embryo called miracidium – Cercariae have bifid tails – There is no encysted metacerciarial stage – Infective Stage: cercaria penetrating the unbroken skin Prepared by FZHapan
  5. 5. BLOOD FLUKES:Schistosomes Schistosomes – Schistosoma japonicum : Oriental blood fluke – Schistosoma haematobium: Vesical blood fuke – Schistosoma mansoni: Manson’s blood fluke Prepared by FZHapan
  6. 6. Life Cycle Prepared by FZHapan
  7. 7. Schistosome Miracidium Miracidium – Hatches from the egg in slightly alkaline clean water with a temp. between 25 0C to 310C – Free swimming cilated embryo liberated from the egg – Photactic – Infect snails Prepared by FZHapan
  8. 8. Schistosome Sporocystsand Cercaria Mother sporocysts develop from miracidium within the snail Daughter sporocyst develops from mother sporocyst Cercariae develop from daughter sporocyst Prepared by FZHapan
  9. 9. Schistosome Cercaria Cercaria – Emerges from daughter sporocysts – Escapes from the snail – Has a body and a forked tail – Infects man by skin penetration Prepared by FZHapan
  10. 10. Schistosomulae Schistosomule – Develops from cercaria after skin penetration – Adapted to survive in serum or physiologic saline at 37 0C – Enter the pleural cavity---diaphragm--- peritoneal space--- penetrate the liver to reach the intrahepatic portions of the portal vein Prepared by FZHapan
  11. 11. Schistosoma japonicum Schistosoma japonicum – Life cycle involves alternating parasitic stages in mammalian hosts and free living stages  Egg and miracidium  First stage (mother) sporocyst  Second stage (daughter) sporocyst  cercaria  Schistosomulum  Adult schistosome Prepared by FZHapan
  12. 12. Schistosoma japonicum Schistosoma japonicum – Primarily parasites of the portal vein and its branches – Each female fluke deposits 500-2000 immature eggs/day – Embryonation takes place within 10-12 days – Eggs escape through ulcerations in the intestinal lumen and are passed out with the feces Prepared by FZHapan
  13. 13. Schistosoma japonicum Schistosoma japonicum in eternal copula – Males have a gynecophoral canal which receives the female during copulation Prepared by FZHapan
  14. 14. Schistosoma japonicum Schistosoma japonicum Ova – Ovoidal, rounded or pear-shaped – Thin shell – Pale yellow – Curved hook or spine or lateral knob – Laid in the multicellular stage and embryonte within 10-12 days Prepared by FZHapan
  15. 15. Schistosoma mansoni Schistosoma mansoni male and female – Female inside the gynecophoral canal of male Prepared by FZHapan
  16. 16. Schistosoma mansoni Schistosoma mansoni male and female – Female inside the gynecophoral canal of male Prepared by FZHapan
  17. 17. Schistosoma haematobium Schistosoma haematobium adult Prepared by FZHapan
  18. 18. Schistosoma haematobium Schistosoma haematobium ova – Note the presence of terminal spine Prepared by FZHapan
  19. 19. Differentiating Features of Schistosomes Feature S. japonicum S. mansoni S. haematobiumTestes (male) 6-7 in a single file 8 -9 in a zigzag row 4 -5 in groupsOvary In the middle of the Anterior to the middle of Behind the middle of(Female) body; contains 50 or the body; contains 1-3 the body; contains 20 more eggs eggs – 30 eggsIntestine short longest longEgg Lateral knob Lateral spine Terminal spineIntermediate Oncomelania Biomphalaria and Bulimus (Physopsis)Host AustralorbisDefinitive Man and domestic Man Prepared by FZHapan ManHost animals
  20. 20. Blood Flukes:Pathogenesis and ClinicalManifestations Schistosomiasis Host granulomatous reaction to eggs Pneumonitis due to schistosomula in the lungs Hepatosplenic disease Colonic schistosomiasis Cerebral schistosomiasis Prepared by FZHapan
  21. 21. Blood Flukes: Schistosomiasis Prepared by FZHapan Distended belly is one of the symptoms of schistosomiasis
  22. 22. Blood Flukes: Diagnosis Schistosomiasis – Eggs may not be demonstrable in the feces – Infections where there is scarring prevent passage of eggs into the intestinal lumen Prepared by FZHapan
  23. 23. Blood Flukes: Diagnosis Schistosomiasis – Stool Examination Techniques  Merthiolate-Iodine Formlin Concentration Technique (MIFC) – Sensitive for moderate and heavy infections – Not adequate for light infections (less than 10 eggs/gram of stool)  Kato KatzTechnique – For enumeration of eggs – Most commonly used for evaluating epidemiology, effect of control measures, drug trials Prepared by FZHapan
  24. 24. Blood Flukes: Diagnosis Schistosomiasis  Immunodiagnosis – Intradermal tests for immediate cutaneous hypersensitivity using adult worm extracts – Indirect hemagglutination using adult worm and egg antigens – Circumoval precipitin test – Enzyme-Linked Immunosorbent Assay (ELISA) using soluble antigens of adults and eggs Prepared by FZHapan
  25. 25. Blood Flukes: Treatment Treatment – Praziquantel (heterocyclic prazinoisoquinolone compound) – Single dose of 40- 50 mg/kg – 25 mg/kg in two doses – 20 mg/kg in three doses Prepared by FZHapan
  26. 26. Blood Flukes:Epidemiology In the Philippines – 24 endemic provinces  Sorsogon  Oriental Mindoro  Samar  Leyte  Bohol  All provinces in Mindanao except Misamis Oriental Prepared by FZHapan
  27. 27. Lung Flukes Prepared by FZHapan
  28. 28. Lung Flukes Paragonimus westermani Oriental Lung Fluke Paragonimus philippinensis Paragonimus siamensis (cats) Prepared by FZHapan
  29. 29. Lung Flukes:Paragonimus westermani Paragonimus westermani adult – Hermaphroditic – Body covered with spines – Reddish brown – Measures 4-6 mm in width and 3.5-5 mm in thickness – Resembles a coffee bean – Adult worms are found in pairs or in threes in fibrotic capsules or cysts in the lungs Prepared by FZHapan
  30. 30. Lung Flukes:Paragonimus westermani Paragonimus westermani ova – Yellowish brown – Thick-shelled – Operculated with a thickened abopercular egg – May be seen in the sputum or in feces if the sputum is swallowed Prepared by FZHapan
  31. 31. Lung Flukes:Paragonimus westermaniLife Cycle Prepared by FZHapan
  32. 32. Lung Flukes:Epidemiology of Paragonimiasis First Intermediate Host – Brotia asperata (snail) – Where miracidium develops into 1 sporocyst and 2 redial stages of development Prepared by FZHapan
  33. 33. Lung Flukes:Epidemiology of Paragonimiasis Second Intermediate Host – Sundathelpusa philippina or Parathelpusa grapsoides (former name) – Harbors the metacercaria that is infective to man Prepared by FZHapan
  34. 34. Lung Flukes:Paragonimus westermani Man gets infected after ingestion of raw or insufficiently cooked crabs harboring the metacercariae Prepared by FZHapan
  35. 35. Lung Flukes:Pathogenesis and ClinicalManifestations Paragonimiasis – Cough – Hemoptysis – Symptoms consistent with pulmonary tuberculosis – Misdiagnosed as PTB Prepared by FZHapan
  36. 36. Lung Flukes:Diagnosis ofParagonimiasis Radiographs aid in diagnosis Definitive diagnosis is based on the finding of ova in the sputum, stool or less frequently in aspirated material from abscesses or pleural effusions Multi-dot ELISA Prepared by FZHapan
  37. 37. Lung Flukes:Treatment of Paragonimiasis Praziquantel – Drug of choice – 25 mg/kg body weight 3x a day for three day Bithionol – 15 – 25 mg/kg / day on alternate days for a total of 10-15 days Prepared by FZHapan
  38. 38. Lung Flukes:Epidemiology of Paragonimiasis Has a global distribution In the Philippines – Leyte – Sorsogon – Mindoro – Camarines – Samar – Davao – Cotabato – Basilan Prepared by FZHapan
  39. 39. Intestinal Flukes Prepared by FZHapan
  40. 40. Intestinal Flukes:Fasciolopsis buski Giant intestinal fluke of man Parasite of the intestines of humans and pigs Mode of transmission is by ingestion of encysted metacercariae on aquatic plants The viable metacercariae excyst in the duodenum and becomes mature in about three months Prepared by FZHapan
  41. 41. Intestinal Flukes:Fasciolopsis buski Elongated Oval 20 – 75 mm in length and 8 -20 mm in width Covered with spines No cephalic cone Unbranched intestina caeca which reach up to the posterior end Prepared by FZHapan
  42. 42. Intestinal Flukes:Life cycle of Fasciolopsis buski Prepared by FZHapan
  43. 43. Intestinal Flukes:Pathogenesis of Fasciolopsisbuski Fasciolopsiasis Pathological changes caused are: Traumati c – Inflammation and ulceration Obstructive – Intestinal obstruction due to heavy infection Toxic – Due to absorption of worm metabolites by the host Prepared by FZHapan
  44. 44. Intestinal Flukes:Diagnosis Fasciolopsiasis Detection of parasite eggs in stool Resemble Fasciola eggs Provided with an operculum Prepared by FZHapan
  45. 45. Intestinal Flukes:Diagnosis Fasciolopsiasis Detection of parasite eggs in stool – Provided with an operculum – Large – Unembryonated when laid Resemble Fasciola eggs Prepared by FZHapan
  46. 46. Intestinal Flukes:Treatment ofFasciolopsiasis Praziquantel 25 mg/kg for 3 doses for one day Side effects: – Diziness – Drowsiness – Epigastric pain Prepared by FZHapan
  47. 47. Intestinal Flukes:Epidemiology of Fasciolopsiasis Prepared by FZHapan
  48. 48. Intestinal Flukes:Epidemiology of Fasciolopsiasis Endemic in – Southeast Asia – China – Korea – India Endemicity in the Philippines has not been demonstrated yet Fasciolopsiasis in Filipinos are probably imported cases Prepared by FZHapan
  49. 49. Intestinal Flukes:Echinostoma ilocanum Garrison’s fluke Echinostomid There are several species that infect man There are 2 identified echinostomids that infect man in the Philippines: – Echinostoma ilocanum – Artyfechinostomum malayanum Prepared by FZHapan
  50. 50. Intestinal Flukes:Echinostoma ilocanum Adult – Reddish gray – Horse-shape collar of spines (circum oral disk) around the oral suckers – 49-51 collar spines – Integument is covered by plaque like scales – Simple intestinal caeca Prepared by FZHapan
  51. 51. Echinostoma ilocanum showing circum-oral disk Prepared by FZHapan
  52. 52. Intestinal Flukes:Echinostoma ilocanum Echinostoma ilocanum Life cycle Prepared by FZHapan
  53. 53. Intestinal Flukes:Echinostoma ilocanum Echinostoma ilocanum Ova – Straw-colored – Operculated – Ovoid – Similar to Fasciola and Fasciolopsis buski ova Prepared by FZHapan
  54. 54. Intestinal Flukes:Echinostoma ilocanum First Intermediate Host: – Gyraulus convexciusculus – Hippeutis umbilicalis Prepared by FZHapan
  55. 55. Intestinal Flukes:Echinostoma ilocanum Second Intermediate Host: – Pila luzonica (kuhol) – Vivipara angularis (susong pampang) Prepared by FZHapan Pila luzonica
  56. 56. Intestinal Flukes:Pathogenesis and ClinicalManifestations Echinostoma ilocanum Man gets infected when metacercariae in the second intermediate hosts are ingested Inflammation at the site of attachment of adults Ulceration Diarrhea (sometimes bloody) Abdominal pains General intoxication Prepared by FZHapan
  57. 57. Intestinal Flukes:DiagnosisEchinostoma ilocanum Detection of characteristic eggs in the stool Prepared by FZHapan
  58. 58. Intestinal FlukesTreatmentEchinostoma ilocanum Praziquantel – 25 mg/kg for 3 doses for one day – No alcohol; no fats must be taken 24 hours before and after treatment – Only water must be taken within 3 hours of medication Prepared by FZHapan
  59. 59. Intestinal FlukesEpidemiologyEchinostoma ilocanum Echinostoma ilocanum is endemic in: – Northern Luzon – Leyte – Samar – Mindanao provinces Artyfechinostomum malayanum – First reported in 1987 – Northern and Central Luzon Prepared by FZHapan
  60. 60. Intestinal FlukesHeterophyids Many species live in the intestine of fish-eating hosts: – Heterophys heterophyes – Metagonimus yokogawai – Haplorchis taichui – Haplorchis yokogawai Prepared by FZHapan
  61. 61. Intestinal FlukesHeterophyids Mode of transmission is by ingestion of metacercariae encysted in fish Metacercariae in the abdomen excysts, liberating a larva that attaches to the intestinal wall Prepared by FZHapan
  62. 62. Intestinal FlukesHeterophyids Heterophyes heterophyes Adult – Elongated – Oval or pyriform – Measures less than 2 mm in length – Integument has fine scale-like spines – Some species have gonotyl or genital sucker Prepared by FZHapan
  63. 63. Intestinal FlukesHeterophyids Heterophyes heterophyes Ova – Light brown in color – Ovoid in shape – Operculated – A fully developed symmetrical miracidium is already present – Operculum fits into the egg smoothly – No abopercular protuberance like that of Clonochis sinensis ovum Prepared by FZHapan
  64. 64. Intestinal FlukesHeterophyids Prepared by FZHapan
  65. 65. Intestinal FlukesHeterophyes heterophyesPathogenesis and ClinicalManifestations Heterophyiasis Inflammation at the site of attachment Manifesations are consistent with peptic ulcer (observed among infected individuals in Compostela Valley) – Upper abdominal discomfort – Gurgling abdomen Prepared by FZHapan
  66. 66. Intestinal FlukesDiagnosisPathogenesis and ClinicalManifestations Detection of eggs in the stool using Kato Katz method Care must be taken to distinguish them from Clonorchis and Opistorchis ova Prepared by FZHapan
  67. 67. Intestinal FlukesTreatmentPathogenesis and ClinicalManifestations Praziquantel – 25 mg/kg in 3 doses for 1 day Prepared by FZHapan
  68. 68. Intestinal FlukesEpidemiologyPathogenesis and ClinicalManifestations Found in – Egypt – Greece – Israel – Western India – Central and South China – Japan – Korea – Taiwan – Philippines  Compostela Valley  Mindanao  Emerging public health concern Prepared by FZHapan
  69. 69. Liver Flukes Prepared by FZHapan
  70. 70. Liver FlukesFasciola species Found in the liver and biliary passages of humans and ruminants Fasciola hepatica – Sheep liver fluke – Temperate liver fluke Fasciola gigantica – Giant liver fluke – Tropical liver fluke Prepared by FZHapan
  71. 71. Liver FlukesFasciola species Mode of transmission is by ingestion of metacercariae found in edible aquatic plants or by drinking water with floating metacercariae Metacercariae excsts in the duodenum or jejunum and liberate the juvenile fluke Juvenile fluke penetrates the intestinal wall and reaches the liver capsule The parasite burrows into the liver parenchyma where it grows and develops It becomes sexually mature in the bile ducts Prepared by FZHapan
  72. 72. Liver FlukesFasciola hepatica Adult Worm – Large, broad, flat body – Anterior end forms a prominent cephalic cone – Small oral and ventral suckers – Long and highly branched intestinal caeca Prepared by FZHapan
  73. 73. Liver FlukesFasciola gigantica Adult Worm – Larger – More lanceolate – Less developed shoulders (shorter cephalic cone) – Larger ventral sucker Prepared by FZHapan
  74. 74. Liver FlukesFasciola hepatica and gigantica Adult Worm Prepared by FZHapan
  75. 75. Liver FlukesFasciola hepatica and gigantica First Intermediate Host: – Lymnea philippinensis Second Intermediate Host – Watercress – grass Prepared by FZHapan
  76. 76. Liver FlukesFasciola hepatica Fasciola hepatica Ova – Large – Ovoid – Operculated – Bile stained – unsegmented Prepared by FZHapan
  77. 77. Liver FlukesFasciola hepatica Fasciola gigantica Ova – Larger but very similar to Fasciola hepatica ova *Because of similarities, it is just safe to say Fasciola ova Prepared by FZHapan
  78. 78. Liver FlukesFasciola hepatica Fasciola gigantica Ova – Larger but very similar to Fasciola hepatica ova *Because of similarities, it is just safe to say Fasciola ova Prepared by FZHapan
  79. 79. Liver FlukesFasciola species Prepared by FZHapan
  80. 80. Liver FlukesFasciola speciesPathogenesis and ClinicalManifestations Fascioliasis Asymptomatic Can produce fever Right upper quadrant abdominal pain Hypereosinophilia Acute or invasive phase – Migration from intestine to liver – Traumatic and necrotic lesions in liver parenchyma Chronic or latent phase – Asymptomatic – Parasite has reached the bile ducts – Obstruction – Stimulates inflammation in the biliary epithelium leading to fibrosis Prepared by FZHapan
  81. 81. Liver FlukesFasciola speciesDiagnosis Microscopy Serologic tests – Low specificity because of cross reactivity with antigens of other parasites RFLP – PCR restriction Fragment Length Polymorphism Prepared by FZHapan
  82. 82. Liver FlukesFasciola speciesTreatment Bithionol – 20-50 mg/kg body weight on alternate days to complete 10 to 5 doses Triclabendazole – Also a recommended drug of choice due to:  Efficacy  Safety  Ease of use Prepared by FZHapan
  83. 83. Liver FlukesFasciola speciesEpidemiology Worldwide distribution Economic importance n livestock raising In the Philippines, the dominant species is Fasciola gigantica affecting cattle and water buffalos Few human cases are reported locally Prepared by FZHapan
  84. 84. Liver FlukesClonorchis sinensis Chinese liver fluke Oriental Liver Fluke Distome of China First intermediate Host: – Bulimus fuchsiana (snail not found in the Philippines) Second Intermediate Host: – Ctenopharyngondon idellus (fish) Prepared by FZHapan
  85. 85. Liver FlukesClonorchis sinensis Adult Worm – Narrow, oblong, flat worm – Oral sucker is slightly larger than the ventral sucker – Blind intestinal caeca are simple and extend to the caudal region – Life span is 20-30 years Prepared by FZHapan
  86. 86. Liver FlukesClonorchis sinensis Ova – Bile stained – Flask-shaped – Operculated – Contains a miracidium when oviposited – Does not hatch in water but is ingested with a molluscan host – Has a terminal spine – Electric bulb in shape – Infective to snails only Prepared by FZHapan
  87. 87. Liver FlukesClonorchis sinensisLife Cycle Prepared by FZHapan
  88. 88. Liver FlukesClonorchis sinensisPathogenesis and ClinicalManifestations Clonorchiasis Provokes intense proliferation of intestinal epithelium Acute stage (less than 1 month of infection) – Chills – Fever Chronic stage – Cirrhosis – Portal hypertension Prepared by FZHapan
  89. 89. Liver FlukesClonorchis sinensisDiagnosis Detection of parasite egg in stool Clonorchis, Opistorchis and Hetrophyid ova may not be differentiated under ordinary light microscope ELISA with crude Clonorchis sinensis antigen Enzyme immunoassay (EIA Polymerase Chain Reactions Prepared by FZHapan
  90. 90. Liver FlukesClonorchis sinensisTreatment Praziquantel – 25 mg/kg three times a day for two days – 60 mg/kg in three doses for one day – May be used together with albendazole for light and moderate infections Prepared by FZHapan
  91. 91. Liver FlukesClonorchis sinensisEpidemiology Transmission is due to consumption of raw, undercooked fish and salted and dried fish harboring the metacercariae Over 30 million people are infected in Southeastern Asia No reported cases in children below 10 years old Endemic in: – China – Japan – Korea – Vietnam Prepared by FZHapan
  92. 92. Pancreatic Fluke Prepared by FZHapan
  93. 93. Pancreatic FlukeEurytrema pancreaticum Pancreatic fluke Stout worm with ruffled margins oral sucker is larger than the ventral sucker Prepared by FZHapan
  94. 94. Pancreatic FlukeEurytrema pancreaticum First Intermediate Host: – Macrochlamys indica (snail) Second intermediate Host: – Technomyrmex deterquens (ant) Prepared by FZHapan
  95. 95. Pancreatic FlukeEurytrema pancreaticum Ova – Operculated – Thick shelled – Dark brown in color – Embryonated when laid Prepared by FZHapan
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