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Proposal Presentation
- 1. The Effect of Some Medicinal Plants on the Inhibition of Intestinal Bacteria Natasha M. Delgado Colón RISE Summer Program
- 2. Problem Which medicinal plant- poleo, cinnamon, clavo, ginger or hierbaluisa-will be more effective in the inhibition of the bacteria Escherichia coli, Enterococcusfaecalisand Salmonella enteriditis. 2
- 3. Plants 3
- 4. Objectives Isolate the chemical components of each of the plants. Determine the active component responsible for the inhibition of the bacteria. 4
- 6. hierba luisa
- 7. poleo
- 8. cinnamon
- 9. clavo5
- 11. Canfeno
- 12. Cineol
- 13. Terpineol
- 14. Cariofileno
- 15. Limoneno
- 16. Furocumarins
- 17. Verbenona
- 19. 7 Cinnamon Acids : ascorbic, palmític p-cumérico camfeno, cariofileno, limoneno, linalol Cumarins ( Trunk) eugenol, farnesol, Geraniol cariofileno Furfural (trunk) Alcanfor ( trunk) Taninos ( plant) Mucílagos (trunk)
- 20. 8 Poleo Acids: acético, butírico, fórmico, láurico, palmítico, salicilic Rosmarinico Terpens: pineno, alfa-pineno, beta-pineno, beta-felandreno, cadineno, canfeno, isopulegona, limoneno, mentol, linalol, ocimeno, pulegona, sabineno, terpineol, timol, tuyona Taninos Flavonoides: hesperidina, diosmina
- 21. 9 Ginger - Acids:alfalinolenic, linoleico, ascorbic, aspartic, caprico, caprilico, gadoleico, glutaminic, miristic, oleic, oxalic - Gingerol, citral, citronelal, limonene, canfene, beta-sesquifelandreno, gama-eudesmol, Aminoacids: arginine, asparagine, histidine, isoleucine, leucine, lisine, metionine, niacine,treonine, triptofane, tirosine, valine
- 22. Clavo eugenol ( 90 %) , cariofileno ( 15 %) , furfural, methyl slacilate, pirocatecol, - Cromonas: eugenina, isoeugenitol, isoeugenitina y eugenitina. - Taninos. - Mucilages - Sitosterol - Estigmaterol - Cariofileno - Pineno - Oleanolic acid. 10
- 23. Hypothesis Citral, which is found in ginger and hierbaluisa, will be more effective in the inhibition of the bacteria. 11
- 24. Methodology 12 Plant Extraction Thin Layer Chromatography Autobiography Assay
- 25. 13 Column Chromatography Disk Diffusion Technique
- 26. 08/14/2009 Free template from www.brainybetty.com 14
- 27. Future Goals Natural bactericides Further research in pythocompounds Therapeutic effect 15
- 28. References Yuldashev, M. (2005, January). Flavonoids from the Aerial Part of Viciasubvillosa. Chemistry of Natural Compounds, 41(1), 99-100. Retrieved July 1, 2009, doi:10.1007/s10600-005-0087-x Cho, S., Na, Y., & Ahn, Y. (2003, July). Growth-inhibiting effects of seco-tanapartholides identified in Artemisia princeps var. orientalis whole plant on human intestinal bacteria. Journal of Applied Microbiology, 95(1), 7-12. Retrieved July 1, 2009, doi:10.1046/j.1365-2672.2003.01998.x 16
- 29. References Núñez Meléndez, E. Ph. D. (1892). Plantas Medicinales de Puerto Rico: folklore y fundamentos científicos. San Juan, PR: Editorial de la universidad de Puerto Rico. Benedetti, M. (1999). Sembrando y Sanando en Puerto Rico. Cayey, PR: Verde Luz. 17