Successful detection of 40 COSMIC hotspot mutations at allelic frequency belo...Thermo Fisher Scientific
Research has shown that circulating tumor DNA (ctDNA) is
informative of tumor load and tumor evolution in both solid and
hematological cancer. The ability to detect mutations in ctDNA
holds the promise for an accurate and non-invasive approach to
assess minimum residual disease as well as treatment
response in the future. However, as ctDNA often makes up only
a small fraction of cell-free DNA recovered from the plasma,
traditional methods of targeted sequencing research often face
a poor signal-to-noise ratio that cannot be overcome with deep
coverage.
Here we present a novel research method that is capable of
detecting ultra-rare mutations at allelic frequency below 0.5%.
This approach leverages target multiplexing capabilities of the
Ion AmpliSeq™ technology with some important modifications
to the sample preparation procedures. The new protocol
requires as little as 20 ng of input DNA and offers a sample-toanswer
turn-around time in under 24 hours. To support the
analysis of this new approach, we have further developed a
novel Bayesian statistics that models the propagation of
potential artifacts introduced during amplification and sampling
effects during sequencing to differentiate false positives
(variants observed in sequencing data that were not present in
input DNA) from true mutations that were present at very low
levels in the original research sample.
We successfully applied this new method to detect spike-in
mutant DNA in both cell line (Coriell GM24385) and cfDNA
samples. Specifically, we demonstrated the detection of 140
COSMIC genomic aberrations found in 23 frequently mutated
genes. In preliminary study, the method achieved greater 90%
sensitivity and specificity.
Successful detection of 40 COSMIC hotspot mutations at allelic frequency belo...Thermo Fisher Scientific
Research has shown that circulating tumor DNA (ctDNA) is
informative of tumor load and tumor evolution in both solid and
hematological cancer. The ability to detect mutations in ctDNA
holds the promise for an accurate and non-invasive approach to
assess minimum residual disease as well as treatment
response in the future. However, as ctDNA often makes up only
a small fraction of cell-free DNA recovered from the plasma,
traditional methods of targeted sequencing research often face
a poor signal-to-noise ratio that cannot be overcome with deep
coverage.
Here we present a novel research method that is capable of
detecting ultra-rare mutations at allelic frequency below 0.5%.
This approach leverages target multiplexing capabilities of the
Ion AmpliSeq™ technology with some important modifications
to the sample preparation procedures. The new protocol
requires as little as 20 ng of input DNA and offers a sample-toanswer
turn-around time in under 24 hours. To support the
analysis of this new approach, we have further developed a
novel Bayesian statistics that models the propagation of
potential artifacts introduced during amplification and sampling
effects during sequencing to differentiate false positives
(variants observed in sequencing data that were not present in
input DNA) from true mutations that were present at very low
levels in the original research sample.
We successfully applied this new method to detect spike-in
mutant DNA in both cell line (Coriell GM24385) and cfDNA
samples. Specifically, we demonstrated the detection of 140
COSMIC genomic aberrations found in 23 frequently mutated
genes. In preliminary study, the method achieved greater 90%
sensitivity and specificity.
1. Creating A Number Of Traffic Sources Online
You are going to see that it is going to be important for you to learn how to use one type of
traffic generation in the first place, needless to say to be able to have the best results you
need to continue on to learn other forms of traffic generation as well. Mainly because one
type of traffic generation is working great for you at this time does not mean that it's always
going to be effective, in fact many people discover that traffic can dry up very quickly from
one source. As you continue to read you are going to see that we will be sharing with you a
few different methods which can be very effective at creating traffic.
Something you're going to find that has been very effective for many people for an extremely
long time is using seo techniques in order to produce traffic from the major search engines. I
should mention that this process can still be incredibly effective but simply because google
changes their algorithms so frequently, high ranked sites can lose their ranking overnight. So
in order to make sure that you keep your site ranked as high as you can in google you are
going to have to keep up with the changes that they make and make certain you are
providing them with what they want. One of the most important factors you are going to find
in relation to ranking high in google is whether or not people stay on your internet site for a
long period of time. web hosting
Social media has become an integral part of traffic generation and is a wonderful way to get
your content shared online. When you start sharing your content on the social networks you'll
find that some people will in fact take this content and share it with other people. Should you
have profiles on the main social networks like Facebook, Twitter and LinkedIn, you'll have
more opportunities to interact with your target audience. You're in addition going to see that
you will have the ability to set up certain interest groups on Linkedin and Facebook, and this
can supply you with even more targeted traffic. Once you start creating traffic from the social
networks we mentioned you may want to consider spreading out to all of the other social
networks which are now available.
As technology advances and people's imaginations get better you will discover that there is
really no limit to the ways you are able to generate traffic. One of these newer methods that
men and women have learned can be quite effective is by using something which is called
video marketing and advertising. The relative effect of web hoszting on your situation can be
dramatic and cause issues of all varieties. There are so many possibilities and variations -
twists and turns, that maybe you see how difficult it can be to include all bases. There is a lot,
we know, and that is why we are taking a very short break to state a few words about this.
After all we have read, this is timely and powerful information that should be regarded. As
usual, we typically save the very best for last.