Xenobiotics

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Metabolism of Xenobiotics- A brief review

Metabolism of Xenobiotics- A brief review

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  • 1. BIOTRANSFORMATION/DETOXIFICATIONREACTIONSMETABOLISM OF XENOBIOTICS Biochemistry for Medics http://www.namrata.co/
  • 2. XenobioticsA xenobiotic (Gk xenos "stranger") is acompound that is foreign to the body.Xenobiotics can produce a variety of biologicaleffects including-Pharmacological responsesToxicityImmunological responsesCancers
  • 3. XenobioticsXenobiotics can be-a) Exogenous- The foreign molecules which are not normally ingested or utilized by the organism but they gain entry through dietary food stuffs, or in the form of certain medicines/ drugs used for a therapeutic cause or are inhaled through environment . Examples- Drugs, food additives, pollutants, insecticides, chemical carcinogens etc.
  • 4. XenobioticsXenobiotics can be-b) Endogenous – Though they are not foreign substances but have effects similar to exogenous xenobiotics. These are synthesized in the body or are produced as metabolites of various processes in the body. Examples-Bilirubin, Bile acids, Steroids, Eicosanoids and certain fatty acids.
  • 5. Metabolism of Xenobiotics Metabolism of xenobiotics occurs in two phases- Phase 1, the major reaction involved is hydroxylation. In addition to hydroxylation, a wide range of reactions also take place including- Deamination, Dehalogenation, Desulfuration, Epoxidation, Peroxygenation, and  Reduction
  • 6. Metabolism of XenobioticsPhase 2, the hydroxylated or other compounds producedin phase 1 are converted by specific enzymes to variouspolar metabolites by conjugation with- Glucuronic acid, Sulfate, acetate, Glutathione, or Certain amino acids, or By methylation.
  • 7. Biotransformation/Detoxification ReactionsAll the biochemical reactions involved in the conversion offoreign, toxic and water insoluble molecules to non toxic, watersoluble and excretable forms are called Detoxification /Biotransformation reactionsThe overall purpose of the two phases of metabolism ofxenobiotics is to increase their water solubility (polarity) andthus excretion from the body. In certain situations these reactions may instead increase thetoxicity of a foreign compound, then these arecalled, Entoxification reactions
  • 8. Biotransformation reactions Purpose ◦ Converts lipophilic to hydrophilic compounds ◦ Facilitates excretion Consequences ◦ Changes in solubility characteristics ◦ Detoxification ◦ Metabolic activation
  • 9. Role of Liver  Main organ involved  Hepatocytes contain wide variety of enzymes to process xenobiotics  Enzymes are present in cytosol, endoplasmic reticulum and to lesser extent in other organelles  Each enzyme represents a large family of gene product  Each gene product may be induced by different xenobiotics
  • 10. Overview of biotransformationreactions Phase 1 reactions can limit the toxicity of a drug. Phase 1 reactions can also convert xenobiotics from inactive to biologically active compounds (Metabolic activation). In these instances, the original xenobiotics are referred to as "prodrugs" or "procarcinogens.“ Phase 2/conjugation reactions can convert the active products of phase 1 reactions to less active or inactive species, which are subsequently excreted in the urine or bile. In a very few cases, conjugation may actually increase the biologic activity of a xenobiotic (Metabolic activation).
  • 11. BiotransformationPotentially toxic xenobiotic Relatively harmless Detoxification Metabolic activation Inactive metabolite Reactive intermediate
  • 12. Overview ofdetoxification reactions
  • 13. Comparing Phase I & Phase II
  • 14. Factors affectingBiotransformation of drugs  Prior administration of the drug or Co administration of other drugs  Diet  Hormonal status  Genetics  Disease (e.g., decreased in cardiac and pulmonary disease)  Age and developmental status  Functional status of Liver and Kidney
  • 15. Phase 1 reactions - Overview Phase I reactions include:  Oxidation  Reduction  Hydrolysis reactions They are also called Hydroxylation reactions since they introduce or expose a functional group (e.g., -OH) that serves as the active center for sequential conjugation in a phase II reaction.
  • 16. A) OxidationA large number of foreign substances are destroyed byoxidation in the body.Examples- Oxidation of methyl group containing compoundsMethyl group- is oxidized to acid through formation ofalcohol and aldehydeCH3 CH2OH CHO COOH
  • 17. A) Oxidation Oxidation of Alcohols- Primary aliphatic and aromatic alcohols are oxidized to corresponding acidsMethanol Formaldehyde Formic acidEthanol Acetaldehyde Acetic acidBenzoyal Alcohol Benzaldehyde Benzoic acid
  • 18. Methanol toxicity Methanol has a relatively low toxicity. Methanol is metabolized in the liver.  In the first step of degradation, methanol is transformed to formaldehyde via the enzyme alcohol dehydrogenase (ADH). Transformation of formaldehyde to formic acid via the enzyme aldehyde dehydrogenase is faster  The metabolism of formic acid is very slow; thus, it often accumulates in the body, which results in metabolic acidosis. The major damage occurs to the optic nerve.  Ethanol is given as an antidote, since it is the true substrate of Alcohol dehydrogenase, methanol is spared .
  • 19. Methanol toxicityIt is an example ofEntoxification
  • 20. A) Oxidation  Oxidation of Aromatic Hydrocarbons Aromatic hydrocarbons are oxidized to phenolic compounds, which can further be conjugated with Glucuronic acid or Sulfuric acid in phase 2 reactions so as to be excreted through urine. Benzene Phenol
  • 21. A) OxidationOxidation of AldehydesAldehydes are oxidized to corresponding acid. Acid thusformed is further conjugated in phase 2; e.g. Benzoic acid is conjugated with Glycine to form Hippuricacid.This reaction exclusively takes place in liver. Hippuric acid excretion test is undertaken to determinethe detoxification functions of liver. Benzaldehyde Benzoic Acid
  • 22. A) Oxidation Oxidation of AnilidesAnilides are oxidized to corresponding phenolse.g.- Acetanilide is a constituent of analgesic drug. It isoxidized in the body to form p-Acetyl amino phenol.Acetanilide p-Acetyl -Amino phenol
  • 23. A) Oxidation Oxidation of AminesMany primary aliphatic amines undergo oxidation to formthe corresponding acids and nitrogen is converted tourea.Benzyl amine Benzoic acid + UreaAromatic amines like Aniline is oxidized tocorresponding phenol.
  • 24. A) Oxidation Oxidation of Sulphur containing compoundsThe sulphur present in organic compounds is oxidized toSulphate (SO4-2) Oxidation of DrugsMeprobamate OH- MeprobamateChloral Trichloracetic acid
  • 25. A) OxidationOxidation of certain compounds may result in the production of more toxic compounds (Entoxification). Therefore their formation is prevented.For example-Methanol Formic acidHalogenated Alcohol Halogenated AcidEthylene Glycol Oxalic Acid
  • 26. B) ReductionReduction does not occur extensively in human beingsExamples- Reduction of AldehydesChloral TrichloroethanolTrichloroethanol is excreted after conjugation with D-Glucuronic acid as corresponding glucuronide.
  • 27. B) Reduction Reduction of Nitro compoundsp- nitrobenzene p- Amino benzenep- nitro phenol p-Aminophenol p- nitro phenol
  • 28. C)HydrolysisCertain therapeutic compounds undergo hydrolysis,Examples-Acetyl Salicylic acid Acetic acid + Salicylic acidAtropine Tropic acid + TropineDigitalis Sugar + AglyconeProcaine p- Amino Benzoic acid + Diethyl amino ethanol
  • 29. C)Hydrolysis
  • 30. Phase 1 reactions- Enzymes Mainly Catalyzed by members of a class of enzymes referred to as Monooxygenases, Mixed Function oxidases or Cytochrome P450s. Other enzymes of significance are- o Aldehyde and alcohol dehydrogenase o Deaminases o Esterases o Amidases o Epoxide hydrolases
  • 31. Cytochrome P450Enzyme systemThe reaction catalyzed by a monooxygenase (cytochromeP450) is as follows: RH above can represent a very wide variety ofxenobiotics, includingdrugs, carcinogens, pesticides, petroleum products, andpollutants (such as a mixture of PCBs). In addition, endogenous compounds, such as certainsteroids, Eicosanoids, fatty acids, and retinoids, are alsosubstrates.The substrates are generally lipophilic and are renderedmore hydrophilic by hydroxylation.
  • 32. Properties of HumanCytochrome P450s Involved in phase I of the metabolism of innumerablexenobiotics Involved in the metabolism of many endogenouscompounds All are haemoproteins Exhibit broad substrate specificity, thus act on manycompounds Extremely versatile catalysts, perhaps catalyze about60 types of reactions Basically they catalyze reactions involvingintroduction of one atom of oxygen into thesubstrate and one into water
  • 33. Properties of HumanCytochromeP450s(Contd.) Liver contains highest amounts, but found in most if notall tissues, including small intestine, brain, and lung Located in the smooth endoplasmic reticulum or inmitochondria (steroidogenic hormones) In some cases, their products are mutagenic orcarcinogenic Many have a molecular mass of about 55 kDa Many are inducible, resulting in one cause of druginteractions Many are inhibited by various drugs or their metabolicproducts, providing another cause of drug interactions Some exhibit genetic polymorphisms, which canresult in atypical drug metabolism
  • 34. Phase 2 - Conjugation Conjugation is a process by which the foreign molecules andtheir metabolites are coupled with a conjugating agent and areconverted to soluble, non toxic derivatives which are easilyexcreted in urine Conjugation reactions can occur independently or canfollow phase 1(hydroxylation) reactions Conjugation takes place primarily in liver but can occur inkidney also After conjugation the products are generally rendered nontoxic but in certain conditions they are left unchanged orbecome more toxic.
  • 35. Types of Phase 2 Reactions1. Glucuronidation2. Sulfation3. Acetylation4. Methylation5. Conjugation with Amino acids6. Conjugation with G-SH
  • 36. 1) GlucuronidationGlucuronidation is the most frequent conjugationreaction. UDP-glucuronic acid , is the Glucuronyl donor, whichis formed in the uronic acid pathway of Glucosemetabolism Glucuronosyl transferases, present in both theendoplasmic reticulum and cytosol, are the catalysts.Theglucuronide may be attached tooxygen, nitrogen, or sulfur groups of the substrates.
  • 37. 1) GlucuronidationCompounds conjugated with Glucuronic acid are-1) Bilirubin2) Aromatic acids- Benzoic acid3) Phenols, Secondary and Tertiary aliphatic alcohols4) Antibiotics like Chloramphenicol5) Hormones- Thyroid hormone, derivatives of corticosteroids and sex hormone metabolites6) 2-Acetylaminofluorene (a carcinogen)7) Aniline8) Meprobamate (a tranquilizer)
  • 38. GlucuronidationGlucuronidation of Bilirubin UDP- G dehydrogenase1) UDP Glucose UDP Glucuronic acid 2 NAD+ 2 NADH +2H+2) UDP Glucuronic acid + Bilirubin UDP- Glucuronyl TransferaseBilirubin Monoglucuronide +UDP
  • 39. Glucuronidation Glucuronidation of Bilirubin Bilirubin Monoglucuronide+ UDP Glucuronic acid UDP- Glucuronyl Transferase Bilirubin Diglucuronide + UDP Most of the bilirubin excreted in the bile of mammals is in the form of bilirubin diglucuronide. Bilirubin-UGT activity can be induced by a number of clinically useful drugs, including Phenobarbital.
  • 40. 2) Sulfation The sulfate donor is adenosine 3-phosphate-5-phosphosulfate (PAPS) this compound is called "activesulfate― The enzyme is sulfo transferase Compounds which are conjugated with sulphate are asfollows-o Phenolso Cresolso Indoleo Steroidso Oestrogen and Androgenso Tyrosine to form Tyrosine-O- Sulphate, which is required forthe formation of Fibrinogeno Glycosaminoglycans, glycolipids, and glycoproteins
  • 41. 2) Sulfation Active Sulfate Phenol Phenyl Sulfuric AcidSulfotransferases are localized in the cytosol and transfersulphate moiety mainly to OH group. The donor of sulphate isPAPS(Active sulphate) which is synthesized from 2 mol of ATPand one mol of sulphate.
  • 42. 3) Acetylation Acetylation is represented bywhere X represents a xenobiotic. Acetyl-CoA (active acetate) is the acetyl donor.These reactions are catalyzed by acetyltransferasespresent in the cytosol of various tissues, particularly liver Polymorphic types of acetyltransferases exist, resultingin individuals who are classified as slow or fastacetylators, and influence the rate of clearance of drugsfrom blood.Slow acetylators are more subject to certain toxic effects ofdrug because the drug persists longer in these individuals.
  • 43. 3) AcetylationCompounds conjugated by Acetylation- Sulphanilamide PABA (Para Amino Benzoic Acid) Isoniazid Acetyl co A Condensing Enzyme PABA Acetylated PABA
  • 44. 4) Methylation  Methylation is limited in the body  S- Adenosyl Methionine (Active Methionine )acts as a Methyl group donor  Reactions are called Transmethylation reactions  Enzymes catalyzing the reactions are Methyl transferases
  • 45. 4) MethylationCompounds conjugated by Methylation are- Nicotinamide CH3Nicotinamide N- Methyl Nicotinamide p- Methyl Amino Azo benzene CH3 p- Dimethyl Amino Azo Benzene (Hepatic Carcinogen) O- Methylation ofestrogen, norepinephrine, epinephrine and theirmetabolites.
  • 46. 5) Conjugation withAmino acids1) Conjugation with Glycine Benzoic acid + Glycine Hippuric acid Nicotinamide + Glycine Nicotinuric Acid Cholic and deoxy Cholic acid are conjugated toform Glyco cholic acid and Glycodeoxy cholic acid
  • 47. 5) Conjugation withAmino acids2) Conjugation with CysteineA few aromatic compounds are conjugated with Cysteine inthe presence of Acetic acid to form Mercapturic acidBromo Benzene + Cysteine + Acetic acid Bromo phenyl Mercapturic acid Naphthalene + Cysteine + Acetic Acid Naphthyl Mercapturic acid
  • 48. 5) Conjugation withAmino acids3) Conjugation with GlutaminePhenyl Acetic acid + GlutaminePhenyl Acetyl GlutamineThis reaction is important in patients of Phenylketonuria, since excess of Phenyl acetyl glutamine isexcreted in urine, that imparts a mousy odor to the urine.
  • 49. 6)Conjugation withGlutathioneGlutathione (Υ-glutamyl-cysteinylglycine) is a tripeptideconsisting of glutamic acid, cysteine, and glycine Glutathione is commonly abbreviated GSH (because ofthe sulfhydryl group of its cysteine, which is the businesspart of the molecule). A number of potentially toxic electrophilic xenobiotics(such as certain carcinogens) are conjugated to thenucleophilic GSH in reactions that can be represented asfollows:where R = an electrophilic xenobiotic.
  • 50. 6)Conjugation withGlutathione reactions are called The enzymes catalyzing these  glutathione S-transferases A variety of glutathione S-transferases are present in human tissue. They exhibit different substrate specificities and can be separated by electrophoretic and other techniques. Ifthe potentially toxic xenobiotics were not conjugated to GSH, they would be free to combine covalently with DNA, RNA, or cell protein and could thus lead to serious cell damage. GSH is therefore an important defense mechanism against certain toxic compounds, such as some drugs and carcinogens.
  • 51. Effects of Xenobiotics
  • 52. Effects of XenobioticsMetabolism of a xenobiotic can result in cellinjury, immunologic damage, or cancer. Cell injury (cytotoxicity), can be severe enough toresult in cell death.These macromolecular targets include DNA, RNA, andprotein.The reactive species of a xenobiotic may bind to aprotein, altering its antigenicityThe resulting antibodies can then damage the cell byseveral immunologic mechanisms that grossly perturbnormal cellular biochemical processes.
  • 53. Effects of XenobioticsReactions of activated species of chemical carcinogenswith DNA are of great importance in chemicalcarcinogenesis Some chemicals (eg, benzo[α]pyrene) requireactivation by monooxygenases in the endoplasmicreticulum to become carcinogenic (they are thus calledindirect carcinogens). The products of the action of certain monooxygenaseson some procarcinogen substrates are epoxides. Epoxides are highly reactive and mutagenic orcarcinogenic or both. Epoxide hydrolase—like cytochrome P450acts on thesecompounds, converting them into much less reactivedihydrodiols.
  • 54. SummaryXenobiotics are chemical compounds foreign to thebody, such as drugs, food additives, and environmentalpollutants Xenobiotics are metabolized in two phases. The majorreaction of phase 1 is hydroxylation catalyzed by a varietyof monooxygenases, also known as the cytochromeP450s. In phase 2, the hydroxylated species areconjugated with a variety of hydrophilic compounds suchas glucuronic acid, sulfate, or glutathione. The combinedoperation of these two phases renders lipophiliccompounds into water-soluble compounds that can beeliminated from the body. Xenobiotics can produce a variety of biologiceffects, including pharmacologicresponses, toxicity, immunologic reactions, and cancer.