Chromatography

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Chromatography

  1. 1. CHROMATOGRAPHY Biochemistry of Medics 1
  2. 2. Chromatography Biochemistry of Medics 2
  3. 3. Chromatography Biochemistry of Medics 3
  4. 4. Chromatography- Principle Biochemistry of Medics 4
  5. 5. Chromatography- Classification Biochemistry of Medics 5
  6. 6. Chromatography- Classification(Contd.) Biochemistry of Medics 6
  7. 7. Partition Chromatography Biochemistry of Medics 7
  8. 8. Paper chromatography Biochemistry of Medics 8
  9. 9. Paper chromatography Biochemistry of Medics 9
  10. 10. Paper chromatography- Procedure Biochemistry of Medics 10
  11. 11. Paper chromatography- Procedure Biochemistry of Medics 11
  12. 12. Ascending and Descending Paper Chromatography Biochemistry of Medics 12
  13. 13. Paper Chromatography -Analysis Biochemistry of Medics 13
  14. 14. Rƒ value Biochemistry of Medics 14
  15. 15. Two dimensional chromatography Biochemistry of Medics 15
  16. 16. Significance of Paper chromatography Biochemistry of Medics 16
  17. 17. Thin layer chromatography (TLC) Biochemistry of Medics 17
  18. 18. TLC- Procedure Biochemistry of Medics 18
  19. 19. TLC -Overview Biochemistry of Medics 19
  20. 20. Significance of TLC Biochemistry of Medics 20
  21. 21. Advantages of TLC over paper chromatography Biochemistry of Medics 21
  22. 22. 2)Adsorption chromatography Biochemistry of Medics 22
  23. 23. 2)Adsorption chromatography- Elution Biochemistry of Medics 23
  24. 24. Adsorption chromatographyThe substance loosely adsorbed to the stationary medium comes in the earlier fraction. Biochemistry of Medics 24
  25. 25. Ion – Exchange chromatography Biochemistry of Medics 25
  26. 26. Ion – Exchange chromatography Biochemistry of Medics 26
  27. 27. Ion – Exchange chromatography- Procedure Biochemistry of Medics 27
  28. 28. Ion – Exchange chromatography-Procedure (Contd.) Biochemistry of Medics 28
  29. 29. Types of ion exchanges resins Biochemistry of Medics 29
  30. 30. Ion – Exchange chromatography- An Overview Biochemistry of Medics 30
  31. 31. 4) Gel filtration chromatography orMolecular Sieve chromatography Biochemistry of Medics 31
  32. 32. Gel filtration chromatography orMolecular Sieve chromatography Biochemistry of Medics 32
  33. 33. Gel filtration chromatography-AnOverview Larger particles come out first, while smaller particles come in later fractions Biochemistry of Medics 33
  34. 34. 5) Affinity chromatography Biochemistry of Medics 34
  35. 35. Affinity chromatography- Significance Biochemistry of Medics 35
  36. 36. Affinity chromatography- SignificanceBy using antibodies, antigens can be easily separated.Conversely, antibodies can be purified by passing through a columncontaining the antigen. Specific Antibody binds to a specific antigen attached to the stationary medium, theremaining antibodies come out in earlier fractions. Biochemistry of Medics 36
  37. 37. 6) High performance liquid chromatography- Biochemistry of Medics 37
  38. 38. High performance liquid chromatography- Biochemistry of Medics 38
  39. 39. High performance liquid chromatography- Significance Biochemistry of Medics 39
  40. 40. High performance liquidchromatography- Apparatus Biochemistry of Medics 40
  41. 41. 6) Gas liquid chromatography- Biochemistry of Medics 41
  42. 42. Gas liquid chromatography- Biochemistry of Medics 42
  43. 43. Gas liquid chromatography-Apparatus Biochemistry of Medics 43
  44. 44. Summary Biochemistry of Medics 44

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