Chromatography
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Chromatography

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    Chromatography Chromatography Presentation Transcript

    • CHROMATOGRAPHY Biochemistry of Medics 1
    • Chromatography Biochemistry of Medics 2
    • Chromatography Biochemistry of Medics 3
    • Chromatography- Principle Biochemistry of Medics 4
    • Chromatography- Classification Biochemistry of Medics 5
    • Chromatography- Classification(Contd.) Biochemistry of Medics 6
    • Partition Chromatography Biochemistry of Medics 7
    • Paper chromatography Biochemistry of Medics 8
    • Paper chromatography Biochemistry of Medics 9
    • Paper chromatography- Procedure Biochemistry of Medics 10
    • Paper chromatography- Procedure Biochemistry of Medics 11
    • Ascending and Descending Paper Chromatography Biochemistry of Medics 12
    • Paper Chromatography -Analysis Biochemistry of Medics 13
    • Rƒ value Biochemistry of Medics 14
    • Two dimensional chromatography Biochemistry of Medics 15
    • Significance of Paper chromatography Biochemistry of Medics 16
    • Thin layer chromatography (TLC) Biochemistry of Medics 17
    • TLC- Procedure Biochemistry of Medics 18
    • TLC -Overview Biochemistry of Medics 19
    • Significance of TLC Biochemistry of Medics 20
    • Advantages of TLC over paper chromatography Biochemistry of Medics 21
    • 2)Adsorption chromatography Biochemistry of Medics 22
    • 2)Adsorption chromatography- Elution Biochemistry of Medics 23
    • Adsorption chromatographyThe substance loosely adsorbed to the stationary medium comes in the earlier fraction. Biochemistry of Medics 24
    • Ion – Exchange chromatography Biochemistry of Medics 25
    • Ion – Exchange chromatography Biochemistry of Medics 26
    • Ion – Exchange chromatography- Procedure Biochemistry of Medics 27
    • Ion – Exchange chromatography-Procedure (Contd.) Biochemistry of Medics 28
    • Types of ion exchanges resins Biochemistry of Medics 29
    • Ion – Exchange chromatography- An Overview Biochemistry of Medics 30
    • 4) Gel filtration chromatography orMolecular Sieve chromatography Biochemistry of Medics 31
    • Gel filtration chromatography orMolecular Sieve chromatography Biochemistry of Medics 32
    • Gel filtration chromatography-AnOverview Larger particles come out first, while smaller particles come in later fractions Biochemistry of Medics 33
    • 5) Affinity chromatography Biochemistry of Medics 34
    • Affinity chromatography- Significance Biochemistry of Medics 35
    • Affinity chromatography- SignificanceBy using antibodies, antigens can be easily separated.Conversely, antibodies can be purified by passing through a columncontaining the antigen. Specific Antibody binds to a specific antigen attached to the stationary medium, theremaining antibodies come out in earlier fractions. Biochemistry of Medics 36
    • 6) High performance liquid chromatography- Biochemistry of Medics 37
    • High performance liquid chromatography- Biochemistry of Medics 38
    • High performance liquid chromatography- Significance Biochemistry of Medics 39
    • High performance liquidchromatography- Apparatus Biochemistry of Medics 40
    • 6) Gas liquid chromatography- Biochemistry of Medics 41
    • Gas liquid chromatography- Biochemistry of Medics 42
    • Gas liquid chromatography-Apparatus Biochemistry of Medics 43
    • Summary Biochemistry of Medics 44