Biochemistry quiz 3
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spot questions, clinical problems, instruments, techniques

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Biochemistry quiz 3 Presentation Transcript

  • 1. Biochemistry Quiz- 3 Biochemistry For Medics www.namrata.co3/13/2013 Biochemistry For Medics 1
  • 2. Identify the disease3/13/2013 Biochemistry For Medics 2
  • 3. Answer• Palmar Xanthomas3/13/2013 Biochemistry For Medics 3
  • 4. Breath Hydrogen• Breath Hydrogen test is done for the diagnosis of-------------------------- ?3/13/2013 Biochemistry For Medics 4
  • 5. Answer• Lactose intolerance3/13/2013 Biochemistry For Medics 5
  • 6. Identify the structure andmention its use3/13/2013 Biochemistry For Medics 6
  • 7. Answer• Biotin• Participates in “Carboxylation reactions”3/13/2013 Biochemistry For Medics 7
  • 8. Identify the technique andmention its use3/13/2013 Biochemistry For Medics 8
  • 9. Answer• DNA foot printing• For identification of promoter regions on DNA3/13/2013 Biochemistry For Medics 9
  • 10. Identify the instrument3/13/2013 Biochemistry For Medics 10
  • 11. Answer• Blood Gas Analyzer3/13/2013 Biochemistry For Medics 11
  • 12. What is the normal referencerange for CK?3/13/2013 Biochemistry For Medics 12
  • 13. Answer• 10-50 IU/L3/13/2013 Biochemistry For Medics 13
  • 14. Name the enzyme for thesecond reaction3/13/2013 Biochemistry For Medics 14
  • 15. Answer• UDP –Glucuronyl Transferase3/13/2013 Biochemistry For Medics 15
  • 16. What is the underlyingdefect?3/13/2013 Biochemistry For Medics 16
  • 17. Answer• Its Trousseau‟s sign, an indication of latent tetany3/13/2013 Biochemistry For Medics 17
  • 18. What do you expect theplasma concentration ofcholesterol in this patient ?3/13/2013 Biochemistry For Medics 18
  • 19. Answer• Higher than normal• It‟s a case of Hypothyroidism.3/13/2013 Biochemistry For Medics 19
  • 20. Which type of fatty acid is usedfor esterification of cholesterolby ACAT ?3/13/2013 Biochemistry For Medics 20
  • 21. Answer• Mono unsaturated fatty acid3/13/2013 Biochemistry For Medics 21
  • 22. Name one precipitating factorfor the disease3/13/2013 Biochemistry For Medics 22
  • 23. Answer• Alcohol• It is gouty arthritis3/13/2013 Biochemistry For Medics 23
  • 24. Ribozyme ???• Give one example of RNA acting as an enzyme.3/13/2013 Biochemistry For Medics 24
  • 25. Answer• Peptidyl Transferase in translation3/13/2013 Biochemistry For Medics 25
  • 26. Use of AMP ?• Give an example where AMP part of ATP is incorporated in to the substrate.3/13/2013 Biochemistry For Medics 26
  • 27. Answer• Conversion of FMN to FAD3/13/2013 Biochemistry For Medics 27
  • 28. What is the significance ofred line in this diagram?3/13/2013 Biochemistry For Medics 28
  • 29. Answer• It indicates shine Delgarno sequence• The small subunit/IF3 complex binds to the mRNA. Specifically, it binds to the sequence AGGAGG, known as the Shine-Delgarno sequence, which is found in all prokaryotic mRNAs.( Its required for the initiation of protein synthesis)3/13/2013 Biochemistry For Medics 29
  • 30. RNA editing ? .The central dogma states that for a given gene and geneproduct there is a linear relationship between the codingsequence in the DNA, the m- RNA sequence, and theprotein sequence.Is there any exception to the rule if so then giveexample and signify the purpose of this change ?3/13/2013 Biochemistry For Medics 30
  • 31. Answer• Editing of Apo – B m- RNA in the intestine• Apo B 48 is a component of chylomicrons3/13/2013 Biochemistry For Medics 31
  • 32. IDENTIFY THE INSTRUMENT3/13/2013 Biochemistry For Medics 32
  • 33. Answer• HPLC- High Performance Liquid Chromatography for the separation or purification of Biomolecules.3/13/2013 Biochemistry For Medics 33
  • 34. MEGXWhat is the full form ?What is the significance ?3/13/2013 Biochemistry For Medics 34
  • 35. Answer1. MEGX Test (Mono ethyl Glycine xylidine) is a liver function test based on detoxifying functions of liver.3/13/2013 Biochemistry For Medics 35
  • 36. What will the effect on RenalGlutaminase activity in thispatient ?3/13/2013 Biochemistry For Medics 36
  • 37. Answer• It is signifying Respiratory Acidosis. Kidneys will tend to conserve bicarbonates and excretion of H+ will increase, thus the glutaminase activity will increase.3/13/2013 Biochemistry For Medics 37
  • 38. Which complex in ElectronTransport Chain does nothave the Fe S center?3/13/2013 Biochemistry For Medics 38
  • 39. Answer Complex IV- (Cytochrome a-a3 oxidase complex)3/13/2013 Biochemistry For Medics 39
  • 40. PROBLEM• The hypothetical „stimulin‟ gene contains two exons that encode a protein of 100 amino acids. They are separated by an intron of 100 bp beginning after the codon for amino acid 10. Stimulin messenger RNA has 5‟ and 3‟ untranslated regions of 70 and 30 nucleotides respectively. A complementary DNA made from mature stimulin RNA would have how many bp?3/13/2013 Biochemistry For Medics 40
  • 41. Answer• 400 bp3/13/2013 Biochemistry For Medics 41
  • 42. Problem• Which of the following involves Isoprenoids ?• a) The chromophore of visual pigment• b) Carnitine• c) Thiamine• d) Ketone bodies3/13/2013 Biochemistry For Medics 42
  • 43. Answera) The chromophore of visual pigment3/13/2013 Biochemistry For Medics 43
  • 44. Problem• What is the defect in Hereditary Non polyposis colon cancer (HNPCC)?3/13/2013 Biochemistry For Medics 44
  • 45. Answer• Faulty mismatch repair3/13/2013 Biochemistry For Medics 45
  • 46. Problem• Name one molybdenum requiring enzyme.3/13/2013 Biochemistry For Medics 46
  • 47. Answer• Xanthine oxidase3/13/2013 Biochemistry For Medics 47
  • 48. Problem• Chitin is a polymer of ?3/13/2013 Biochemistry For Medics 48
  • 49. Answer• The repeating unit in Chitin is N-Acetyl Glucosamine.3/13/2013 Biochemistry For Medics 49
  • 50. What is this structure?3/13/2013 Biochemistry For Medics 50
  • 51. Answer• β meander- Super secondary protein structure3/13/2013 Biochemistry For Medics 51
  • 52. What is the role of this vitamin inGlycogen metabolism ?3/13/2013 Biochemistry For Medics 52
  • 53. Answer• It acts as a prosthetic group for the Muscle Phosphorylase enzyme.• So helps in muscle glycogenolysis.3/13/2013 Biochemistry For Medics 53
  • 54. What is the Reichert- Meissl Number for Butter ?3/13/2013 Biochemistry For Medics 54
  • 55. Answer• Butter has high Reichert- Meissl Number and is the only fat with high number3/13/2013 Biochemistry For Medics 55