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  • 1.  
  • 2.  
  • 3. Introduction
  • 4. Structure
    • N-terminal domain
    • Containing different numbers of zinc fingers.
    • Exhibit DNA binding activity when it has 3 or more zinc fingers .
    • Bind to DNA sites contain the core motif
    • GGGA and ZNFs 2,3
    • C -terminal domain
    • Oligomerization domain.
    • Containing a bipartite transactivation domain and 2 zinc finger motifs.
    • Mediate hommo or hetrodimerisation between isoforms and iteract in with other molecules .
  • 5.  
  • 6. Isoforms
  • 7.  
  • 8. ikaros gene expression in human non-lymphoid leukemia cell line
  • 9.  
  • 10. Different roles
  • 11.  
  • 12. Disease associated with ikaros
  • 13. Function of Ikaros
    • Ikaros appears function both as a transcriptional repressor and as an activator through its ability to bind a large number of nuclear factors:
    • Histon deacetylase repressor complexses SIN3 , NURD (causes gene inactivation )
    • ATP-dependent chromatin remodeling complexes SW1/SNF (can distrupt histon-DNA –complexes)
  • 14.  
  • 15.
    • TdT gene is initially down-regulated when ikaros dislocates the transcriptional activator Ets-1 from the TdT promotor.
    • Down-regulation enhanced by an ikaros –dependent hetrochromatin formation at the TdT locus.
    • Ikaros can keep the gene in an inactive state through recruitment of NURD .