Li et al, found that platelets express mRNA for death ligand TRAIL , death receptors TNFR1, DR3 , DR4 and DR5 , and adapter proteins TRADD and RIP . In contrast , Fas receptor and Fas ligand were not detected in platelets as determined by mRNA and immunoblot and anti-Fas antibodies had no eﬀect on platelets.
ᴪ m In normal undamaged nucleate cells, mitochondria have a high ᴪ m; breakdown of ᴪ m is characteristic of early apoptosis . ᴪ m in platelets can be measured by the cell-permeable lipophilic cationic dyes JC-1 and DiOC6 . Using JC-1 , we have demonstrated depolarization of ᴪ m in PCs starting from days 13_14 of storage.
Cytochrome c, Diablo/Smac and Apaf-1. Cytochrome C and Apaf-1 have been found by immunoblot in whole lysates of fresh nonactivated platelets.